Relationship between radiation dose and chromosome aberration frequency – Calibration curves

Piotr KnappPiotr Wróblewski

Laboratory of Radiation Biology

Supervisors: Ekaterina Zaytseva, Polina Kutsalo, Marta Deperas-Kamińska

The fate of irradiated cell

Necroticdeath

After high dose

Somaticeffect

Healthy cell

Noeffect

Micronuclei

Mitotic death

No effect

Cancer

Somaticeffect

Geneticeffect

Carcinogeniceffect

Somaticeffect

Cell with damaged DNA

Correct repair

Incorrect repair

Unstable chromosomalaberrations

Apoptotic death

Stable chromosomalAberrations - Mutations

In the germ cells

Mutation path

Creation of dose dependency calibration curve of chromosomal aberrations in human peripherial blood lymphocytes exposed to protons beam (LET≤100keV/µm) performed at Phasotrone (LNP, JINR)

Biological dosimetry - Allow to assess an accidental radiation dose on base of the level of changes in chromosome structure.

Culture of irradiated

Colcemid

Fixation

48-50h

Watching

Staining with Fluorescence Plus

Giemsa

An example of cell stained with Giemsa dye with dicentric

chromosome.

DOSE [Gy]

Aberration per cell

Marta Deperas-Kamińska

Polina Kutsalo

Ekaterina Zaytseva

Relationship between radiation dose and chromosome aberration frequency – Calibration curves

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Transcript of Relationship between radiation dose and chromosome aberration frequency – Calibration curves

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