Practical Review Practice( SemI)

Advanced Placement BiologyLaboratory Review Sheet

Dissolved oxygen simulation

http://www.phschool.com/science/biology_place/labbench/lab12/varlight.htmlPlay the simulation.Relate to an aquatic ecosystemHow does dissolved oxygen relate to primary productivityHow do the following variables affect dissolved oxygen

TemperatureSalt water and fresh water?Running and still

Question and answers

If the water temperature increases, the dissolved oxygen________

DecreasesIn general running water has ________

( more or less) oxygen than still water More

Nomogram

Read the % saturation

For a temperature of 25oC, what is the % saturation ?For a temperature of 25oC, what is the dissolved oxygen?

Class Microscope. http://www.microscope-microscope.org/basic/microscope-parts.htm

Identify the followingObjective lensesCondenserMechanical stage contraolFine AdjustmentCoarse AdjustmentRotating noewpieceBe able to identify:the following

OilHighLowScanning

How to prepare a wet mount. What are these steps?

What is this cell?

Starfish egg cell

Is this cell a eukaryote or a prokaryote?

What s the difference between these two pictures of Spirogyra? How would Spirogyra be classified?

What is Volvox?

Why does this show evolution?

Spectrophotometer

http://www.umd.umich.edu/casl/natsci/slc/spec21.swf - simulation

Spectrophotometer

What is absorbance?What is percent transmittance?

What colors are associated with the wavelengths of visible light

Chromatography with a Flair

Chromatography

What is the matrix?What is the solute?What is the solvent?What is the basis of separation?What is the Rf value?

Gel electropohoresis

What is an electrophoresis chamber ?

Describe how to put the sample in the wells?

The sample is loaded at this end of the gel box

This shows the molecules moving toward the

DNA gel

What is the stain in the picture?

What is the marker used with this experiment?

DNA Electrophoresis

Which is the marker?

Micromeasurement

What is the reading on this micropipet?

Micropipet Use( review)

http://www.fhcrc.org/education/hutchlab/lessons/use.html

Review how to use the micropipet

What is an isolation streak?

What is flaming?

What is the shape of these bacteria? Which one is gram positive?

What type of bacteria are these?

What type of bacteria are these?

What type of bacteria are these?

Kirby Bauer

What is resistance , what is sensitivity?