Post on 16-Jun-2018
2015 LanzaTech. All rights reserved.
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GMOD powering LanzaTech
2016 GMOD Meeting
Indiana University, Bloomington, Indiana, United States
June 30 – July 1, 2016
Asela W. S. Dassanayake
Bioinformatics Research Scientist
Model Systems Group
asela.dassanayake@lanzatech.com
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• LanzaTech - Who we are and what we do
• How GMOD is powering the information flow at LanzaTech
• Proposing a Synthetic Biology platform?
The outline
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Ed Hawkins,Climate scientist,National Centre for Atmospheric Science,University of Reading.@ed_hawkins
65% of 2°carbon budget: USED
1870-2011: 1900 GtCO2
Remaining: 1000 GtCO2
Must stay in the ground
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Carbon smart world
Proprietary Acetogenic Biocatalyst
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Worlds’ first autotrophs
Acetogenic bacterium with ability to utilize gases as sole energy and carbon source – CO– CO+H2 or CO+CO2+H2
– CO2+H2
LanzaTech has developed a proprietary
strain of Clostridium autoethanogenum
Obtained by extensive selection program,
having improved characteristics– High gas uptake and ethanol production rates– Fast growth on defined minimal media– Non-sporulating and non-motile
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1 Organism, over 20 Products…
Pyruvate
CO/H2
Acetyl-CoA
Fatty Acids,
Terpenoids
Aromatics
Ethanol
Succinate
Lactate
2,3-Butanediol
(2,3-BDO)
Biodiesel (FAEE)
3-Hydroxypropionate
(3-HP)
Isopropanol
Acetone
3-Hydroxybutyrate
(3-HB)
1,3-Butanediol (1,3-BDO)
Partnerships
Butylene
1,3-Butadiene
Biopolymers
Branched-chain
Amino Acids
Butyrate
1-Butanol
Methyl Ethyl Ketone
(MEK)
2-Butanol
Acetoin
1,2-Propanediol
1-Propanol
Jet Fuel
Isoprene
Aromatics
DiscoveryContinuous
lab-scale
fermentation
Scaled
Up1 Organism, over 25 Products…
Acetate
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Piloting>50% selectivity
at lab scale
Gas Feed Stream
Gas Reception Compression Fermentation Recovery ProductTank
HydrocarbonFuels
Ethanol
Butanediol
Acrylonitrile
Butadiene
Styrene (ABS)
Nylon 6,6 (from Adiponitrile/HDMA)
PropanolButanol
The LanzaTech Process
Industrial Waste Gas
MSW WasteBiomass
We use:
Land Corn For People
We do not use:
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A sensible path
SugarCane
Strong Pipeline and Committed Partners
Commercial Production 2017
3 Commercial projects underway
ArcelorMittal, Gent
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Ground Works StartedOctober 2015
Gas Testing Station Produces Ethanol
January 2016
Connection to Steel Mill Gas Lines March 2016
1 Organism, over 20 Products…
Pyruvate
CO/H2
Acetyl-CoA
Fatty Acids,
Terpenoids
Aromatics
Ethanol
Succinate
Lactate
2,3-Butanediol
(2,3-BDO)
Biodiesel (FAEE)
3-Hydroxypropionate
(3-HP)
Isopropanol
Acetone
3-Hydroxybutyrate
(3-HB)
1,3-Butanediol (1,3-BDO)
Partnerships
Butylene
1,3-Butadiene
Biopolymers
Branched-chain
Amino Acids
Butyrate
1-Butanol
Methyl Ethyl Ketone
(MEK)
2-Butanol
Acetoin
1,2-Propanediol
1-Propanol
Jet Fuel
Isoprene
Aromatics
DiscoveryContinuous
lab-scale
fermentation
Scaled
Up1 Organism, over 25 Products…
Acetate
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Piloting>50% selectivity
at lab scale
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BIOENGINEERING
FERMENTATION
GENOMIC, TRANSCRIPTOMIC,
PROTEOMIC, METABOLOMIC DATA
BIOINFORMATICS, STATISTICS, MODELING
BIOCHEMISTRY, PATHWAY ANALYSIS
Information flow at LanzaTech
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GMOD at LanzaTech
External Collaborators
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Apollo at LanzaTech - Internal
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Transcriptional Regulator
Methyl accepting chemotaxis protein
Transcriptional Regulator
Uncharacterised ORF
Iron-Sulphur binding cluster
Roshomon
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Tuff-t
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Tuff-t
1 Organism, over 20 Products…
Pyruvate
CO/H2
Acetyl-CoA
Fatty Acids,
Terpenoids
Aromatics
Ethanol
Succinate
Lactate
2,3-Butanediol
(2,3-BDO)
Biodiesel (FAEE)
3-Hydroxypropionate
(3-HP)
Isopropanol
Acetone
3-Hydroxybutyrate
(3-HB)
1,3-Butanediol (1,3-BDO)
Partnerships
Butylene
1,3-Butadiene
Biopolymers
Branched-chain
Amino Acids
Butyrate
1-Butanol
Methyl Ethyl Ketone
(MEK)
2-Butanol
Acetoin
1,2-Propanediol
1-Propanol
Jet Fuel
Isoprene
Aromatics
DiscoveryContinuous
lab-scale
fermentation
Scaled
Up1 Organism, over 25 Products…
Acetate
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Piloting>50% selectivity
at lab scale
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• User data organization and management• BLAST Interface• Database Searching
• Literature searches and bibliography
• Genome browser
• Data Visualization
• Synthetic Biology CAD with SBML/SBOL
Proposing an Integrated Synthetic Biology platform
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Integrated Synthetic Biology platform
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More Users? Funding?