Post on 15-Jul-2020
GENE CLONIN GAND DNA ANALYSI SAn Introductio n
T.A . BROWN
Fourth Edition
Preface to the Fourth Edition, vi i
Preface to the Third Edition, ix
Preface to the Second Edition, x
Preface to the First Edition, xii
Part 1 : The Basic Principles of Gene Clonin gand DNA Analysis, 1
1 Why Gene Cloning and DNA Analysis are Important, 3
2 Vehicles for Gene Cloning : Plasmids and Bacteriophages, 1 4
3 Purification of DNA from Living Cells, 28
4 Manipulation of Purified DNA, 5 2
5 Introduction of DNA into Living Cells, 8 5
6 Cloning Vectors for E. coli, 104
7 Cloning Vectors for Eukaryotes, 13 0
8 How to Obtain a Clone of a Specific Gene, 156
9 The Polymerase Chain Reaction, 17 9
Part 2: The Applications of Gene Cloning an dDNA Analysis in Research, 195
10 Studying Gene Location and Structure, 19 7
11 Studying Gene Expression and Function, 21 7
12 Studying Genomes, 246
Part 3: The Applications of Gene Cloning an dDNA Analysis in Biotechnology, 269
13 Production of Protein from Cloned Genes, 271
14 Gene Cloning and DNA Analysis in Medicine, 293
15 Gene Cloning and DNA Analysis in Agriculture, 31 3
16 Gene Cloning and DNA Analysis in Forensic Science, 32 9
Glossary, 34 1
Index, 357