Elizabeth M. Whitley, DVM, PhD, Dipl. ACVP1

Isabel Hurtado Rodriguez, DVM, MS, Dipl. ACVIM2

Amelia S. Munsterman, DVM, MS, Dipl. ACVS2

1Department of Pathobiology2Department of Clinical SciencesCollege of Veterinary MedicineAuburn University

www.sogonow.com/archives/2007/01/07-week/

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

5-year-old, male castrated, American quarter horse

Used for roping Recent, intermittent reluctance to work

on the right handRecent, unexplained weight loss

www.sogonow.com/archives/2007/01/07-week/

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Presented for colic that was non-responsive to Banamine and sedation

Clinical signs: Dehydration, tachycardia, tachypnea Copious nasogastric reflux when tubed Distended small intestine on ultrasound

and palpation Diagnosed a renal mass on ultrasound

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Rare neoplasm in animals other than pigs or chickens

Usually observed in young animals

“Wilms’ tumor” in children

http://www.nlm.nih.gov/medlineplus/ency/imagepages/9056.htm

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Three different morphologies Blastemal (undifferentiated, embryonic)

cells Epithelial cells Mesenchymal cells

Benign or malignant

en.wikibooks.org/.../Pregnancy_and_birth

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Vimentin Desmin

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Smooth muscle actin

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Cytokeratin

Cytokeratin

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Basement membrane

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Malignant nephroblastoma, triphasic, kidney, with metastasis to liver, spleen, and mesentery.

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Inactivation of the Wilms’ tumor gene (WT1) WT1 protein is a transcription factor that

functions as a tumor suppressor gene▪ Is expressed during renal and gonadal

development Defects in WT1 gene prevents normal

differentiation of metanephric stem cells Other genetic defects identified in

children with Wilms’ tumor ( including WT2, WT3, WT4)

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Thanks to Dr. Eva Sartin, Beth Landreth, Uschi Wolfert for technical assistance in histopathology.

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only