Post on 18-Jan-2018
description
Diolistics:Pun within Pun
Jeff GauthierComputational Neurobiology (PhD)
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First: bio + ballistics = biolistics(coined by Ed and Marlene Wolf, 1986)
1-2 µm tungsten beads,coated with DNA or RNA
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Propelled at tissue usingpressurized helium
What if we use dye instead of DNA?Lipophilic dyes DiA, DiD, DiI, DiO, …
Second: DiO + Biolistics = Diolistics(Gan, et al. 2000)
Gan et al, 2000
Mouse Retinal Ganglion Cellsfrom Sun, et al. 2002
Diffuses throughout most neurons (~200 µm diameter) in 30 min
Does not affect cell health (apparently)
Requires hours to express
Can severely alter cell metabolism
BiolisticsDiolistics
Multicolor “DiOlistic” Labeling of the Nervous System Using
Lipophilic Dye Combinations Gan, et al. 2000
Large-Scale Morphological Survey of Mouse Retinal Ganglion Cells
Sun, et al. 2002
Large-Scale Morphological Survey of Rat Retinal Ganglion Cells
Sun, et al. 2002
Quantitative morphologic classification of layer 5 neurons from
mouse primary visual cortexTsiola, et al. 2003
High-throughput Morphometric Analysis of Individual Neurons
Wu, et al. 2004
20002001
20022003
2004
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Gauthier 2004
(personal communication)
guinea pig retina