Radiation-resistance? There's a Gene for That.

Radiation-resistance?

There's a Gene for That.

New York City Wetware iGEM Team

Extraordinary Biology NumbersExtremophile Numbers

Extraordinary Synthetic Biology Numbers

130 Celsius 130 MPa

1.08-mega-base pair 4 Days

Deinococcus radiodurans (D. rad)

One to two hours of acute six to ten Gray radiation exposure.

12,000 Gray!!!!!

Life of the Cell

A Bad Day

Base Damage Single Strand Break Double Strand Break

Active Site Damage

The Extraordinary Weapon (Kryptonite)

1. H2O H· + OH·

2. OH· + OH· H2O2

3. Fe2+ + H2O2 → Fe3+ + OH· + OH−

Generation of Hydroxyl Radical via the Fenton Reaction

What Makes D. rad So Special?

1. Cellular Manganese-Iron Ratios

2. Neutralization of Superoxide Radicals

3. Superior DNA Repair

O-Mn Fe

Three Basic Approaches

1. Cellular Manganese-Iron Ratios Mn Fe

2. Neutralization of Superoxide Radicals

O-Three Basic Approaches

3. Superior DNA Repair

recA Repair Protein

Making E. coli Radioresistant

Mn/Fe O-

DNA Repair

Our MegaBrick

Our Biobricks•ruvA

•ruvB

•radA

•tpx

•ruvA

Future Applications

Waste Hungry Bacteria MegaBrick Clean Earth

Bioremediation

Future Applications

MegaBrickStem Cells Sick Person Healthy Person

Medical Applications

in vitro meat and moral traditions

Kosher Vegetarian Vegan

Legal Precedent: Gelatin

Legal Precedent: Rennet

Moral Considerations

• Ought a vegan or vegetarian to eat meat?• Ought a kosher-observer to eat pork?• Does cultivating meat without encountering

an animal desensitize us as eaters? Does it undermine the relationship between ourselves and animals?

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Radiation-resistance? There's a Gene for That.

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