Northwest Microbiology Conference 2018 University of Salford - Salford Quays Campus 16th March 2018

WELCOME!

Welcome to Northwest Microbiology 2018 Northwest Microbiology returns! Welcome back to the University of Salford’s Salford Quays campus for the first time since 2014. For those new to this conference series, Northwest Micro was setup as a collaboration between Northern Universities with the intention of giving Early Career Researchers - our PhD students and Postdocs - an opportunity to showcase their amazing microbiology research.

This year, we have 120 attendees from across 7 institutions. We hope you’ll use this opportunity to interact with each other - share your research, your ideas, develop new collaborations.

Future Conferences We are keen to make this a regular (annual?) event. Please do give us your feedback, through the online survey that will be circulated by email after the conference. Your suggestions and opinions will serve to improve future conferences.

Sponsors This event is free-of-charge to make it accessible to all students. This would not be possible without the kind support from the Society for Applied Microbiology and the Microbiology Society, and from our industrial sponsors, all of whom have stands in the foyer. Please visit them - you may find they have products that are useful for your projects, and you may even win some prizes!

Social Media We encourage you to share through the usual social media platforms (we suggest the twitter hashtag #NWMicro18. Please be aware that some speakers / poster presenters may not wish for their research to be posted online - these will be clearly marked if so.

NORTHWEST MICROBIOLOGY CONFERENCE 2018

PROGRAMME

08:30 - 09:30 REGISTRATION

09:30 Welcome & Housekeeping

09:45 - 10:30 Session 1: Poster Lightning Talks Chair: Christina Bronowski

1 minute talks from all Poster presenters

10:30 - 11:15 COFFEE BREAK & POSTER VIEWING

Offered Talks 1: Microbial Communities Chair: Chloe James

11:15 - 11:30 The Importance of Method Standardization for Human Microbiota Studies

Anna Pulawska-Czub

11:30 - 11:45 Development of the Chicken Intestinal Microbiome Peter Richards

11:45 - 12:00 Molecular Epidemiology of Tick-Borne Haemoparasites in Nigerian Sheep

Babagana Mohammed Adam

12:00 - 12:15 Impact of maternally derived antibodies and infant microbiota on the immunogenicity of rotavirus (RV) vaccines in African, Indian and European infants. Initial results from the RoVI study

Christina Bronowski

12:15 - 12:30 Low dose Trichuris muris infection impairs host immune control of Streptococcus pneumoniae nasopharyngeal carriage.

Alice Law

12:30 - 14:00 LUNCH

Offered Talks 2: Novel Antimicrobials and Antiparasitics

Chair: Prof. Jay Hinton

14:00 - 14:15 Heparins mimetics inhibits malaria parasite Plasmodium falciparum growth and merozoite invasion of the red blood cells in vitro

Muqdad Hmoud

14:15 - 14:30 Antimicrobial activities of novel Chrysin adducts Nicholas Omonga

14:30 - 14:45 Novel Roles for Skin lipids in Staphylococcal Survival Jo Moran

14:45 - 15:15 COFFEE BREAK & POSTER VIEWING

Offered Talks 3: Microbial Genomics and Transcriptomics

Chair: Ian Goodhead

15:15 - 15:30 Identification of a novel phage infection immunity protein by transcriptomic analysis of a Salmonella prophage

Sian Owen

15:30 - 15:45 Microbial mutation rates have evolved a plastic association with population density across domains of life

Huw Richards

15:45 - 16:00 The role of the RNA chaperone ProQ in the Gram negative bacterium Pasteurella multocida.

Emily Gulliver

16:00 - 16:45 KEYNOTE ADDRESS Prof. Miren Iturriza-Gomara

16:45 - 17:00 Closing Remarks & Prizes

17:00 - 18:30 EVENING RECEPTION

NORTHWEST MICROBIOLOGY CONFERENCE 2018

KEYNOTE ADDRESS Professor Miren Iturriza-GomaraProfessor of VirologyInstitute of Infection and Global Health, University of Liverpool

Enteric vaccines: virus diversity versus population diversity

Rotavirus is the commonest cause of severe diarrhoeal disease in children and a major contributor to child morbidity and mortality worldwide. The global introduction of rotavirus vaccines has had significant impact in reducing this burden. However, despite their impact, currently available rotavirus vaccines, like other enteric vaccines such as polio, are less effective in low-income, high-burden countries than in higher income settings. Limitation of vaccine effectiveness in the very countries which need them most could result in a significant continuing burden of potentially vaccine preventable deaths and disease. Multiple explanations for this disparity have been proposed, including virus diversity and host genetics, maternal antibody, age at vaccination, persistent exposure to enteric pathogens in the environment, altered gut microbiota and malnutrition and micronutrient deficiencies. This talk will summarise some of the data rotavirus disease burden and vaccine impact and current research aimed at understanding the factors that may be responsible for enteric vaccine underperformance in low income countries. Options for improving protection against diarrhoeal disease among children worst affected will also be discussed.

NORTHWEST MICROBIOLOGY CONFERENCE 2018

ABSTRACTS: ORAL PRESENTATIONS Offered Talks 1: Microbial Communities

The Importance of Method Standardization for Human Microbiota StudiesAnna Pulawska-Czub

NIHR Health Protection Research Unit in GI, University of Liverpool

Human intestine contains diverse microbiota with significant role in health and disease. Determining bacterial composition in faecal samples through DNA sequencing is a multistep process where choice of analytical procedures can influence and bias final observations.Systematic approach was taken to study the impact of sample storage conditions [neat or homogenized with stabilization buffer: DNAgard, PSP or EtOH in various temperatures for up to 5months]; choice of DNA extraction method [commercial kits: Stratec, ZYMO RESEARCH, QIAGEN, MP Biomedicals] and amplicon target for next-generation sequencing on MiSeq platform [V4 or V3V4].Results demonstrated that homogenization of faecal samples with selected stabilization buffers contributed to better bacterial community preservation in higher temperatures comparing to those left neat or submerged in EtOH. Bacterial phyla abundances varied across DNA extraction methods with highest proportions of Actinobacteria and Firmicutes recovered with ZYMO and Bacteroidetes, Cyanobacteria and Proteobacteria with QIAamp. Finally, significant differences were observed between abundances of over 20 families when alternative variable regions of 16S rRNA gene were sequenced.These data highlight the importance of consistency of protocols among microbiota studies in order to enable cross-comparability. It also provides useful information to help interpret differences observed between studies.

Development of the Chicken Intestinal Microbiome

Richards, P.; Wigley, P.; Fothergill, J; Bernardeau, MUniversity of Liverpool

The intestinal microbiome is a crucial factor in the development of the intestinal immune system and host metabolism. An understanding of how microbial communities develop under normal circumstances can provide a rational basis for probiotic interventions which bolster early immune maturation and metabolic capabilities. The development and succession of the ileal and caecal microbiome in three breeds of broiler chickens between 0 and 42 days post hatch (d.p.h) was analysed using an Illumina MiSeq run. DNA extracted from luminal samples at 0, 3, 7, 14, 21, 28 and 42 d.p.h and mucosal samples at 14, 21, 28 and 42 d.p.h was submitted for sequencing of the V4 region of the 16S rRNA gene. Our results show that the early intestinal microbiome has poor diversity with one or two taxa contributing the majority of sequences. From 3 d.p.h, microbiome diversity begins to increase with differences between the caecal and ileal microbiomes becoming apparent. Over time the caecal and ileal microbiomes diverge and stabilise by 21 d.p.h and 42 d.p.h respectively. Differences in taxa between the caecum and ileum reveal a large functional diversity between the two compartments defining different roles in host metabolism.Significant differences between the caecal mucus and lumen microbiome were identified. Temporal differences associated with the presence of epithelial associated segmented filamentous bacteria were identified between ileal mucus and luminal samples.

Molecular Epidemiology of Tick-Borne Haemoparasites in Nigerian Sheep

Babagana Mohammed Adam1, Vincenzo Lorusso1,2, , Kevin Bown1, Michiel Wijnveld3, Richard Birtles1

1. University of Salford Tick Infections (USALTI) Group, School of Environment and Life Sciences, University of Salford; 2. Global Research & Medical Division, Vetoquinol, Paris, France;3. Centre for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Austria

Tick-transmitted pathogens (TTPs) impose serious constraints to livestock health and productivity in sub-Saharan Africa, including Nigeria. The aim of this study was to assess the occurrence of TTPs in domestic ruminants from North-Western Nigeria, focusing on the so far more neglected species of sheep, using a sensitive molecular approach. 257 whole blood samples were collected from sheep in Kachia grazing reserve, Kaduna State. Detection of TTPs was conducted by means of PCR-based reverse line blotting, targeting six genera of microorganisms including bacteria and apicomplexan protozoa. 75.1  % of sampled animals were infected, with the great majority (78.2%) being affected by multiple infections. Theileria equi-like  was the most prevalent microorganism detected (66.3%), followed by  Rickettsia spp. (20.2%), Anaplasma centrale (17.5%), Theileria velifera (12.1%),  Theileria spp. (10.8%), Ehrlichia sp. Omatjenne (10.1%), Ehrlichia/Anaplasma spp.  (10.1%),  Theileria mutans (8.9%),  Theileria sp. MSD4 (5.8%), Bartonella spp. (3.1%), Babesia bovis (2.7%), Babesia spp. (1.25%), Babesia caballi (0.45%), Ehrlichia ruminantium (0.4%) and Rickettsia spp. of the “thypus group” (0.4%). Lambs were significantly less infected than juvenile and adult sheep. Numerous TTPs were identified in sheep from NW Nigeria that could cause disease and serious production losses. The detection of several zoonotic agents (i.e. Rickettsia spp. and Bartonella spp.) warrants further characterisation studies to better assess risk for human infections in the area.

Impact of maternally derived antibodies and infant microbiota on the immunogenicity of rotavirus (RV) vaccines in African, Indian and European infants. Initial results from the RoVI study

Christina Bronowski1, Edward Parker2, Ira Praharaj3, Alistair Darby4, Nigel Cunliffe1, Miren Iturriza-Gomara1 & the RoVI consortium.

1. Institute of Infection and Global Health, University of Liverpool, 2. Imperial College London, 3. Christian Medical College, Vellore, 4. Institute of Integrative Biology, University of Liverpool

Rotavirus is the commonest cause of severe gastroenteritis in infants under five years of age worldwide. RV vaccination has led to significant reductions in infant mortality. However, RV vaccine efficacy is significantly lower in low income countries, in the very populations that sustain the highest burden. The reasons for the reduced responses are poorly understood. Evidence suggests that alteration of the infant gut microbiota at the time of vaccination may be associated with poor live oral vaccine take and immunogenicity.The study enrolled birth cohorts in three countries: Malawi and India, infant populations in whom RV vaccine efficacy is poor, and the United Kingdom, where vaccine efficacy is high. Infants were followed from birth up to 16 weeks of age. Blood and breast milk samples are collected to investigate the impact of maternal antibody transfer on seroconversion. Serial stool samples are collected to examine gut microbiota development, early exposure to natural RV infection and RV vaccine virus shedding post immunisation. Gut and systemic inflammation at the time of vaccination are also being assessed. Here we present preliminary microbiota results from all three cohorts. This study will ultimately provide data on how maternally derived immunity and the intestinal microbiota, impact on RV vaccine take and immunogenicity in the infant, providing novel data on the maternal microbiota imprinting of the infant gut microbiota in different populations.

NORTHWEST MICROBIOLOGY CONFERENCE 2018

Low dose Trichuris muris infection impairs host immune control of Streptococcus pneumoniae nasopharyngeal carriage.

A. Law; R. J. Flynn; R. K. Grencis; A. Kadioglu; D. R. Neill

University of Liverpool

Nasopharyngeal colonization by Streptococcus pneumoniae and soil-transmitted helminth (STH) infection are common childhood events in many areas of the developing world. It is likely that a substantial proportion of children worldwide are coinfected with pneumococcus and one or more helminths. Helminths modulate systemic host immunity in such a way that ensures chronic infection, with polarization towards a Th1 or T regulatory response often playing a crucial role. Given the importance of balance between these pathways in maintaining asymptomatic pneumococcal carriage, STH-driven immune perturbation may have a significant impact on carriage and susceptibility to invasive pneumococcal disease (IPD). Using a murine pneumococcal-Trichuris muris coinfection model, we investigated the dynamics of pneumococcal carriage and assessed immune responses during simultaneous STH infection. T. muris infection led to increased nasopharyngeal bacterial loads and enhanced dissemination of pneumococci to the lungs and blood. Higher levels of IFNg and TNFa were observed in the respiratory tissues of co-infected mice in comparison to singly-infected mice, suggesting that T. muris-driven host immune modulation plays a role during co-infection. These data address a previously unrealised developing world health issue and data from this study could eventually inform treatment options to combat IPD as well as enhance our understanding of pneumococcal disease dynamics in the developing world.

NORTHWEST MICROBIOLOGY CONFERENCE 2018

Offered Talks 2: Novel Antimicrobials and Antiparasitics

Heparins mimetics inhibits malaria parasite Plasmodium falciparum growth and merozoite invasion of the red blood cells in vitro

Muqdad Hmoud1, Mark Skidmore2, Edwin Yates3 and Paul Horrocks1

1. Institute of Science and Technology in Medicine, Keele University, Staffordshire, ST5 5BG, UK. 2. School of Life Sciences, Keele University, Staffordshire, ST5 5BG, UK. 3. Institute of Integrative Biology, University of Liverpool, L69 72B

Despite the global effort to decrease mortality and morbidity, malaria still causes 430000 deaths and more than 200 million infection cases according to World Health Organisation report 2017. Plasmodium falciparum is the most lethal malaria parasite among other malaria spp. Antimalarial drug resistance has emerged in many malaria affected areas in the world.There is an urgent need to develop new antimalarial therapeutics to overcome antimalarial resistance and to support current advances in antimalarial drug development. Heparin and other glycosaminoglycans have shown antiplasmodial activity against P.falciparum.This activity seems to be mediated by interfering with merozoite surface proteins (MSPs) and erythrocyte membrane binding proteins (PfEMP) resulting in invasion blocking of the erythrocyte.The antiplasmodial activity of heparin has not been exploited therapeutically due to its high anti-coagulation property. Here we report a novel heparin mimetic Glycosaminoglycans that have in vitro antiplasmodial activity. This activity was assessed and compared with their anti-coagulation property offering a new approach to malaria treatment as an adjunct therapy.

Antimicrobial activities of novel Chrysin adductsNicholas OmongaUniversity of SalfordA series of new 7-O-Chrysin derivatives were synthesised. Seventeen compounds were screened against eight bacteria: Escherichia coli 2513, Pseudomonas aeruginosa 2513, Bacillus cereus 2513, Staphylococcus aureus 25923, MRSA 252, Enterococcus faecalis 2513, Pseudomonas fluorescens 2513 and Klebsiella pneumonia 2513 and one fungus - Candida albicans MTCC 227 using Gentamycin and Fluconazole as standard drugs for antibacterial and antifungal studies. Compounds containing ketone groups (C1), methyl groups (C2) and nitrate groups (C3) indicated bactericidal inhibition against a broad spectrum of bacteria tested. S. aureus, E. coli, B. cereus and E. faecalis being the most susceptible bacteria to inhibition by these compounds. MIC of C1 against these bacteria was 25, 50, 50 and 50 µg / Ml respectively. C2 showed bacterial inhibition to these bacteria at MIC values of 50, 100, 100 and 100 µg / mL respectively. C3 showed bacterial inhibition at MIC values of 12.5, 12.5, 25.0 and 50 µg / mL respectively. MIC values indicated by these compounds were better than those indicated by Chrysin and were comparable to those showed by the standard drug Gentamycin. C1 and C3 also inhibited the growth of the drug-resistant bacteria MRSA at doses of 65 and 100 µg / mL respectively. C1 and C3 also showed superior antifungal activities against the pathogenic fungus Candida albicans at MIC values of 50 µg / mL respectively compared to the standard antifungal drug Fluconazole (MIC 125 µg / mL). 7-O-Bromochrysin and 7-O-Alkylchrysin derivatives of Chrysin showed weak antimicrobial activities (MIC: 125 µg / mL) against all bacteria and fungus tested.

Novel Roles for Skin lipids in Staphylococcal SurvivalMoran J, Xizhang Z, Horsburgh M.University of LiverpoolStaphylococci are commensal colonisers of human skin, but several species are also notable opportunistic pathogens, causing diseases ranging from superficial skin abscesses to life-threatening sepsis and pneumonia. Improving our understanding of the factors that enable staphylococcal colonisation of skin may inform strategies to prevent transmission or identify pathways for the design of new antimicrobials to treat infections caused by multidrug resistant Staphylococcus aureus.Research into skin lipids has previously focused on the minor fraction that were long understood to be antimicrobial and influence survival of staphylococci by diminishing cell surface functions. We have examined several other topical lipids to determine their contribution to survival of staphylococci, including squalene and cholesterol. The abundant skin lipid squalene, present in sebum, was revealed to be antimicrobial to staphylococci under low iron conditions by causing iron starvation. Squalene also reduces expression of the pigment of S. aureus thereby decreasing survival from oxidative stress and antimicrobial peptides. Squalene is a precursor for cholesterol biosynthesis and the latter increases survival of staphylococci from antimicrobial skin lipids such as sapienic and linoleic acids. Transcriptomic analysis reveals that cholesterol alters the staphylococcal response to sapienic acid, changing described pathways in cell wall turnover that could act to maintain cell surface function and limit antimicrobial activity. Together, these new descriptions of skin lipid actions could inform key functions of the host barrier that determine the composition of the human skin microbiome.

NORTHWEST MICROBIOLOGY CONFERENCE 2018

Offered Talks 3: Microbial Genomics and Transcriptomics

Identification of a novel phage infection immunity protein by transcriptomic analysis of a Salmonella prophage

Owen, SV and Hinton JCDInstitute of Integrative Biology, University of Liverpool

Bacterial prophages have long been recognised to carry accessory genes that enhance the fitness of their hosts, known as lysogenic conversion. For example, many important pathogens rely on prophage-encoded virulence genes to cause disease. Genes which are expressed during lysogeny are frequently associated with lysogenic conversion phenotypes, so transcriptomic analysis of the prophage regions of bacterial genomes presents an opportunity to identify novel prophage accessory genes.The transcriptome of the BTP1 prophage of Salmonella Typhimurium revealed several genes of unknown function that were highly expressed in infection-relevant conditions, suggesting they may contribute to the biology of the bacterium. Genetic inactivation of one of the putative accessory genes made the S. Typhimurium susceptible to infection by phage P22. Heterologous expression of the accessory gene in a naive host conferred broad-spectrum phage immunity, making the lysogen resistant to diverse and unrelated phages. Homologs of the accessory gene were identified in the genomes of diverse enteric bacteria, suggesting the accessory gene represents a novel family of phage superinfection immunity proteins.This study shows that transcriptomic analysis of prophage regions can identify and assign function to novel phage accessory genes. More broadly, this study demonstrates the potential of next generation sequencing technology to inspire a renaissance in the study of bacteriophage molecular biology.

Microbial mutation rates have evolved a plastic association with population density across domains of life

Richards, H; Krašovec, R; Gifford, DR; McBain, AJ; and Knight, CG

University of Manchester

How and why the rate of spontaneous genetic mutation varies is a fundamental and enduring evolutionary and biological issue. Across organisms, mutation rates have evolved a negative association with effective population size. Yet, within any particular genotype, mutation rates can vary plastically with the environment. We find a strong negative association between mutation rate and population density in the last 75 years of published literature, comprising hundreds of mutation rates (estimated by phenotypic markers of mutation, fluctuation tests) from all domains of life and viruses. Our empirical tests of this association reveal density associated mutation-rate plasticity (DAMP) is present, but variable, in microbes from all domains of life. DAMP depends upon a protein scavenging the same mutagenic oxidised nucleotide (8-oxo-dGTP) in both domains tested. We find DAMP has evolved even at a fine evolutionary scale with significant variation in DAMP between 66 strains of Escherichia coli, where more closely related strains have greater similarity in their degree of DAMP. Such a widespread plastic association in such disparate organisms with such a highly conserved mechanism suggests that DAMP is pervasive across the tree of life. Since mutation rate, like numerous other critical microbial behaviours, responds to population density suggests that, for the de novo evolution of traits such as antibiotic resistance, social circumstances and evolutionary outcomes are tightly linked.

The role of the RNA chaperone ProQ in the Gram negative bacterium Pasteurella multocida.

Emily Gulliver1, Brandon Sy2, Julia Wong2, Deanna Deveson Lucas1, Ralf B. Schittenhelm3, David Powell4, Jai Tree2, Marina Harper1, John Boyce1

1. Infection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University, Victoria, 2. School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia, 3. Monash Biomedical Proteomics Facility, Monash Biomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Monash University, Victoria, 4. Monash Bioinformatics Platform, Monash University, Clayton, Victoria, Australia.

Hfq is a well-characterized RNA chaperone that is involved in riboregulation in bacteria, a process that involves sRNA/mRNA interactions that alter transcript stability and/or translational efficiency, thereby controlling transcript abundance and protein production. Recently, a second RNA chaperone called ProQ has been shown to play a critical role in stabilizing some sRNA/mRNA interactions. To assess the role of ProQ in the Gram negative bacterium P. multocida, we analyzed the transcriptome of a proQ TargeTron® mutant using RNA-seq. In total, 35 transcripts showed increased expression and 96 showed decreased expression. Predicted sRNAs and tRNAs were highly overrepresented in the differentially regulated genes, with 18 predicted sRNAs (p = 3.76x10-9), four with increased expression and 14 with decreased expression, and 17 tRNAs (p =3.78x10-11), all with increased expression, identified. Direct interactions between ProQ and one sRNA, five tRNAs and, 28 mRNAs involved in the production of ribosomal proteins, was confirmed using UV-crosslinking. The P. multocida ompA mRNA showed decreased production at the 3’ end; UV-crosslinking identified that this region also binds to ProQ. Interestingly, P. multocida ompA mRNA was found in hybrids together with other mRNA species, including hupA, indicating that binding between these RNA species is occurring.

NORTHWEST MICROBIOLOGY CONFERENCE 2018

POSTER PRESENTATIONS Poster FullName Organiza3on Title

1 YasminHilliam UniversityofLiverpool

Increasedtolerancetocontactlensdisinfec7ng

solu7oninclinicalisolatesofPseudomonasaeruginosafromkera77spa7ents

2 LauraWright UniversityofLiverpoolInterac7onsofmicrobeswithP.aeruginosainanar7ficialsputummodel

3 EsraaAldawood UniversityofManchester

Dissec7ngtheregula7onofthethreetandempromotersoftheEscherichiacolikps

geneclusteratchromosomallevel.

4 PoppyStevens UniversityofSalford

Characterisa7onofsandfly(Phlebotomusargen7pes)

microbiotalinkedtoLeishmaniainfec7onstatusacrossBihardistrict,India

5 SophieGlossop UniversityofHuddersfield

Bacterialgrowthcondi7onsaffecttheresponseof

culturedkera7nocytestoPseudomonasaeruginosa

extracellularmedia

6 RocioCanals UniversityofLiverpool

Iden7fica7onofkeytranscriptomicdifferencesbetweenglobalandAfrican

sequencetypesofSalmonellaTyphimurium

7 MohamedEshlak UniversityofSalford

An7microbialac7vityofPolyunsaturatedFaTyAcidsrelatedtoChronicWound

Infec7ons

NORTHWEST MICROBIOLOGY CONFERENCE 2018

8 FlavianeSantosdeSouza TheUniversityofSalford

OccurrenceofDWVvariantsinthes7nglessbeeMeliponasubni7da

popula7onthroughoutNEBrazil

9 CharloTeChong UniversityofLiverpool

Pep7doglycanModifica7onModula7ngResistance,

SurvivalandMetabolismofStaphylococcusaureus.

10 HamzaNHameed KeeleUniversityAn7leishmanialac7vityoftemperateplantsecondary

metabolites

11 ChowdhuryMehediHasan UniversityofLiverpool

Compensatoryevolu7onofthecostsofsingleversusmul7drugresistancein

experimentalAcinetobacterbaylyi

12 HebaBarnawi UniversityofManchesterRegula7onofMetalResistanceOperoninCampylobacterjejuni

13 LizethLacharme UniversityofLiverpool

Theembryonatedchickeneggasanalterna7veinfec7onmodelfor

Salmonella

14 HussainEbrahim UniversityofLiverpoolHormoneinterac7onswithPseudomonasaeruginosaurinarytractinfec7ons

15 OluwapamilerinYangomodou UniversityofSalford

GeneExpressionAnalysisandAn7microbialPoten7alofPlantSeedandNutOils

againstS.aureus

16 TerenceMarkham UniversityofSalfordTheexperimentalevolu7onofS.glossinidiusviaoxida7ve

stress

17 BlancaPerezSepulveda UniversityofLiverpool Sequencingof10,000

Salmonellagenomes

18 EljelaniSalim UniversityofSalford OccurrenceofCryptosporidiuminUKsheep

19 WaiYeeFong UniversityofLiverpoolOp7misingextrac7onofRNA

fromintra-macrophageSalmonella

Poster FullName Organiza3on Title

20 PolNadalJimenez UniversityofLiverpool Genomicanalysisofaninsectendosymbiont

21 JohnNewman UniversityofLiverpool

PhenotypicdiversitywithinPseudomonasaeruginosapopula7onscausingurinary

tractinfec7ons

22 AngharadGreen UniversityofLiverpool

Iden7fica7onofniche-specificvirulencefactorsviaexperimentalevolu7onofStreptococcuspneumoniae

23 SamClark UniversityofManchesterExploringtheac7vity

landscapeofacompletesetofan7microbialpep7des

24 VictoriaRimmer UniversityofManchester

Ar7ficialtearsolu7onincreasesvirulencedeterminantsof

Pseudomonasaeruginosabutatafitnesscost

Poster FullName Organiza3on Title

DELEGATE LIST FirstName Surname Affilia3on Email

Chloe James UniversityofSalford c.james@salford.ac.uk

Ian Goodhead UniversityofSalford i.b.goodhead@salford.ac.uk

Poppy Stevens UniversityofSalford

Elli Wright LiverpoolSchoolofTropicalMedicine Elli.Wright@lstmed.ac.uk

Heather Allison UniversityofLiverpool hallison@liverpool.ac.uk

Jo Fothergill UniversityofLiverpool j.fothergill@liv.ac.uk

Chris7na Bronowski UniversityofLiverpool 7nab@liverpool.ac.uk

Daniel Neill UniversityofLiverpool d.neill@liverpool.ac.uk

Yasmin Hilliam UniversityofLiverpool hlyhilli@liverpool.ac.uk

Miren Iturriza-Gomara UniversityofLiverpool miren@liverpool.ac.uk

Jay Hinton UniversityofLiverpool jay.hinton@liv.ac.uk

Angharad Green UniversityofLiverpool Angharad.Green@liverpool.ac.uk

Caisey Pulford UniversityofLiverpool hlcpulfo@student.liverpool.ac.uk

Laura Wright UniversityofLiverpool llwright@liverpool.ac.uk

Steven Kemp UniversityofLiverpool

Eman Alshehri UniversityofLiverpool e.a.alshehri@liv.ac.uk

John Newman UniversityofLiverpool

Rebecca Shears UniversityofLiverpool

Lauren Gordon UniversityofLiverpool hllgordo@student.liv.ac.uk

Alexander Predeus UniversityofLiverpool

Hussain Ebrahim UniversityofLiverpool hebrahim@liverpool.ac.uk

Sarah Withers UniversityofSalford s.b.withers@salford.ac.uk

Ian Roberts UniversityofManchester i.s.roberts@manchester.ac.uk

Bushra AlLawa7 UniversityofManchester bushra.allawa7@postgrad.manchester.ac.uk

Joe La7mer UniversityofSalford j.la7mer2@salford.ac.uk

Alice Law UniversityofLiverpool aelaw@liverpool.ac.uk

Rachael Antwis UniversityofSalford r.e.antwis@salford.ac.uk

stephen mar7n UniversityofSalford s.j.mar7n@salford.ac.uk

James Redfern MMU J.redfern@mmu.ac.uk

FirstName

Emma Dearing UniversityofLiverpool E.Dearing@liverpool.ac.uk

ELJELANI SALIM UniversityofSalford e.s.salim@edu.salford.ac.uk

Alex TompseT UniversityofSalford a.h.tompseT@edu.salford.ac.uk

Craig Winstanley UniversityofLiverpool C.Winstanley@liv.ac.uk

monica staniek LancasterUniversity m.staniek1@lancaster.ac.uk

Helen Gavillet MMU helen.gavillet@stu.mmu.ac.uk

Nadin Fathallah LancasterUniversity

Chris vanderGast MMU C.vanderGast@mmu.ac.uk

SAEED ALDOSSARI UniversityofLiverpool pssaldos@liverpool.ac.uk

Yasmine Kumordzi LancasterUniversity y.kumordzi@lancaster.ac.uk

SHAMSUL GULAMALI UniversityofLiverpool shamgali@liverpool.ac.uk

Hind Alrajeh UniversityofManchester

Rachel Forsyth UniversityofLiverpool floyd78@liv.ac.uk

Heba Barnawi UniversityofManchester heba.barnawi@postgrad.manchester.ac.uk

Reem Barnawi UniversityofManchester

Ayed Alshamamri UniversityofSalford A.E.M.Alshammari@edu.salford.ac.uk

Dennis Linton UniversityofManchester james.d.linton@manchester.ac.uk

Sian Owen UniversityofLiverpool sian.owen@liverpool.ac.uk

Blanca PerezSepulveda UniversityofLiverpool blanca.perez@liverpool.ac.uk

WaiYee Fong UniversityofLiverpool wyfong@liverpool.ac.uk

MohdZulkifli Salleh UniversityofManchester m.z.salleh@manchester.ac.uk

Xiaojun ZhuNatashaHussain Jafri UniversityofManchester

natasha.hussain-2@postgrad.manchester.ac.uk

Evita Mayasari UniversityofManchester evita.mayasari@postgrad.manchester.ac.uk

Gavin AckersJohnson UniversityofSalford g.ackersjohnson@edu.salford.ac.uk

Olayode Olatunji UniversityofManchester olayode.olatunji@postgrad.manchester.ac.uk

Julie Truman Qiagen julie.truman@qiagen.com

Terence Markham UniversityofSalford

Sean Goodman UniversityofLiverpool s.goodman@liverpool.ac.uk

AhmadReza Khan

Surname Affilia3on EmailFirstName

Claire Scantlebury UniversityofLiverpool claire.scantlebury@liverpool.ac.uk

Victoria Rimmer UniversityofManchester victoria.rimmer@postgrad.manchester.ac.uk

Esraa Aldawood UniversityofManchester esraa.aldawood@postgrad.manchester.ac.uk

Adrian Cazares UniversityofLiverpool

Wendy Figueroa

jessica kevill UniversityofSalford j.kevill@edu.salford.ac.uk

Rocio Canals UniversityofLiverpool rcanals@liv.ac.uk

Emily Gulliver MonashUniversity emily.gulliver@monash.edu

Lewis Fisher UniversityofLiverpool lewis.fisher2@liverpool.ac.uk

Adrian Jervis UniversityofManchester adrian.jervis@manchester.ac.uk

Gregory Bulmer UniversityofManchester gregory.bulmer@postgrad.manchester.ac.uk

Hao Yu

Jiayi Tang

Farah Mughal UniversityofManchester farah.mughal@manchester.ac.uk

Hasan Chowdhury UniversityofLiverpool c.hasan@liverpool.ac.uk

Srijan Jindal UniversityofManchester srijan.jindal@postgrad.manchester.ac.uk

AngeziwaChunga Chirambo UniversityofLiverpool chirambo@liverpool.ac.uk

Oliver Standing

Sophie Glossop UniversityofHuddersfield

OluwapamilerinDamola Yangomodou UniversityofSalford o.d.yangomodou@edu.salford.ac.uk

Arthur Barnard

Josephine Moran UniversityofLiverpool moranj@liverpool.ac.uk

CharloTe Chong UniversityofLiverpool c.e.chong@student.liverpool.ac.uk

Mohamed Eshlak UniversityofSalford m.s.eshlak@edu.salford.ac.uk

Chris7an Harrison

Muqdad Hmoud KeeleUniversity m.k.hmoud@keele.ac.uk

Flaviane SantosdeSouza UniversityofSalford

Pol NadalJimenez UniversityofLiverpool

hanshuo lu UniversityofLiverpool hanshuo.lu@liverpool.ac.uk

Hamza Hameed KeeleUniversity h.n.hameed@keele.ac.uk

Surname Affilia3on EmailFirstName

Lizeth Lacharme UniversityofLiverpool lacharme@liverpool.ac.uk

BabaganaMohammed Adam UniversityofSalford B.M.Adam@edu.salford.ac.uk

Nicky Morgan UniversityofSalford N.L.morgan@salford.ac.uk

Sam Clark UniversityofBristol sc0271@my.bristol.ac.uk

Richard Birtles UniversityofSalford r.j.birtles@salford.ac.uk

Lauren Hafield MMU lauren.hafield@stu.mmu.ac.uk

Anna Pulawska-Czub UniversityofLiverpool annmaria@liverpool.ac.uk

Damian RiveT MMU d.riveT@mmu.ac.uk

Howard Foster UniversityofSalford h.a.foster@salford.ac.uk

Peter Richards UniversityofSalford pr8448@liverpool.ac.uk

Nicholas Omonga UniversityofSalford N.Omonga@edu.salford.ac.uk

Andrea Gbobaniyi UniversityofSalford

Mohammed Nagshabandi UniversityofManchestermohammed.nagshabandi@postgrad.manchester.ac.uk

Jodie Barber MMU jodie.barber2@stu.mmu.ac.uk

Geraldine Foster LiverpoolSchoolofTropicalMedicine geraldine.foster@lstmed.ac.uk

Jessica Balde

Huw Richards UniversityofManchester huw.richards@postgrad.manchester.ac.uk

sladjana malic MMU s.malic@mmu.ac.uk

Jonathan Butler MMU Jonathan.Butler@mmu.ac.uk

Elliot WhiTard MMU E.WhiTard@mmu.ac.uk

Natalie Callaghan MMU natalie.callaghan@mmu.ac.uk

Grace Crowther MMU G.Crowther@mmu.ac.uk

Niall Hickey MMU

Steven Parker UniversityofManchester steven.parker@manchester.ac.uk

Zachary Gander

Anthony Slate MMU anthony.slate@stu.mmu.ac.uk

Samina Naseeb UniversityofManchester samina.naseeb@manchester.ac.uk

Joels Wilson-Nieuwenhuis MMU j.wilson-nieuwenhuis@mmu.ac.uk

Patrick Dina

Surname Affilia3on EmailFirstName

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