Post on 26-Mar-2020
Mark S. Nardone FAES/NIH
Director, Bio-Trac Program 301-496-8290
nardonem@mail.nih.gov One Cloister Court, Bldg 60, Rm 60
Bethesda, MD 20814-1460
What is Bio-Trac? • Hands-on Training Workshops in Targeted Areas of Molecular Biology • Taught by over 250 Active Researchers/Bench Scientists • Presented at a Graduate/Post-Graduate Level • Open to the Scientific Community • Average 3-5 Days in Length • Currently 56 Different Workshops Offered • Specialty Programs
Who Takes a Bio-Trac Workshop • NIH Researchers, Post Docs, Post Bacs, Fellows
– Lab Chiefs, Principle Investigators, Lab Technicians • FDA, USDA, USUHS, WRAIR, USAMRIID, CDC, US Patent Office, NIST • Academia
– Researchers, Teachers, Graduate & Undergraduate Students
• Private Industry – Presidents & V.P’s of Pharmaceuticals; Lab Chiefs – Bench Scientists – Technicians
• Over 16,000 Scientists Have Participated in a Bio-Trac Program
Where Are Bio-Trac Attendees Coming From
Why Take a Bio-Trac Workshop
• Effective Use of Time and Resources • Stay on Top of the Latest Developments in
Your Area of Research • Your Research Has Taken You into a New Field
of Study • Career Advancement • Budget Cuts – Having to do More With Less
Background Information
• Formally Established in the Fall of 1985 • Evolved Out of a Series of EEO Training
programs in the late 70s • Roland M. Nardone, Ph.D. – CUA
– Tissue Culture – Cell Line Authentication
• Drs. Lou Cohen, Earl Laurence and Freddie Brown (NIDDK)
Bio-Trac Workshops Specialty Training Programs
in Molecular Biology
Microscopy • Immunofluorescence and Confocal Microscopy • Digital Imaging in Microscopy
• Transgenic Technology
Microscopy
Microscopy
• Laser Capture Microdissection
Immunology
• Cellular Immunology • Immunocytochemistry • Immunochemistry and Monoclonal Antibody Production • Vaccines: Development and Evaluation of Efficacy
Proteomics & Genomics
• Proteomics: Principles and Methods • Clinical Proteomics and Biomarker Discovery • Genome Analysis • Protein-DNA Interactions: Gene Specific and
Epigenetic Regulation of Expression of the Mammalian Genome
• Protein Informatics • Epigenetics • Next Generation Sequencing • RNA Seq
Microarrays
• DNA Micro Arrays: Fabrication and Applications
• Advanced Microarray Analysis and Pathway Integration
GeneChip® CustomSeq™ Arrays
Cell Based Workshops • Animal and Human Cell Culture: Methods and
Applications • Cryobiology: Principles and Methods • Cell Line Identification and Authentication • Stem Cells
Stem Cell Based Workshops • Collaboration with the Center for
Regenerative Medicine Branch (CRM) at the NIH
• Stem Cells: Principles and Methods • Human Induced Pluripotent
Stem Cells (hiPSC); Differentiation to Neural Lineages
• Engineering with CRISPR, TALENs, and ZNFs • Making iPSCs From Blood • Making Cardiomyocytes from iPSCs • Stem Cell Industry Symposium
PCR Based Workshops
• PCR Methodology: Principles, Optimization and Applications
• Real Time and Quantitative PCR
Expression Analysis: siRNA & miRNA
• Small Interfering RNA (siRNA) and Functional Genomics
• miRNA: Tools and Technologies for the Quantitative and Functional Analysis in Mammalian Cells
In situ analysis of miRNA expression
Custom Designed Programs
• NIH Institutes (NCI, NIEHS, NINR, NIDDK, NINDS) • USDA • FDA • US Patent Office • Merk, UpJohn, Eli Lilly, Glaxo SmithKline,
Boehringer Mannheim…. • Colleges
“On The Road” Training Programs
• Custom Designed Training Workshops • Provide Training at Your Facility • Save Time, Travel and Money
Next Gen Sequencing Workshop at Colorado State University March 2014
Bio-Trac Laboratory Hosting
• Lab Safety Group • NCI Cancer Prevention Workshop • NIAMS – Stem Cell Workshops - Center for
Regenerative Medicine (NCRM) – Stemgent – StemCell
Bio-Trac Helpers Core Facilities
• Transgenic Core Facility • Angiogenesis Core Facility • Microarray Core Facility • Confocal Core Facility • LCM Core Facility
Private Industry
• Zeiss – Immunofluorescent Microscopes • Roche, ABI, Stratagene – Real Time PCR • BD - Flowcytometer • MMI, Zeiss Palm, Leica, MDS Arcturus – Laser
Capture Microdissection • Nikon, Zeiss – Microinjection Scopes
Specialty Programs • Minority Faculty Student Program • Provides biotechnology training to minority
students and faculty members • Participants from Historically Black Colleges
and Universities, Hispanic Serving Institutes and Indian Tribal Colleges or Universities
• Since the inception of the program in 1992, there have been 37 different MFSP workshops
• MFSP has provided over 480 students and 425 faculty members and touched over 90 institutions
• Participants gain insights and experience in relevant specialized areas of biotechnology through hands-on lab work and lectures
• Attendees also acquire information pertaining to research training opportunities at the NIH along with intramural and extramural funding opportunities
Summer Genetics Institute • Collaboration with the National Institute of Nursing Research
(NINR) • One month intensive research hands-on training program to
increase the research capability among graduate students and faculty
• Helps develop and expand the basis for clinical practice in genetics among clinicians
• Established in 2000 and has provided training to more than 230 nurses
• 130+ peer reviewed publications have come from SGI Participants
• 47 SGI graduates have received Federal funding for the research proposal they developed during the SGI program
Junior Scientist Training Program Established Fall 2010
• A quality training program at the NIH that bridges the gap between the laboratory skills acquired by biology graduates to the proficiency level necessary to be productive in a research laboratory setting
• 9 Month Program • 16 Laboratory Sessions • 23 Lecture Presentations • 6 Graduate Credits through the FAES
JSTP Results
• NIH Post Bacs, Post Docs, Fellows, Research Scientists
• Strengthen laboratory skills • Strengthen effectiveness as a researcher
• Recent College Graduates, 4th Year Biology Major • Strengthen graduate school application • Strengthen skill set for employment in the sciences • Strengthen internship application
JSTP Results Cont’d
• Acceptance into Graduate School • Acceptance into Internship Programs • Employment in the Sciences
What the Future Holds
• Expansion of JSTP in the USM System • New Courses
– iPSC in Cardiomyocytes – RNA-Seq – Super Resolution Microscopy
• New Laboratory Facilities (2016-17) – Two New Training Laboratories in Building 10
• Research Training for Medical Students – Working with Area Medical Schools
• Collaborations With Area Institutions
Thank you!