Post on 25-Jul-2020
Hyperspectral imaging - Integration and application in a normothermic extracorporeal perfusion setup
F. Tetschke1, W. Markgraf1, S. Koch1, M. Gransow1, M. Janssen2, C. Thiele1, A. Kulcke3, H. Malberg1
1 Institute of Biomedical Engineering, TU Dresden, Dresden 2 Clinic of Urology and Children Urology, University Clinic Saarland, Homburg (Saar) 3 Spekled GmbH, Pepelow
florian.tetschke@tu-Dresden.de
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Motivation & Background Hyperspectral Imaging Experimental Setup Results Outlook
Motivation & Background
Motivation & Background
Normothermic Machine Perfusion:
• pre-conditioning of (marginal) organs
• assessment of organ condition (Hosgood et al.1)
• experimental: pharmaceutical studies / simulation
1 Hosgood et al., Ex vivo normothermic perfusion for quality assessment of marginal donor kidney transplants, British Journal of Surgery, 2015
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Motivation & Background Hyperspectral Imaging Experimental Setup Results Outlook
Camera based measurements
Contactless, noninvasive
Real time
Cost effective (no disposables)
Easy to integrate (NMP, laparoscopy)
www. gildenphotonics.com
Features
Spatial information
Spectral information
Spectroscopy
x
Imaging
x
Hyperspectral Imaging
Hyperspectral Imaging
Hyperspectral Imaging
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Motivation & Background Hyperspectral Imaging Experimental Setup Results Outlook Hyperspectral Imaging
x
y
ʎ = 500-1000nm
x
y
Hypercube
spectral axis
spatial axis
camera chip
spectrograph
entrance slit
lens
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Motivation & Background Hyperspectral Imaging Experimental Setup Results Outlook
Experimental Setup
Experimental Setup
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Motivation & Background Hyperspectral Imaging Experimental Setup Results Outlook
520 nm 570 nm 620 nm
...
870 nm 920 nm
... ...
Results
Results
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Motivation & Background Hyperspectral Imaging Experimental Setup Results Outlook
𝑂𝐷𝑅 =𝐴𝐸600 𝑛𝑚
𝐴𝐸569 𝑛𝑚
𝑂𝑥 =𝑂𝐷𝑅 − 𝑛
𝑚
Results
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Motivation & Background Hyperspectral Imaging Experimental Setup Results Outlook
flushed 5 min reperfusion 110 min reperfusion
Results
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Motivation & Background Hyperspectral Imaging Experimental Setup Results Outlook
26 x 26 x 26 mm
Novel sensor generation
• Fast measurements Real time perfusion
• High spatial resolution
Development of robust, multivariate Algorithms
• HB - HBO2
• fluorescence (ICG)
• water
• lipides
ʎ = 500-1000nm
www. bayspec.com
www. ximea.com
Outlook
Outlook
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Motivation & Background Hyperspectral Imaging Experimental Setup Results Outlook
Dr. med. Martin Janssen - UKS Dr. med. Ulf Aschenbrenner – UKD Dr. Axel Kulcke – Spekled GmbH
Thank you!
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Motivation & Background Hyperspectral Imaging Experimental Setup Results Outlook
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Motivation & Background Hyperspectral Imaging Experimental Setup Results Outlook
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Motivation & Background Hyperspectral Imaging Experimental Setup Results Outlook