MEF: transfection of plasmid DNA · MEF: transfection of plasmid DNA Lipocalyx GmbH Weinbergweg 23...

VY-01LB-00, VY-01LB-01

Fig. 1: Microscopic observations of MEFs (mouse embryonic fibroblasts) transfected with Drp1-YFP, co-stained with mitotracker red and DAPI, by using Viromer® YELLOW

Overnight transfection 6-well plate format, standard protocol

Data from A. Goldman, Prof Atan Gross Lab, Weizmann Institute of Science (Israel)

MEF: transfection of plasmid DNA

Lipocalyx GmbH Weinbergweg 23 06120 Halle Germany viromer-transfection.com viromer-transfection.com

Fig. 2: Transfection of a plasmid coding for an outer mitochondrial membrane protein tagged with GFP in MEFs (mouse embryonic fibroblasts) using Viromer® RED

>> GFP signal co-localizes with mitotracker red CMXRos

Cell nuclei

Mitotracker red CMXRos

VR-01LB-00, VR-01LB-01

Fig. 3: 3D rendering of MEFs (mouse embryonic fibroblasts) transfected with a plasmid DNA coding for an outer mitochondrial membrane protein using Viromer® RED

>> GFP signal co-localizes with mitotracker red CMXRos

Mitotracker red CMXRos

VR-01LB-00, VR-01LB-01

Fig. 4: Expression of GFP in MEFs (mouse embryonic fibroblasts) after plasmid transfection with Viromer® RED

- Standard conditions in 24-well (500ng DNA/well)

Data from K. Devi Selvasaravanan, Stattorst’s Lab, Institute of Biochemistry,University of Tübingen (Germany)

Fig. 5: Transfection efficiency (% of GFP positive cells) and cellviability after transfection of MEFs (mouse embryonicfibroblasts) using Viromer® RED and Viromer® YELLOW

MAX: approx. 20% efficiency with Viromer® RED No toxicity

Anonymous data

VR-01LB-00, VR-01LB-01

MEF: transfection of plasmid DNA · MEF: transfection of plasmid DNA Lipocalyx GmbH Weinbergweg 23...

Documents

Transcript of MEF: transfection of plasmid DNA · MEF: transfection of plasmid DNA Lipocalyx GmbH Weinbergweg 23...

Protocol: Lipofectamine 3000 Transfection Reagent …assets.thermofisher.com/TFS-Assets/BID/Reference...Transfection protocol Transfection components Component Cat. No. Thermo Scientific

Plasmid Purification Reinvented2 • Fastest: Simple 20 minute Midi/Maxi preps. • Highest Yield: 6x more plasmid. • ™Ultra-Pure: EndoZero , vaccine grade*, and transfection ready.

transduction and transfection

Transfection of eukaryotic cells Bryon Anderson. Presentation Overview What is Transfection? Transfection vs. Transformation Purpose of Transfection How.

For preparation of transfection-grade plasmid DNA from E. coli

Infectious alphavirus production from a simple plasmid transfection

NucleoBond Xtra Midi/Maxi/EF - Macherey-Nagel AG · 20 µm Transfection of primary rat hippocampal neurons with NucleoBond® Xtra EF purified plasmid DNA Plasmid purification: 9 kb

Adenosine Receptor A1-A2a Heteromers Regulate EAAT2 ...downloads.hindawi.com/journals/ppar/2020/2410264.pdf · 2.6. EYFP-A1AR Plasmid, A2aAR siRNA, YY1 siRNA, and HDAC1 siRNA Transfection.

Transfection of naked DNA - Jagiellonian Universitybiotka.mol.uj.edu.pl/zbm/handouts/2011/gene...requires the eucaryotic promoter in a plasmid vector 1. Viral promoters : CMV, SV40

EGFR signaling confers resistance to BET inhibition in ...Origene Inc. (TL320326, Rockville, MD, USA). For plasmid transfection, Lipofectamine 2000 was used ac-cording to the standard

TRANSFECTION PROTOCOL Lipofectamine 3000 Transfection Reagent MCF10A · 2020. 8. 12. · TRANSFECTION PROTOCOL Lipofectamine 3000 Transfection Reagent MCF10A Breast cancer cells Complete

KLONING Techtumnytt.pdfPlasmid-DNA (transfection-grade) Art.nr Kit Pris nu MINI-PREPARATION 740490.50 NucleoSpin® Plasmid transfection‑grade (50 preps) 594:-740490.250 NucleoSpin®

Optimal Transfection Conditions and the Safety Profiles … · Optimal Transfection Conditions and the Safety Profiles of Dextran-Spermine/Plasmid DNA as a Potential Gene Transfer

TransIT X2® Dynamic Delivery System for RISPR/as9 Plasmid … · Table 1. Recommended starting conditions Transfection Optimization: Determine the best TransIT-X2®:DNA ratio for

HiSpeed™ Plasmid Purification · PDF fileProtocol for HiSpeed Plasmid Midi and Maxi Kits 14 ... Effectene , and SuperFect ® Transfection Reagents for high-efficency transfection

· Web viewFLuc, firefly luciferase. HEK293T cells were co-transfected with pCMV-luc-PDCD4 3’UTR and pRL-TK Renilla luciferase plasmid. Four hours after transfection, ...

Phosphate-enhanced transfection of cationic lipid ... · Biochimica et Biophysica Acta 1369 1998 320–334 . Phosphate-enhanced transfection of cationic lipid-complexed mRNA and plasmid

Transfection. What is transfection? Broadly defined, transfection is the process of artificially introducing nucleic acids (DNA or RNA) into cells, utilizing.

MN guide to plasmid purification - Techtum 3 JJNucleoBond ® Xtra Midi • NucleoBond Xtra Maxi Superior plasmid Midi and Maxi kits – transfection-grade XXHighest speed – 30 min

K4 Transfection System...K4® Transfection System Manual EN 0218 7 2.3 Optimization protocol of DNA transfection for 48-well-format Use a suitable reporter gene plasmid, e.g. pCMV-ßGal,