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Pathway analysis with Metaboanalyst

Stephen Barnes, PhDProfessor of Pharmacology & Toxicology

sbarnes@uab.edu

Find the data in the mummichog file

Mac users:  mummichog‐1.0.7 is in the Applications folderOpen it to locate 1467593683.63.Grubbs_diet_neg_2000

Open the tsv folder

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Files in mummichog results folder

Identification of each observed ion

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Select and copy the KEGG IDs

Go to Metaboanalyst

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Transfer the KEGG IDs into the box, select the input type

Listed metabolites 

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Choose a species

Statistical analysis selection

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Significant pathways 

Steroid biosynthesis

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One carbon metabolismhttp://www.genome.jp/kegg/compound/

5,10‐Methenyl‐THF

10‐Formyl‐THF 5‐Methyl‐THF

DHF

Folate

THF

5‐Formimino‐THF

5,10‐Methylene‐THF

5‐Formyl‐THF

Krebs cycle

Pyruvate

Malate

oxaloacetate

succinate

fumarate

citrate

cis‐aconitate

isocitrate

2‐oxoglutarate

thiamine diphosphate

2‐Hydroxyethyl‐ThPP

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Mummichog "view" of metabolismGrubbs_diet_neg

Mummichog pathwaysGrubbs_diet_neg

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Questions?