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Patronin Regulates thePatronin Regulates theMicrotubule Network byMicrotubule Network by

Protecting Microtubule Minus Protecting Microtubule Minus EndsEnds

Sarah S. Goodwin and Ronald D. ValeCell 143, 263–274, October 15, 2010

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Tubulin assembles into polar filaments with dynamic plus and minus ends.

However, in living cells, most microtubule minus ends are static.

Goodwin and Vale identify Patronin as a protein that stabilizes minus ends and protects them from depolymerization in vivo.

They further show that purified Patronin binds selectively to minus ends and shields them from a microtubule depolymerase, Kinesin-13.

Patronin contributes to • proper organization of the microtubule cytoskeleton and • formation of the mitotic spindle,

indicating that these structures are regulated by competing actions of destabilizing and stabilizing proteins acting on minus ends

• Patronin protects microtubule minus ends from depolymerases in vivo

• Without Patronin, microtubule organization is defective in interphase and mitosis

• In vitro Patronin can protect minus ends from Kinesin-13-catalyzed depolymerization

• Similar to plus ends, stabilizing and destabilizing proteins regulate minus ends

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Brian P. O'Rourke David J. Sharp Microtubule Dynamics: Patronin, Protector of the Minus EndCurrent Biology, Volume 21, Issue 2, R77-R79, 25 January 2011

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