1Challenge the future

Development of fungal bioreactors for Treatment of Lignin/Humics- rich wastewater and sludge

Mostafa Zahmatkesh, CiTG, Water Management Department, Sanitary Eng. GroupVakantiecursus, TUDelft, January 2014

2Challenge the future

Project Outline

Wastewater Treatment EffluentS

lud

ge

Humics

Lignin

3Challenge the future

Recalcitrant Compounds

Lignin Humics

4Challenge the future

White rot fungi

Lignin Degrading Enzymes

Lignin Degradation

Lignocellulose Degradation

Lignin

Cellulose

Hemicellulose Lignin Degrading Enzymes

5Challenge the future

Challenge: Non-sterile conditions!

6Challenge the future

Approach

• Fungal Strain Prescreening

• Inoculation Method

• Bioreactor

• Process design

7Challenge the future

Challenges:

• Limiting bacterial growth and increasing the fungal

growth

• Inoculation strategy in continuous mode

• Maintaining short HRT and long fungal SRT in continues

run with optimum degradation activity