Kristy D. Wendt

Material Surface Characterization: Partnership Building and Characterization Experience

Nano Research Facility Core Laboratories

Fabrication Laboratory

Particle Technology Laboratory

Surface Characterization Laboratory

Bio-Imaging Laboratory

FEI NovaTM 230 FE NanoSEM (EDS) Elemental Mapping

FEI TecnaiTM Spirit TEM

Leica UC7 Ultramicrotome

Veeco NanomanTM AFM

Zeiss LSM 710 Confocal Microscope

Starting On Campus: Training Protocol Development EM Core Facility Collaboration

Image and Technique Portfolio: Image Processing FEI Tecnai SpiritTM Quantifying Size Dispersion

Client: Dr. Younan Xia, Washington University

Sample: ”Bottom up” shape controlled synthesis silver and gold nanoparticles

Expectations: Quantification of size dispersion

500 nm 500 nm

TEM of ~200 nm Ag Nanocubes Binary Image with Global Threshold

Client: Dr. Brent Riggs, NRF

Sample: Photolithography, Fabrication

Expectations: Measure the thickness, density, and composition of films, identify profile shape, identify contamination and trace impurities

Image and Technique Portfolio: FEI NovaNanoTM SEM Finding the Right Angle

Image and Technique Portfolio: FEI NovaNanoTM SEM Troubleshooting Difficult Samples

Client: Dr. Pratim Biswas, Washington University

Sample: TiO2 crystal films

Expectations: Difficult to image sample, high-contrast/charging

Solution: Sputter coating current, reduced voltage, immersion mode, increased WD

Image and Technique Portfolio: FEI Tecnai SpiritTM TEM Surface Characterization

Client: Dr. Akira Ohnuma, Washington University

Sample: Asymmetric hybrid colloidal particles by precipitation polymerization

Expectations: Characterize a surface modified polystyrene bead

Image and Technique Portfolio: FEI NovaNanoTM SEM Murine Kidney Glomerulus

Client: Dr. Jeff Miner, Washington University

Sample: Kidney organogenesis in murine models

Expectations: Processing protocol development, basement membrane preservation

Image and Technique Portfolio: FEI NovaNanoTM SEM Aluminum Polarization Filters

Client: Dr. Victor Gruev, Washington University

Representative Research Area: Nanofabrication of aluminum polarization filters

Expectations: Side wall analysis

Image and Technique Portfolio: FEI Tecnai SpiritTM TEM Instrument Limit

Client: Dr. Byungkwon Lim, Washington University

Sample: Palladium-platinum bimetallic nanocrystals

Expectations: Ultrahigh magnification, show dendritic morphology; enhanced activity toward oxygen reduction in the fuel cell industry.

Image and Technique Portfolio: Efficient Sessions for Large Group Projects

Client: Dr. Kathy Hafer, Washington University

Sample: lambda phage

Expectations: Provide large scale learning experiment, including processing and imaging for lambda phage

Image and Technique Portfolio: Nanotoxicity in Yeast

Client: Dr. Yujie Xiong, Mark Brunson (REU) Washington University

Sample: Saccharomyces cerevisiae

Expectations: Processing protocol development for saccharomyces cerevisiae

Image and Technique Portfolio: FEI NovaNanoTM SEM Silica Chips on Silicon Substrate

Client: Dr. Lan Yang, Lina He Washington University

Sample: Silica microresinators on silicon substrates

Expectations: Lower contrast image on difficult sample, ETD, longer working distance

Image and Technique Portfolio: Qualitative Analysis by EDS Elemental Mapping/Overlay

Secondary Electrons Platinum Overlay 1 µm

Tin balls Overlay

Image and Technique Portfolio: Line Mapping Ag and Pt Nanoparticles

Silver Nanoparticles on Carbon

Gold on Carbon Platinum

Image and Technique Portfolio: Nanoskiving

Conventional Top Down Approach

Useful Versatile Highly Developed

High capital Operating costs No non-planar surfaces Material class restrictions

Conventional to Unconventional Techniques: Conventional Processing of Biological Tissue

High Contrast -Osmium tetroxide -Lead Citrate -Uranyl Acetate

Thin -Resin Infiltration -Thermal Polymerization -Section 65 nm

Dry -Ethanol Gradient

Fixation

Image and Technique Portfolio: Nanoskiving

PDMS EPOXY

EPOXY

PDMS

EPOXY

Cast and cure epoxy

Separate epoxy

EPOXY

Deposit metal

Embed in epoxy and cure

EPOXY

Ultramicrotomy

Thin-section ~70nm

Knife blade

Image and Technique Portfolio: Nanoskiving

Epoxy

Au on epoxy

Epoxy

PDMS

Knife

Xu Q et al. Acc. Chem. Res., 2008, 41 (12), pp 1566–1577

Image and Technique Portfolio: Nanoskiving

Xu Q et al. Acc. Chem. Res., 2008, 41 (12), pp 1566–1577

1. Nanostructure manipulation 2. 3D Stacking 3. No clean room

Collaborating with Nanoscale Informal Science Education Network Participating in WU’s Educational Research Group Organizing and Hosting Public Outreach Festivals at WU

Developing Outreach: Nanodays at the Saint Louis Science Museum

Managing Research Experience for Undergraduates (REU) Program

Kristy D. Wendt

-Broad range of material and biological EM processing -Enjoys addressing unique challenges

-Novel protocol development -Fabrication and particle analysis -Training and partnership building

-Works well with a broad range of clients to get beautiful results