Introduction to training

Agenda• Product overview

• What’s new from ProStar 325

• Hardware overview

• Initialization routine

• Software CD overview

• Detector installation & PolyVerfiy

• PolyDiagnostics

Product Overview

Based on the ProStar 325 with many shared parts

Two models available:

• Single 1024 array • Dual 1024 array

Control by both Star with PolyView 2000 and Galaxie(PolyView is now included with Star driver)

Product Overview - workstation control

Galaxie• Four 335 DADs per acquisition server

• Driver included in Varian LC control drivers

Star• two 335 DADs per Star Workstation

• Driver included in LC control option

• Installation of driver automatically installs PolyView 2000

Ethernet communications best with a switch (not a hub)

Product Overview

• Same cabinet• Same UV & visible lamps• Same flow cells• Same rear panel, connectors, interface boards & cables• Same communications protocol• Same power supply• Same Main pwb (difference in firmware)

ProStar 335 and 325 similarities

What’s New

Same look as ProStar 325 except no PPC capabilities

What’s New

Dual Array• 1024 photodiode sample and reference array

• Extended range spectra

• Used for Analytical to Prep Applications

Single Array• 1024 photodiode sample array

• 2 photodiode references (250 and 500 nm)

• No extended range

• Uses 9x0 and 4x0 flow cells only

Different optics module

Hardware Overview – optics module

Product Overview – ray trace diagram

Hardware Overview - Polychromator

Available slit widths

1nm2nm4nm8nm

16nm

Hardware Overview – dual path flowcell

Illustration is of a 9x1 flowcell

sample

reference

Hardware Overview – optics module

Wavelength calibration data must be entered into the EEPROM using Diagnostics software.

Electronics – wavelength calibration factors

Use the floppy disk to update the EEPROM

automatically

This label will be attached to the top of each optics module

Hardware Overview – Electronics

Only difference between 335 & 325 is firmware

Hardware Overview - Electronics

Add new MAC address label when swapping pwb’s

New MAC address label comes with each new PWB

Hardware Overview – initialization routine

• Peak search for 656.1nm deuterium emission line

• Determines dark current table (0%T)

• Gain (Lamp) calibration is now user driven. Stored in non-volatile memory.

Initialization

Status LED’sinitialized

not initialized

Hardware fault

Software CD overview

Software CD ships with each ProStar 335 detector.

Part number: 85 102114 00

Auto-loading CD which contains:• Galaxie drivers and manuals• Star driver and manuals• PolyVerfiy software• PolyDiagnostics

Software CD overview

Customer software CD 85-1xxxxx-00 will contain

Software CD overview – installation PolyVerify

Use PolyVerify to check the performance of the ProStar 335

After installing, a desktop icon will be created called

ProStar

Software CD overview - installation PolyVerify

Only two test are required at installation;

Noise/Drift Wavelength

accuracy

IQ/OQ is also availablepart number: 03-914756-00;4

Software CD overview – installation PolyVerify

Print customer certificate

The ProStar 335 must be initialized and have both lamps on for at least one hour prior to running any tests.

Noise test : pump water for at least 10 minutes at a pump head pressure of at least 100 atm using a back pressure restrictor of 40psi.

Wavelength accuracy: same conditions as the Noise test.

Software CD overview - PolyDiagnostics

Browse the CD using Windows Explorer.

Diagnostics is not self loading and not intended for

customer use.

Installing PolyDiagnostics

Software CD overview - PolyDiagnostics

To run PolyDiagnostics you will need to copy these three files to the ProStar directory where PolyVerify is installed. From there double click the “PolyDiagnostics.exe” file using Windows Explorer.

Software CD overview - PolyDiagnostics

Similar appearance to

325 Diagnostics

Software CD - PolyDiagnostics

Service folder also contains the firmware updater program

Use the Diagnostics program to update the EEPROM and perform various calibration routines i.e. wavelength & lamp calibrations

Software CD - PolyDiagnostics

The EEPROM contents will need updating whenever;

• the Main PWB is replaced• the optics module is replaced• to update installation & maintenance dates

Software CD - PolyDiagnostics

EEPROM contents;

• Detector serial number• MAC address• Wavelength calibration factors, slope and offset• IP address• Gateway address• Subnet mask• Lamp timer (on/off date and times)• Detector installation date• Last maintenance date• Error log• Hg wavelength calibration date

Software CD - PolyDiagnostics

When to calibrate

Performing a Lamp Calibration will help reduce noise and improve linearity.

• Deuteriunm lamp is changed• Visible lamp is changed• Every 1000 lamp source hours• Increasing signal noise• When changing flowcell to one of a different pathlength