BIGGEST REVOLUTION BY

THE SMALLEST SCIENCE

Welcome toNANO WORLD

Impact of Nano formulation in Horticultural crop Pest

Management V. G O W T H A M

2 0 1 6 6 0 0 5 0 4A G R L . E N T O M O L O G Y

TA M I L N A D U A G R I C U LT U R A L U N I V E R S I T Y

Nanotechnology…?

“Nanotechnology is the art and science of manuplating matter at nano scale’’

“Nanotechnology refers to controlling, building, and restructuring materials and devices on the scale of atoms and molecules”.

A nanometer (nm) is one-billionth of a meter.

Nanopesticides

Nano-scale either active ingredients with a particle size of 100 nm or less

Formulation of a pesticides Nano emulsionNano suspension Nano encapsulationNanoparticles

Nano Emulsion

Consist of lipid or polymeric vesicles or particles

Size 20-200nm

Larger surface area, slower release

Non sedimentation or creaming

Development of an insecticidal nanoemulsion with Manilkara subsericea (Sapotaceae) extract

Fernandes et al. 2014Journal of Nanobiotechnology 2014,

12:22

Hexane-soluble fraction from ethanolic crude extract from fruits of Manilkara subsericea and its triterpenes were considered active against a cotton pest (Dysdercus peruvianus).

poor water solubility, including triterpenes

A nano emulsion prepared 5% of oil (octyldodecyl myristate), 5% of surfactants (sorbitan

monooleate/polysorbate 80), 5% of apolar fraction from M. subsericea 85% of water.

Nano EncapsulationEncapsulation packaging the nano-scale

active ingredient within a kind of tiny “ envelope or shell”

Nanotechnology enables us to manipulate the properties of the outer shell of the capsule.

Uses

Protection

Increase solubility

Reduce the contact of active ingredients with agriculture workers

Environments reducing run off rates

Mohdzobir et al., 2011

Efficient Management of Fruit Pests by Pheromone Nanogels

Deepa Bhagat et al.,Scientific Reports | 3 : 1294 | DOI: 10.1038/srep012942013

Pheromones

• Nanogel prepared using methyl eugenol (ME)+low-molecular mass gelator

• Very stable at ambient condition• works well in rainy season• Last for month

(Deepa Bhagat,2013)

Slope magnitude comparison

(Deepa Bhagat,2013)

(Deepa Bhagat,2013)

30 days after storage

Fruit flies attracted towards the thin film nanogel

1 2

3 4Nanogel

Fruit fly counting

Establishment

(Deepa Bhagat,2013)

Field experiment

Num

ber o

f cat

ches

Number of days (Deepa Bhagat,2013)

Number of days of catches for the ME and Nano ME gel

Nano suspension

Submicron colloidal dispersion of pure active compounds typically range from 50-500nm.

Improvement of efficacy due to higher surface area.

Higher solubility, higher mobility.

Induction of systematic acivity due to smaller particle size.

Nanoparticles

Solid core or matrix, usually composed of metallic atom

Material used for the fabrication Metal oxide certain and silicates, magnetic material.

Biocompatible Chitosan Nanoparticles Incorporated Pesticidal Protein Beauvericin (CSNp-BV) Preparation for the Improved Pesticidal Activity Against Major Groundnut

Defoliator Spodoptera Litura (Fab.) (Lepidoptera; Noctuidae)

Arvind Bharani et al.,International Journal of ChemTech Research,2014

Cumulative mortality (%) of S.litura treated with free beauvericin (F-BV) and chitosan nanoparticles incorporated beauvericin (CSNp-BV)

(Arvind Bharani, 2014)

Effect of free beauvericin (F-BV) and chitosan nanoparticles incorporated beauvericin (CSNp-BV) on pupal and adult emergence

(Arvind Bharani, 2014)

DNA-tagged nano gold: A new tool for the control of the armyworm, Spodoptera

litura Fab. (Lepidoptera: Noctuidae)

Chakravarthy et al.,African Journal of Biotechnology Vol. 11(38), pp. 9295-9301, 10

May, 2012

4th days after treatment

5th days after treatment 6th days after treatment

3rd days after treatment

7th days after treatment

control

THANK YOU