1

PIF Supplement Workflow Benefits

and Performance Data

Bio-Rad Laboratories

Food for Thought Newsletter, June 2020

2

Workflow Benefits

3

A Fully Harmonized Solution

Matrices

Targets

Screening Confirm.

Harmonized Infant formula without probiotics Infant formula with probiotics Ingredients

Harmonized Salmonella Cronobacter

Harmonized iQ-Check Kits RAPID’media

4

Benefits of 1:4 Dilution

3.75 kg / bag 2.25 kg / bag 1.5 kg / bag

375 g

1:10

375 g

1:4

375 g

1:6

Make your technicians happier with 1:4 dilution

5

Workflow Benefits

Save Time

• Technician handling

Save Space

• Incubators

Save Money

• Less media

• Less energy

• Less waste treatment

Save Energy

• For heating media

Save Waste

• Less media

A small 1:4 dilution factor for big benefits

6

Summary

PERFORMANCE

EASE OF USE

REASONABLE COST

Very low level of detection, even in

challenging conditions:

Large sample size

Stressed bacteria

Large sample size suitable method

No additional materials / technicians

Co-enrichment Salmonella & Cronobacter

Reagent cost

Consumable & material cost

Labor cost

7

Performance Data

AFNOR Validation Data

RAPID’Sakazakii

8

RAPID’Sakazakii AFNOR Performance Data

9

RAPID’Sakazakii AFNOR Performance Data

Sensitivity Study

RLOD Study Better sensitivity

than the reference

method

No false

positive

results

10

RAPID’Sakazakii AFNOR Performance Data

AFNOR certificate (avalaible) Validation Summary Report

(soon available)

11

Performance Data

AFNOR Validation Data

iQ-Check Cronobacter

12

iQ-Check Cronobacter AFNOR Performance Data

• AFNOR validation extension:

– 375 g sample size

– 1:4 dilution in BPW pre-warmed + PIF

Supplement

– 18–26 hr single enrichment @ 37 ± 1°C

– Free DNA Removal Solution (FDRS)

– APF Fast: “Crono Fast”

– 72 hr storage

• Matrices

– Infant formula and cereals w/wo

probiotics and ingredients

– Environmental samples

13

Sensitivity Study

Category SE Alt % SE Ref % RT % FP %

1 – 30 g regrowing 94.6 100.0 96.7 8.7

2 – 375 g + PIF 100 73.5 86.4 0.0

3 – Environmental 100.0 100.0 100.0 0.0

iQ-Check Cronobacter AFNOR Performance Data

14

RLOD

1 – 30 g regrowing

2 – 375 g + PIF

3 – Environmental

iQ-Check Cronobacter AFNOR Performance Data

15

AFNOR certificate (avalaible) Validation Summary Report

(soon available)

iQ-Check Cronobacter AFNOR Performance Data

16

Thank You