Post on 28-Aug-2018
Fantastic Properties of Nano-bubbles
Kaneo Chiba Masayoshi Takahashi
Shrinking
Disappear
Microbubble Nano‐bubble
Continue
Ordinary bubble (Macrobubble)
Goes up rapidly
Burst at the surface
3
Microbubble
Collapse
Physical stimulus
Nano-bubble production by collapsing microbubble
Radical generation
・OH
Decomposition of organic chemicals
Waste water treatment
Physical stimulus
Nanobubble production
Collapse
Microbubble
with salt (electrolytes)
Nano-bubble
・OH
4
Nono-bubble generator
MicrobubbleNano-bubble
Physical stimulus
5
Analysis of nano-bubble by Dynamic light scattering (DLS)
0
2
4
6
8
10
12
14
16
18
20
76 93 113 138 168 204 249 304 370
Particle size (nm)
6
Microbubble 10 ~30μm
Nano-bubble< 200nm
The deference of size between MB and NB
7
Shrinking
Disappear
Several minutes Several months
Microbubble Nanobubble
Continue
The deference of life time between MB and NB
Why ? 8
Movement of microbubbles in electrophoresis cell
Light
Microscope
Electrode
Electrophoresis cell Water + microbubble
Electrode ( + ↔ − ) (− ↔ +)
9
time
ζpo
tent
ial(
mV
)
Change in ζ potential of microbubble over time
Diameter (μm)
0 5 10 15 20 25 30-20
-30
-40
-50
-60
-70
-80
Accumulation of surrounding ions
Ions Microbubble
time Ions
10
with salt (electrolytes)
Aqueous solution
Nano-bubble
+
+ +
+
+
+
+--
--
---
-
+
+
+
+
+
-
+
-
+
+
+
+
+
+ +
Stabilization mechanism of nanobubble (assumption)
Negatively charged
Surrounded by accumulated ions(counter ions)
Prevention of gas dispersion
Salting out
11
Nano-bubble generator
12
Normal ozone water vs. Ozone nano-bubble
Ozone water ; disinfection & sterilizing (Food industry, medical )
1 hour
100 days
Bubbling
Micro-bubble Nano-bubble
0.1mg/L
1.5mg/L
1.5mg/L
More than 1.0mg/L
Ozone
Normal method
Nano-bubble
Collapse
13
Oyster
Norovirus
Norovirus symptoms:vomiting, diarrhea, a low-grade fever, chills, headache, muscle aches
During cultivation
How to treat the problem of norovirusHow to treat the problem of norovirus
14
Chlorine ( high concentration )----- not good for foods
Chlorine
Norovirus
Conventional method
Oyster
Ozone water Oyster Ozone = strong oxidation ability
↓Virus → inactivation Norovirus 15
Oysters in ozone nanobuble water for 1 day
16
Before ozone nanobubble treatment
17
More than 99% of feline calicivirus in the body --- inactivated
After ozone nanobubble treatment
18
Practical method for inactivation of norovirus by ozone nano-bubble
19
Carps : recovering from Carp herpes ( serious disease )
Active effect for fishes
20
Oxidation ability of ozone nano-bubble as a form of mist 21
more than 80% of original oxidation ability
reduction of smell and bacteria from atmospheres 22
Oxygen nanobubble
Collapse of oxygen microbubble About 1% salt Recovery of weakened fishes
Active effect for creatures
23
Nano-Bubble Aquarium 24
Orchid in oxygen NB water for more than 1 month
Astonishing effects on plant
Still alive
25
Tap water Oxygen nano-bubble water
White radish Broccoli Broccoli
White radish
Initial growth of plants
26
Active effect for oysters
-20℃ for 1 day in oxygen NB
Room temperature
After 1 hour
Still alive
27
control TNFalpha
With oxygen nanobubble
control TNF alpha
Prevention of arteriosclerosis (hardening of blood vessel )
Oxygen nanobubble inhibits mRNA expression induced by TNFα in RAOECs
ICAM1
VCAM1
GAPDH
Department of Cardiology,Jichi Medical University
Application of nanobubble in medicine
Culture aorta endothelium of rat Cytokine stimulation(TNF alpha)
Heart attack
Stroke
28
Vagus nerve of rat : after 1 week
Isotonic NaCl solution Oxygen nanobubble
Tokyo Medical and Dental University
Application of nanobubble in medicine
Preservation of living tissue / organ
29
30
Thank you very much