Post on 08-Aug-2020
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PREPARATION OF MANGO LEAF EXTRACT NIOSOMES
MISS JUTAMAS JIARANAIKULWANITCH
MISS NUTTA TEERANITI
A SPECIAL PROJECT SUBMITTED IN PARTIAL FULFILENT
OF THE REQUIRREMENT FOR
THE BACHELOR DEGREE OF SCIENCE IN PHARMACY
FACULTY OF PHARMACY
MAHIDOL UNIVERSITY
2005
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Abstract
Preparation of Mango Leaf Extract Niosomes
Jutamas Jiaranaikulwanitch, Nutta Teeraniti
Project advisor : Varaporn Junyaprasert*, Nuntavan Bunyapraphatsara**, Uracha Rungsardthong***
*Department of Pharmacy, Faculty of Pharmacy, Mahidol University
** Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University
***National Nanotechnology Center, National Science and Technology Development Agency
Keyword : Niosome, Mangiferin, Reverse phase evaporation, Transdermal delivery
Mangiferin is an active substance in mango leaf (Mangifera indica Linn.) extract having
anti-oxidant, anti-inflammatory and anti-viral activities. The purpose of this study was to develop
niosomes of mango leaf extract to enhance transdermal delivery. Various factors affecting the
drug entrapment of niosomes were investigated. These factors included the use of Solulan C24
as a stabilizer, size reduction by high pressure homogenizer, concentration of extract in lipid and
total concentration of extract and lipid in formulation. Mango leaf extract niosomes were
prepared by a reverse phase evaporation method (45 ºC, 1 h), using 1:1 Span 60 and
cholesterol as wall forming agents and diethyl ether as a solvent. The results indicate that adding
Solulan C24 at 5 mole% of total lipid resulted in the smaller size niosomes with better stability.
Using high pressure homogenizer (200 bars, 3 cycle), the size of the obtained niosomes was
reduced to 270-500 nm with narrow size distribution. When analyzing the entrapped mangiferin
in nisomes by high performance liquid chromatography (HPLC), it was found that niosomes of
Solulan C24 had lower drug lose during size reduction. When the concentration of extract in lipid
increased, the drug entrapped increased. The entrapping efficiency was 39.45-73.17 % by
weight and % drug loading was 0.32-6.63 % weight by weight of total lipid in formulation. In
formulation of 36.67 % weight by weight of lipid of crude extract, the highest drug entrapped,
entrapping efficiency and % drug loading were obtained when using the total concentration of
extract and lipids at 5.03 % weight by volume.