Microscope Control with LabVIEW and IMAQ Vision

National Instruments Scientific Imaging Symposium

Morten Jensen NI Consulting Services

Agenda

• National Instruments Consulting Services• Microscope Control – First Steps• Microscope Start-up Kit• Future Microscope Kits (Compact Vision System)• Vision Builder for Automated Inspection• Q&A

What is National InstrumentsConsulting Services?

• Design consulting & reviews • Proof of concepts• Benchmarking • Feasibility studies • Startup assistance

• “Meet the Experts”• Cooperative risk management • Close support during development • Specialized training

Microscope Control – First Steps: Cell CountingFeatures: • IMAQ/Motion/DAQ Integration

• XY Axis Control (Manual & Auto)• Display Results and Center FOV• Automatic FOV Image Stitching

Cell Counting / Analysis• Threshold image• Over 50 parameters available for analysis

QuantitySizeLocationArea

Microscope Control – First Steps

Displayed:• Medical Design and

Manufacturing (’01, ’02, ‘03)• PittCon (’02, ‘03)• NI Week• 3 Shows in Europe

Next Step: Microscope Start-up Kit

Camera

ControllerUnit

PXI and LabVIEW Vision

Microscope

RS-232

C-Mount

NI-IMAQ

Features• Low-cost

Microscope Monitoring and Control Tool

• Accurate Sample Analysis Capability

• Intuitive Graphical Interface

Microscope Start-up Kit

Recipe:• PXI Digital Framegrabber• LabVIEW and NI IMAQ

Vision• DVC filter wheel camera • Olympus BX61

Microscope and BX-UCB Microscope Controller Unit

Microscope Start-up Kit

• Easily Modified Software Architecture

• Open Source Code Based on LabVIEW Graphical Programming Environment

• Can be Upgraded Using Standard PCI or PXI-Based Technology

• Located on NI Developer Zone

Microscope Start-up Kit

• Login to Microscope and Camera Initialization

Microscope Start-up Kit

• Microscope Serial Control Through Controller Unit

Microscope Start-up Kit

• Set Objectives if changes in Front Panel

Microscope Start-up Kit

ApplicationsMonitoring and control for:• Microscope Functions

– Cell Counting– Cell Analysis– Cell Imaging

• Other Microscope Applications

Microscope Start-up Kit

• Close Camera and Microscope Session.

Microscope Start-up KitDisplayed: • Neuroscience Show

’02, ’03• Pittcon ‘03

Microscopy with National Instruments Products

Image courtesy of University of Utah

Example:• Quantitative Analysis of

Cytoskeletal Fiber Orientation• LabVIEW and IMAQ Vision

Pioneering new types of Image Processing Tools for Biomedical Research

Future Microscope Kits – Compact Vision SystemNI 1450 Series

Model 1454 1455

Ram/Flash 128/32 128/128

Speed (MIPS) 883 1436

Sensor Size You choose You choose

SoftwareConfigurable or programmable

Configurable or programmable

Digital I/O 29 DIO 29 DIO

Video out Yes Yes

Operating Temp. 0-55 C 0-55 C

• Stand-alone System with Embedded Analysis Functionality

• Based on ProvenLV RT Technology

• IEEE 1394.a acquisition – Supports any DCAM

compliant camera– 640 x 480 @ 100 fps– 1024 x 768 @ 15 fps

• Industrial ruggedness – No fan or external vents – Shock resistant

NI 1450 Series Connectivity

Configurable I/O10/100 Base T

RS-232

VGA Output

IEEE 1394.a

Digital I/O

Vision Builder for Automated Inspection – Configuration InterfaceMain Window Inspection StepsInteractive Help Inspection Diagram

Window

Vision Builder for Automated Inspection• Ease of Use

– Prototyping and configuration environment

• Fast Development– Deployable user

interface included– Decision-making capabilities– Digital and

Serial I/O capabilities

• Evaluation Software Available

• Generates LabVIEW code!

Thank You!

Questions?