Post on 23-Dec-2015
Fermentation Technology-> Fermentation: from latin -> ”fervere” -> to boil (describing the
anaerobic process of yeast producing CO2 on fruit extracts)
-> Nowadays: more broad meaning!!!!
The five major groups of commercially important fermentations:
-> Process that produces microbial cells (Biomass) as a product-> Process that produces microbial enzymes as a product-> Process that produces microbial metabolites (primary or secondary) as a
product-> Process that produces recombinant products (enzymes or metabolite) as a
product -> Process that modifies a compound that is added to the fermentation –
transformation process
Growth: basic concepts
Anabolism = biosynthesis
Catabolism = reactions to recover energy (often ATP)
Precursors
Regeneration of NAD+
FermentationRespiration
No added terminal e--acceptor Oxidant = terminal e--acceptor
ATP: substrate level phosphorylationATP: (e--transport) oxidative phosphoryl.
Glucose
2 Glyceraldehyde-3-P 2 ATP2 NADH
2 Pyruvate
2 Lactate+ 2 H+
Acetaldehyde+2 CO2
2 Ethanol
Acetate+ Formate
H2 + CO2
Glucose2 ATP2 NADH
2 Pyruvate
2 Acetyl-CoACO2
Citric acidcycle
CO2
GTPNADH, FADH
Cytoplasmic membrane
out
inATP
H+H+H+H+H+H+
O2H2O
1 Glucose 2 ATP 1 Glucose 38 ATPSlow growth/low biomass yield Fast growth/high biomass yield
Major functions of a fermentor
1) Provide operation free from contamination;
2) Maintain a specific temperature;
3) Provide adequate mixing and aeration;
4) Control the pH of the culture;
5) Allow monitoring and/or control of dissolved oxygen;
6) Allow feeding of nutrient solutions and reagents;
7) Provide access points for inoculation and sampling;
8) Minimize liquid loss from the vessel;
9) Facilitate the growth of a wide range of organisms.
(Allman A.R., 1999: Fermentation Microbiology and Biotechnology)
Microbial Growth Kinetics
• Fermentation can be carried out as:1. Batch2. Continuous3. Fed-batch processesMode of operation is to a large extent dictated
by the type of product being produced.
Growth cycle of yeast during beer
fermentation
From: Papazian C (1991), The New Complete Joy of Home Brewing.
Fermentation Technology
-> Process that produces microbial cells (Biomass) as a product mainly for -> baking industry (yeast) -> human or animal food (microbial cells)
Fermentation Technology
-> Process that produces microbial enzymes as a product
mainly for -> food industry
Fermentation Technology
-> Process that produces microbial metabolites (primary or secondary) as a product
Fermentation Technology
-> Process that produces microbial metabolites (primary or secondary) as a product
Fermentation Technology
-> Process that produces microbial metabolites (primary or secondary) as a product
Fermentation Technology
-> Process that produces microbial metabolites (primary or secondary) as a product
Fed-Batch CultureA fed-batch is a biotechnological batch process which is based on feeding of a growth limiting nutrient substrate to a culture. The fed-batch strategy is typically used in bio-industrial processes to reach a high cell density in the bioreactor. Mostly the feed solution is highly concentrated to avoid dilution of the bioreactor. The controlled addition of the nutrient directly affects the growth rate of the culture and allows to avoid overflow metabolism (formation of side metabolites, such as acetate for Escherichia coli, lactic acid in cell cultures, ethanol in Saccharomyces cerevisiae), oxygen limitation (anaerobiosis).
Batch culture versus continuous culture
Continuous systems: limited to single cell protein, ethanol
productions, and some forms of waste-water treatment
processes.
Batch cultivation: the dominant form of industrial usage due to its
many advantages.
(Smith J.E, 1998: Biotechnology)
Advantages of batch culture versus continuous culture
1) Products may be required only in a small quantities at any given time.
2) Market needs may be intermittent.3) Shelf-life of certain products is short.4) High product concentration is required in broth for optimizing
downstream processes.5) Some metabolic products are produced only during the stationary
phase of the growth cycle.6) Instability of some production strains require their regular
renewal.7) Compared to continuous processes, the technical requirements
for batch culture is much easier.