Packing and Shipping

Prevent Disease, Disability and Premature Death

Objectiveهدف

Given specimens collected from the field, demonstrate proper sorting, counting, and preparation for shipment.

Prevent Disease, Disability and Premature Death

Overviewمرور

Processing CollectionsPacking and Shipping

Prevent Disease, Disability and Premature Death

Why Does it Matter

Proper packing and shipping of arthropod specimens ensures fast accurate identifications.

Prevent Disease, Disability and Premature Death

Why does it matter

Improper packing and shipping leads to problemsWhat can go wrong??

Damaged specimens due to overcrowdingStale specimens -- older ones become brittleSending male mosquitoes or “trash” bugsIncorrect labels

Bad packing and shipping produces bad results and wasted time

Prevent Disease, Disability and Premature Death

How to process collections

Collect specimens from traps as early as possibleKill specimens by placing in the freezer for several hours

Prevent Disease, Disability and Premature Death

Processing collection

Sort the specimens:1. Separate the mosquitoes from the “trash

bugs”-mosquitoes have one pair of wings-mosquitoes have a proboscis-the proboscis has scales-mosquito wing veins have scales

Prevent Disease, Disability and Premature Death

Processing collection

Some common insects mistaken for mosquitoes:

MidgesCrane fliesParasitic wasps

Prevent Disease, Disability and Premature Death

Packing and ShippingSort the specimens:1. Separate the mosquitoes from the “trash bugs”2. Separate the male mosquitoes from the female mosquitoes

Males have bushy antennae or “mustaches”Males have long palpsCulicine females have short palpsWatch out for female Anophelines

They have long palps

Prevent Disease, Disability and Premature Death

Male or Female?

Prevent Disease, Disability and Premature Death

Male or Female?

Prevent Disease, Disability and Premature Death

Mosquito Surveillance Program

1. Separate the mosquitoes from the “trash bugs” 2. Separate the male mosquitoes from the female mosquitoes

3. Pack the specimens:Pack the female mosquitoes into petri dish containersOnly place 20-40 female mosquitoes in each containerDo not ship males, wet specimens, other species

Prevent Disease, Disability and Premature Death

Packing and ShippingItems needed to properly pack adult female mosquito specimens

TissuesPetri dishesTapeLabels

Packing and Shipping

Place tissue on bottom half of petri dish

Packing and Shipping

Put female mosquitoes on tissue paper without allowing them to touch

Packing and Shipping

Place another tissue on the mosquitoes

Packing and Shipping

Squeeze petri dish lid on the bottomCut excess tissue paperTape petri dish closed and add label

Packing and Shipping

Items needed to properly pack larval mosquito specimens

AlcoholVialsTapeLabels

Packing and ShippingHeat water to kill mosquito larva in

Hot but not boilingPlace larvae in hot waterRemove larvae after a few secondsPlace larvae in 70-80% alcoholTape lid securely and add label

Packing and ShippingLabeling

Name of location/installationCollection siteMethod of collectionCollection dateCollector’s name

Prevent Disease, Disability and Premature Death

Summary

Processing CollectionsPacking and Shipping

Prevent Disease, Disability and Premature Death

Questions?

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