GEO—Gene Expression Omnibus

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GEO—Gene Expression Omnibus. IBGP705 TA: Lee Cooper February 18, 2009. Today’s lab. Complete tutorial Email answers to questions to TA by 6pm today. Today’s lab. Accessing GEO GEO Datasets GEO Profiles The “Help” Section BRB Array Tools. - PowerPoint PPT Presentation

Transcript of GEO—Gene Expression Omnibus

GEO—Gene Expression Omnibus

IBGP705

TA: Lee Cooper

February 18, 2009

Today’s lab

• Complete tutorial

• Email answers to questions to TA by 6pm today

Today’s lab

• Accessing GEO

• GEO Datasets

• GEO Profiles

• The “Help” Section

• BRB Array Tools

Preparing and carrying out a microarray experiment

Analysis of microarray data: clustering

Potential problems with microarray

Dealing with microarray problems

• Best answer = normalization

• Selection of normalization method/s

• Software

• Core lab

Today’s lab: GEO

• Various ways to visually examine microarray data to determine special need for normalization/ initial quality of data

• Accessing GEO

• GEO Datasets

• GEO Profiles

• The “Help” Section

• BRB Array Tools

Accessing GEO

GEO Datasets

GEO Datasets (old interface)

GEO Datasets (new interface)

Check distribution (normalization) and experimental design

Clustering (Algorithms and Parameters)

GEO Datasets: Analysis

GEO Profiles

GEO Profiles

GEO Profiles

GEO Profiles

GEO Profiles

BRB Array Tools