Post on 01-Feb-2018
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LAMPIRAN
LAMPIRAN I – ETHICAL CLEARANCE
63
LAMPIRAN II – PERMOHONAN IZIN PENELITIAN
64
LL L
LAMPIRAN III – PERSETUJUAN IZIN PENELITIAN
65
LAMPIRAN IV – PROSEDUR PEMBUATAN EKSTRAK JINTAN HITAM
(METODE SOXHLETASI)
Alat:
1. Soxhlet komplit
2. Cawan porselin
3. Pipet tetes
4. Beaker glass
5. Gelas ukur
6. Statip
7. Corong
8. Kertas saring
9. Neraca analitik
10. Water bath
11. Kompor listrik
12. Panci
Bahan:
1. Biji jintan
2. Pelarut etanol 70%
3. Akuades
Cara Kerja:
1. Biji jintan dikeringkan, kemudian ditumbuk sampai halus
2. Biji jintan yang telah ditumbuk ditimbang ± 200 gram (sesuai kapasitas
tabung), dibungkus dengan kertas saring dan dibentuk menyerupai tabung
sesuai besarnya diameter tabung tersebut
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3. Kertas saring berisi jintan hitam dimasukkan ke dalam extraction chamber,
kemudian ditambah pelarut 200 ml
4. Labu alas bulat diisi pelarut 500 ml, kemudian dihubungkan dengan
extraction chamber yang telah dipasang pada statip
5. Sisi bawah labu alas bulat dipasang alat pemanas (kompor) dan panci yang
berisi air
6. Kran pada kondensor dipasang selang dan dihubungkan pada air mengalir,
air dibiarkan mengalir selama proses ekstraksi
7. Pemanas (kompor) dinyalakan kurang lebih enam jam
8. Hasil ekstrak dalam labu alas bulat yang masih bercampur dengan pelarut
dan air diambil, dituangkan dalam cawan porselin, lalu diuapkan diatas
water bath dengan suhu maksimal 70° atau diangin-anginkan sampai
pelarut dan airnya hilang dan didapatkan ekstraknya
9. Prosedur ini diulangi beberapa kali hingga didapatkan ekstrak yang
diinginkan.
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LAMPIRAN V – HASIL UJI LABORATORIUM
No. Kode
sampel Kadar kolesterol
LDL
presipitan
Kadar LDL = kadar
kolesterol – kadar
LDL
1 N1 60,5 32,1 28,4 mg/dl
2 N2 65 39,2 25,8 mg/dl
3 N3 62,7 35,4 27,3 mg/dl
4 N4 44,4 22,6 21,8 mg/dl
5 N5 58,3 32,8 25,5 mg/dl
6 N6 50,5 28,1 22,4 mg/dl
7 R1 60,8 33,6 27,2 mg/dl
8 R2 53,3 29,2 24,1 mg/dl
9 R3 50 26,8 23,2 mg/dl
10 R4 60,7 34 26,7 mg/dl
11 R5 72,5 39,8 32,7 mg/dl
12 R6 52,1 26,4 25,7 mg/dl
13 J1 53 32,3 20,7 mg/dl
14 J2 54 31,9 22,1 mg/dl
15 J3 55,4 30,2 25,2 mg/dl
16 J4 51,4 25,8 25,6 mg/dl
17 J5 53,7 28,1 25,6 mg/dl
18 J6 55,1 31,6 23,5 mg/dl
19 S1 52,1 29,1 23 mg/dl
20 S2 75 46,7 28,3 mg/dl
21 S3 60,5 33,7 26,8 mg/dl
22 S4 58,7 29,5 29,2 mg/dl
23 S5 62 34,1 27,9 mg/dl
24 S6 56,5 31,2 25,3 mg/dl
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LAMPIRAN VI – HASIL ANALISIS (OUTPUT PROGRAM SPSS)
1. Jumlah sampel tiap kelompok
2. Analisis deskriptif pengaruh pemberian ekstrak jintan hitam terhadap
kadar kolesterol LDL tikus yang terpapar asap rokok
Descriptives
Kelompok Statistic Std. Error
Kadar LDL Kontrol Mean 25.200 1.0724
95% Confidence Interval for Mean
Lower Bound 22.443 Upper Bound 27.957
5% Trimmed Mean 25.211 Median 25.650 Variance 6.900 Std. Deviation 2.6268 Minimum 21.8 Maximum 28.4 Range 6.6 Interquartile Range 5.3 Skewness -.314 .845
Kurtosis -1.552 1.741
Rokok Mean 26.600 1.3687
95% Confidence Interval for Mean
Lower Bound 23.082 Upper Bound 30.118
5% Trimmed Mean 26.450 Median 26.200 Variance 11.240 Std. Deviation 3.3526 Minimum 23.2
Case Processing Summary
Kelompok
Cases
Valid Missing Total
N Percent N Percent N Percent
Kadar LDL Kontrol 6 100.0% 0 0.0% 6 100.0%
Rokok 6 100.0% 0 0.0% 6 100.0%
Rokok + Jintan 6 100.0% 0 0.0% 6 100.0%
Rokok + Simvastatin 6 100.0% 0 0.0% 6 100.0%
69
Maximum 32.7 Range 9.5 Interquartile Range 4.7 Skewness 1.366 .845
Kurtosis 2.383 1.741
Rokok + Jintan Mean 23.783 .8372
95% Confidence Interval for Mean
Lower Bound 21.631 Upper Bound 25.935
5% Trimmed Mean 23.854 Median 24.350 Variance 4.206 Std. Deviation 2.0508 Minimum 20.7 Maximum 25.6 Range 4.9 Interquartile Range 3.9 Skewness -.670 .845
Kurtosis -1.334 1.741
Rokok + Simvastatin Mean 26.750 .9291
95% Confidence Interval for Mean
Lower Bound 24.362 Upper Bound 29.138
5% Trimmed Mean 26.822 Median 27.350 Variance 5.179 Std. Deviation 2.2757 Minimum 23.0 Maximum 29.2 Range 6.2 Interquartile Range 3.8 Skewness -.912 .845
Kurtosis .163 1.741
70
3. Hasil uji normalitas data pengaruh pemberian ekstrak jintan hitam
terhadap kadar kolesterol LDL tikus yang terpapar asap rokok
Tests of Normality
Kelompok
Kolmogorov-Smirnova Shapiro-Wilk
Statistic df Sig. Statistic df Sig.
Kadar LDL Kontrol .212 6 .200* .924 6 .533
Rokok .262 6 .200* .889 6 .311
Rokok + Jintan .255 6 .200* .872 6 .236
Rokok + Simvastatin .193 6 .200* .937 6 .635
*. This is a lower bound of the true significance. a. Lilliefors Significance Correction
4. Hasil uji homogenitas varians dan uji parametrik One Way Anova
Oneway
Test of Homogeneity of Variances
Kadar LDL Levene Statistic df1 df2 Sig.
.201 3 20 .895
ANOVA
Kadar LDL
Sum of Squares df Mean Square F Sig.
Between Groups 34.690 3 11.563 1.680 .203 Within Groups 137.623 20 6.881 Total 172.313 23
71
LAMPIRAN VII – DOKUMENTASI PENELITIAN
Proses pemaparan asap rokok pada tikus Sprague Dawley
Proses anestesi dan pengambilan darah tikus
Sprague Dawley melalui pleksus retroorbita
72
Ekstraksi biji jintan hitam
dengan metode Soxhletasi
Proses pemeriksaan kadar
kolesterol LDL serum tikus
Sprague Dawley
73
LAMPIRAN VIII – BIODATA MAHASISWA
Identitas
Nama : Lalita Khairunnisa
NIM : 22010112130104
Tempat/tanggal lahir : Jakarta, 15 Oktober 1994
Jenis kelamin : Perempuan
Alamat : Citra Gran blok I8/3, Jatisampurna, Bekasi
Nomor telepon : 02184311296
Nomor HP : 081319300559
E-mail : lalita.khairunnisa@yahoo.co.id
Riwayat Pendidikan Formal
1. SD : SD Cindera Mata Bekasi Lulus tahun: 2006
2. SMP : SMP Negeri 1 Cibinong Lulus tahun: 2009
3. SMA : SMA Negeri 3 Bogor Lulus tahun: 2012
4. FK Undip : Masuk tahun: 2012
74
Keanggotaan Organisasi
1. Anggota Paduan Suara Mahasiswa (PSM) Universitas Diponegoro tahun
2012
2. Anggota Divisi Eksterna Badan Kelengkapan Reproductive Health
Education Unit (RHEU) FK Undip tahun 2013
3. Anggota Sie. Acara Try Out FK Undip tahun 2013
4. Anggota Sie. Konsumsi Gebyar Ramadhan tahun 2013
5. Reporter Buletin Communication Medical FK Undip tahun 2013-2014
6. Ketua Badan Kelengkapan Reproductive Health Education Unit (RHEU)
FK Undip masa jabatan tahun 2014-2015
7. Asisten Dosen Biokimia FK Undip masa jabatan 2014-2015
8. Anggota Sie. Acara Scientific Fair FK Undip tahun 2014
9. Anggota Paduan Suara Mahasiswa (PSM) Kedokteran Umum Undip tahun
2014-2015
10. Tim Penyusun Badan Semi Otonom (BSO) Jurnalistik FK Undip tahun
2014
11. Anggota Sie. Acara Porseni FK Undip tahun 2014
12. Sekretaris Umum BSO Lembaga Pers Mahasiswa (LPM) Impuls FK Undip
masa jabatan 2015-2016
13. Steering Committee Badan Kelengkapan Reproductive Health Education
Unit (RHEU) FK Undip masa jabatan tahun 2015-2016
14. Asisten Dosen Biokimia FK Undip masa jabatan 2015-2016
15. Anggota Sie. Pengabdian Masyarakat Kemah Bakti FK Undip tahun 2015
75
16. Konselor BSO Lembaga Pers Mahasiswa (LPM) Impuls FK Undip masa
jabatan 2016-2017