Fabrication of an electrospun nanofibrous scaffold for use in the field

of tissue engineering

By: Shannon Daily & Tyler Crawford

Purpose To create a polycaprolactone mesh

which enables cell activity and seeks to eventually provide an application in the field of tissue engineering toward biomimetic skin graft.

Progress since last meeting Spun 4 more meshes

Visited Janelia Farm and used SEM

Changed procedure slightly

Created Timeline

Electrospun Meshes All 15 wt. % solution using acetic acid

as solvent 15 kV 15cm Used syringe pump (flow rate .02 ml/s)

for first three meshes› Now using pipette to reduce beading

SEM ImagesMesh 1, Image aMagnification: 148.685 µm x 111.514 µm

SEM ImagesMesh 1, Image bMagnification: 11.643 µm x 8.732 µm

SEM ImagesMesh 2, Image aMagnification: 173.875 µm x 130.406 µm

SEM ImagesMesh 2, Image bMagnification: 24.607 µm x 18.455 µm

To be worked on: View SEM images of new PCL meshes

› Visiting Janelia Farm on March 9 Begin varying voltage of pure PCL Create chitosan/PCL solution Begin cell work Updates

› ED› wikipage

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