Bay scallop genetic diversity and population structure in

Massachusetts

Steven RobertsSchool of Aquatic and Fishery Sciences

University of Washington

Outline

•Beginning

•Marker Development

•Populations / Enhancement

•Conclusions

• ESTs = 7057

• CoreNucleotides = 85

• Contigs 845

• Singletons 2746

• Unique Seqs 3591

Individual clones sequencedD-hinge 671Met 61Set 932Adductor 367Gonad 58

8% sequences <200 bpAverage length - 719 bp970 singletons217 contigs

NCBI Present StatusLibrary Characteristics

Enhancement

Population Differences

Self-assignment

Only Adults Random = 11%Chatham = 60%

Assessing Enhancement

private alleles showing up post enhancement

Summary

• Identify markers that effectively identify all potential parent populations

• functional markers (SSR-ESTs, SNPs)

• Isolation coupled with life history can dramatically affect genetic diversity

• Temporal (yearly) variation is interesting and critical for understanding diversity