Ling V. Sun & Li Wang

Institute of Developmental Biology and Molecular Medicine , Fudan University, and Shanghai Center for Bioinformation Technology, China

PBmice and MP-PBmice: A database system of PiggyBac (PB) insertional mutations in mice and a subsystem for mapping the mutations onto the mouse genome with large-scale experimental data

Transposons and Insertional Mutations Transposons: Mobile Genetic Elements

Barbara McClintock

chromosome

Transposon

Gene基 因基 因Transposon

Transposon

Mutant Gene Tagged

InsertionalMutagenesis

Transgenesis

Transposon

Advantages of Insertional Mutations

• can produce easily tractable mutations

• can produce large number of mutants at low cost and high speed

PB(PiggyBac): a high efficient transposition system in

mammalsMoth piggyBac(Cary et al. Virology 1989)

Mammal PB system(Ding et al. Cell 2005) +

Act-PBasePB[RFP]

PBasePB end

Traditional Knock-out PB insertional mutagenesis

Reduce Cost & Reduce Cost & Improve EfficiencyImprove Efficiency

Mutant 1 Mutant 2

Mutant 3

PB

Breeding

Mutant

ES

Screen cell linesMicro-

injection IVF

Molecular cloning

PB: an ideal tool for insertional mutagenesis

http://www.idmshanghai.cn/PBmice

INSERT interface

MP-PBmice system (insertional mutations mapping system of PBmice

• Experimental Procedure

• Flow production

From PBmice to MP-PBmice

• Integrated information of PB insertional mutations and their characterizations in mice: PBmice (NAR, 2008 vol.36, Http://www.idmshanghai.cn/PBmice)

• Daily book-keeping and information exchange between experimental groups for the mapping procedure: MP-PBmice

• Design and implementation

• User Interfaces

• Features

MP-PBmice

• Design and implementation

• User Interface

• Features

Database Tables: the relational data

schema

•Access-control Table•Experimental Stage Table•Constant Table•Status Table

Model-View-Controller (MVC) Pattern & Struts-Spring-Hibernate (SSH)

Framework

MP-PBmice

• Design and implementation

• User Interfaces

• Features

viewDetail

detailed information page

comment box

MP-PBmice

• Design and implementation

• User Interface

• Features

Strict Access Control

Work Flow Control

Expandability

• Suitable for any genome

• Suitable for any experimental mapping method

Future Plan

• Improve PBmice Mapping Database

• Add Bioinformatics Tools Needed

Acknowledgments

Wenwei YangMin HanYuan ZhuangTian XuXiaohui WuYun Zheng

Ke JinJigang YangYang Zhong

Xing XieDongsheng LiNing Gu

For collaboration and further contact:Dr. Ling V. Sun (PI): Lingsun@fudan.edu.cn