Post on 17-Dec-2015
Fluorescence Microscopy Study (pilot study)Fluorescence Microscopy Study (pilot study)
Study design:Study design: Study design:Study design: Individual pilot study. One forearm of a healthy 63-year old Individual pilot study. One forearm of a healthy 63-year old male volunteer received application of DermatoPoietinmale volunteer received application of DermatoPoietin® ® and another forearm received and another forearm received Placebo once-a-day for 28 Placebo once-a-day for 28 days. Fluorescence microscopy measurements were days. Fluorescence microscopy measurements were performed at baseline (day 0), day 7, 14, and 28.performed at baseline (day 0), day 7, 14, and 28.
StudyStudy performanceperformance: :
StudyStudy performanceperformance: :
Dr. Leonard Khiroug, Neurotar Ltd., Helsinki, Finland Dr. Leonard Khiroug, Neurotar Ltd., Helsinki, Finland
Purpose of Study:
Purpose of Study:
To assess the effect of DermatoPoietinTo assess the effect of DermatoPoietin® ® on collagen and on collagen and elastin deposition in skin by fluorescence microscopy using elastin deposition in skin by fluorescence microscopy using two-photon laser technique. two-photon laser technique.
Neurotar Ltd , Helsinki Finland www.neurotar.com United Technologies UT AG©
Fluorescence Microscopy Images Day 0 and Day Fluorescence Microscopy Images Day 0 and Day 2828
Neurotar Ltd , Helsinki, Finland United Technologies UT AG©
Red fibersRed fibers – Collagen; – Collagen; Green Green fibersfibers - Elastin - Elastin
Day 0Day 0
Day 0Day 0
Day 28Day 28
Day 28Day 28
0 50 100 150 2000
100
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Placebo DermatoPoietin
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en, a
.u.
Skin Depth, m0 50 100 150 200
0
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Placebo DermatoPoietin
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en, a
.u.
Skin Depth, m
DermatoPoietinDermatoPoietin® ® Replenishes Collagen / Elastin in Replenishes Collagen / Elastin in SkinSkin
Before (day 0)Before (day 0) After (day 28)After (day 28)
+ 80%+ 80%
RedRed – Collagen; – Collagen; GreenGreen - Elastin - Elastin RedRed – Collagen; – Collagen; GreenGreen - - ElastinElastin
Neurotar Ltd , Helsinki Finland www.neurotar.com United Technologies UT AG©
DermatoPoietin® (Day 0)
Neurotar Ltd , Helsinki, Finland
DermatoPoietin® (Day 28)
Neurotar Ltd , Helsinki , Finland
0 50 100 150 2000
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Day 0 Placebo DermatoPoietin
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en, a
.u.
Skin Depth, m
0 50 100 150 2000
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Day 28 Placebo DermatoPoietin
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en, a
.u.
Skin Depth, m0 50 100 150 200
0
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Placebo DermatoPoietin
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.u.
Skin Depth, m
Day 7
0 50 100 150 2000
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Day 14 Placebo DermatoPoietin
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en, a
.u.
Skin Depth, m
Time Course of DermatoPoietinTime Course of DermatoPoietin®® Effect on Effect on Collagen Collagen
Day 0Day 0 Day 7Day 7 Day 14Day 14 Day 28Day 28
The effect on collagen replenishment in aged skin is observed as early as 7 days after start of treatment with DermatoPoietin® and reaches a
maximum after about 2 weeks.
Neurotar Ltd , Helsinki, Finland United Technologies UT AG©
RedRed – DermatoPoietin – DermatoPoietin®® ; ; BlueBlue – Placebo – Placebo
Fluorescence Microscopy: ConclusionsFluorescence Microscopy: Conclusions
Application of DermatoPoietin® for 28 days increases the amount of Application of DermatoPoietin® for 28 days increases the amount of collagen in aged skin by 83% as compared to Placebo. collagen in aged skin by 83% as compared to Placebo.
Neurotar Ltd , Helsinki Finland www.neurotar.com United Technologies UT AG©
The effect is fast. Changes in skin collagen are observed as early as 7 The effect is fast. Changes in skin collagen are observed as early as 7 days after start of treatment. days after start of treatment.
The increase of collagen is accompanied by a coordinated increase in The increase of collagen is accompanied by a coordinated increase in elastin fibers. Collagen and elastin fibers form a well-organized network. elastin fibers. Collagen and elastin fibers form a well-organized network.
Fluorescence microscopy provides an excellent tool for monitoring the Fluorescence microscopy provides an excellent tool for monitoring the dynamics of structural changes in aging skin upon DermatoPoietin® dynamics of structural changes in aging skin upon DermatoPoietin® application.application.