Post on 10-Sep-2018
CHROMATOGRAPHY
Think about foodCare about safety
wwwmn-netcom
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MACHEREY-NAGEL
EN ISO 9001 2008ZERTIFIZIERT
USAMACHEREY-NAGEL IncTel +1 484 821 0984Fax +1 484 821 1272E-Mail sales-usmn-netcom
Vol 7Pesticides
infomn-netcom wwwmn-netcom2 3
Focus on pesticides The global production and trading of food and feed products makes monitoring and control difficult
Only analysis of food and raw materials for the production of food can ensure safe and edible food Food safety is an essential part of our quality of life
MN shows examples for food analysis that will help you with your work and make our food safer
Vol 7 Pesticides in foodPesticides are chemical agents used for prevention and control of various pests Pesticides can be classified in herbicides (to control weeds) insecticides (to control in-sects) fungicides (to control molds and fungi) and roden-ticides (to control rodents) [EPA ndash US Environmental Protec-tion Agency ndash httpwwwepagovpesticidesfood]
Most commonly used are
bullOrganophosphates
bullCarbamates
bullPyrethroids
bullSulfonylurea
bullGlyphosate
bullOrganochlorine compounds
Since pesticides can be harmful to health of humans an-imals or the environment they need to be monitored and very responsibly used [EPA ndash US Environmental Protection Agency ndash httpwwwepagovpesticideshealthhumanhtm]
Among other authorities the US Environmental Protec-tion Agency (EPA) sets limits on how much of a pesticide can be used on food during the growing and processing and how much is allowed to remain in the food [EPA ndash US Environmental Protection Agency ndash httpwwwepagovpesti-cidessafetyapplicatorsrestricthtm]
infomn-netcom wwwmn-netcom2 3
Compounds of interestChemical structures of selected pesticides
Dioxathion Dimethoate Glyphosate Chlorpyriphos Chlorfenvinphos
PP
OC2H5H5C2O
OC2H5H5C2O
SS
SS
OO
PH3CO
OCH3
SSN
H
OH3C
OOP
HN OHHO
HO
PH5C2O
OC2H5
SO
N
Cl
Cl
Cl
Cl
Cl
O
OPO O
Cl
CH3
CH3
Propamocarb Carbaryl Carbofuran Aldicarb Pirimicarb
NH
OCH3
CH3
N OH3C NHO
OCH3
O CH3CH3
NHO
OCH3
CH3CH3
NNH ndash CH3O
O
H3C ndash S N(CH3)2
NN
H3C
H3C
N(CH3)2O
O
Diuron Linuron Metoxuron Chlortoluron Pencycuron
Cl
Cl O
NHN(CH3)2 H
N NO
CH3
OCH3Cl
Cl
HN N
O
CH3
CH3Cl
H3CO
HN N
O
CH3
CH3Cl
H3C O
NHN
Cl
Endosulfan Heptachlor Nonachlor Aldrin EndrinClCl
ClCl
ClCl
O = SO
O
ClClCl
Cl
ClClCl
ClCl
Cl
ClCl
ClClCl
ClClCl
ClCl
ClCl
ClClCl
Cl
ClCl
O
Actochlor Metholachlor 44rsquo-DDD 44rsquo-DDE 44rsquo-DDT
NO
O
CH3
CH3C2H5
CH2ClCl
O CH3OCH3
C2H5H3CN
Cl Cl
CHCl2
Cl Cl
CCl2
Cl Cl
CCl3
Difenoconazol Cyproconazol Fenbuconazole Paclobutrazol MetazachlorH3C
NCl Cl
NN
O O
O
OH
CH3
Cl
N NN
NNN
Cl
NC
CH3
CH3
CH3OH
NNN
ClCl
O N NN
H3C CH3
Triazines
NN N
R2 R3
R1 Compound R1 R2 R3
Atrazine Cl NH-C2H5 NH-CH(CH3)2Simazine Cl NH-C2H5 NH-C2H5Terbuthylazine Cl NH-C2H5 NH-C(CH3)3Terbutryn S-CH3 NH-C2H5 NH-C(CH3)3
infomn-netcom wwwmn-netcom4 5
Typical samples preparation Pesticides from natural water
MN AAp MNo 305520
c Column type CHROMABONDreg HR-X 3 mL 60 mg REF 730936
Column conditioning 5 mL methanol then 5 mL ultrapure waterSample aspiration 50 mL water sample is passed through the column by vacuumWashing 5 mL ultrapure water with 5 methanolDrying with gentle nitrogen stream 15 min (storage before elution step at 4 degC is possible for 1 month)Elution 2 x 3 mL methanol then 1 x 3 mL methanol ndash ethyl acetate (7525 vv)Evaporation to dryness after addition of 10 μL internal standardReconstitution in 1 mL of the initial HPLC eluent
Compounds investigated acetochlor alachlor atrazine azoxystrobin carbaryl carbo-furan 3-hydroxycarbofuran chlorfenvinphos chlorpyriphos chlortoluron 1-(34-dichlorophenyl)-3-methyl urea (DCPMU metabolite of diuron) desethylatrazine (DEA metabolite of atrazine) desethylterbuthylazine (DET metabolite of terbuth-ylazine) desisopropylatrazine (DIA metabolite of atrazine) dimethoate dimetomorph diuron hexazinone 1-(4-isopro-pylphenyl)-3-methyl urea (IPPMU metabolite of isoproturon) 1-(4-isopropyl phenyl) urea (IPPU metabolite of isoproturon) isoproturon linuron metazachlor methomyl metolachlor metoxuron pyrimicarb simazine terbuthylazine thiodicarb
We recommend using the CHROMABONDreg vacuum manifold for 12 columns REF 730150
Pesticide residues from fruit and vegetables using the QuEChERS procedureMN AAp MNo 305190
c Column type CHROMABONDreg QuEChERS Mix I REF 730970 CHROMABONDreg QuEChERS Mix III REF 730972
Sample pretreatment The food sample is homogenized in a 50 mL centrifuge tube (REF 730223) with 8 mL acetonitrile and then cleaned up with pre-weighed mixes of salts and adsorbents in a 15 mL centrifuge tubeProcedure for complex food samples The sample is extracted with Mix I then 1 mL supernantant is transferred to the centrifuge tube with Mix III (samples with low fat content) It is shaken centrifuged and analyzed by HPLC Compounds investigated aldicarb sulfone aldicarb desmethyl-pirimicarb desmethyl-formamido-pirimicarb carbofuran paclobutrazol terbutryn pencycuron dioxathion fenpyroximate oxamyl pymetrozin propamocarb dimethoate carbendazim metalaxyl methoxy-fenozide tebufenozide fenoxycarb pyraclostrobin difeno-conazol propargite spinosad methiocarb sulfoxide thio-phanate-m dimethomorph I fludioxonil fenbuconazol famoxadone prosulfocarb dimethomorph II cyproconazol I cyproconazol II hydroxycarbofuran
Transfer into a sample vial eg 15 mL screw neck vials N 9 amber flat bottom label and scale wide opening REF 702284 and ready assembled screw closures N 9 blue center hole with septum silicone PTFE REF 7022871 11
6 x
32
mm
For other applications see wwwmn-netcomaAAs
infomn-netcom wwwmn-netcom4 5
Sample preparation and analysis Fungicides carbendazime and thiabendazole from fruit juiceSolid phase extraction
MN AAp MNo 304740Sample pretreatment see wwwmn-netcomaAAs
c Column type CHROMABONDreg HR-XC 3 mL 200 mg REF 730952
Column conditioning 5 mL methanol then 5 mL 2 NH3 (Do not let run the column dry)Sample aspiration The prepared sample is passed through the column by vacuumWashing impurities are removed with the following washing mixtures a) 2 mL 2 NH3 b) 2 mL 30 methanol ndash 5 NH3 c) 2 mL 01 molL HCl d) 2 mL methanolDrying with nitrogen or airElution 4 mL methanol 5 NH3
It is recommended to filter the prepared sample through dis-posable filters CHROMAFILreg Xtra PET-4525 REF 729220
HPLC analysisMN AAp MNo 122200
Column EC 1254 NUCLEODURreg C18 Gravity 5 μm REF 76010040
0 2 4 6 8 10 min
2
1
Peaks1 Carbendazime
OCH3NH
NHN
O2 ThiabendazoleNN
S NH
Detailed conditions are available online at wwwmn-netcomaAAs
MN suggests using guard columns to protect your main column and significantly increase column life
The Column Protection System (REF 718966) is suitable for all analytical HPLC columns with 116 fittings
infomn-netcom wwwmn-netcom6 7
Analysis Subsequent analysis GCPesticides from cod liver oil
MN AAp MNo 213270R Looser K Ballschmiter J Chromatogr A 836 271ndash284 (1999)Column OPTIMAreg δ-3 60 m x 025 mm ID 025 μm film
REF 72642060
1
4
2
7
8
9
10
11
12
13
1415
16
1719
20
21
2223
24
2527
30
31
33
3432
29
26 2818
3 4
5
6
10 20 30 40 50min
b)
a)
It is recommended to filter the prepared sample through disposable filters CHROMAFILreg PTFE-4525 REF 729205
Organochlorine pesticides (EPA 8081)MN AAp MNo 213630
Column OPTIMAreg 17 MS 30 m x 025 mm ID 025 μm film REF 72616230
Peaks1 2456-Tetrachloro-
m-xylene2 α-BHC3 γ-BHC (Lindane)4 Heptachlor5 β-BHC6 Aldrin7 δ-BHC8 Heptachlor epoxide9 γ-Chlordane10 α-Chlordane11 Endosulfan I
12 44-DDE13 Dieldrin14 Endrin15 44-DDD16 Endosulfan II17 44-DDT18 Endrin aldehyde19 Endosulfan sulfate20 Methoxychlor21 Endrin ketone22 Decachlorobiphenyl
Detailed conditions are available on-line at wwwmn-netcomaAAs
Peaks1 Pentachlorobenzene2 246-Tribromoanisole3 Tetrachloro-14-dimethoxy-
benzene4 α-BHC5 Pentachloroanisole6 HCB7 γ-BHC8 β-BHC9 Heptachlor10 ε-BHC11 Aldrin12 1234-Tetrachloro-
naphthalene (TCN)13 PCB-10314 Oxychlordane15 cis-Heptachlor epoxide
16 24-DDE17 trans-Chlordane18 cis-Chlordane
trans-Nonachlor19 Endosulfan I20 44-DDE21 Dieldrin22 24-DDD23 Endrin24 24-DDT25 cis-Nonachlor26 44-DDD27 Endosulfan II28 44-DDT29 Parlar 4130 Parlar 4031 Parlar 4432 Endosulfan sulphate33 Parlar 5034 Mirex
1
2
3
4
5
6
7 8
9 10
11
12
1314
15
17
18
1920
21
2216
64 12108 14 min
infomn-netcom wwwmn-netcom6 7
Product information Ordering informationStep Product Pack of REFSPE 1 CHROMABONDreg HR-X 3 mL 60 mg 30 730936
CHROMABONDreg vacuum manifold for 12 columns 1 730150SPE 2 (QuEChERS) CHROMABONDreg QuEChERS Mix I (citrate extraction mix) 50 730970
CHROMABONDreg QuEChERS Mix III (Diamino clean-up mix) 50 730972SPE 3 CHROMABONDreg HR-XC 3 mL 200 mg 30 730952
CHROMABONDreg vacuum manifold for 12 columns 1 730150Filtration Disposable filters CHROMAFILreg Xtra PET-4525 100 729220
Disposable filters CHROMAFILreg Xtra PTFE-4525 100 729205Vials and caps 15 mL screw neck vials N 9 amber label and scale 100 702284
Ready assembled screw closures N 9 blue center hole with septum Silicone white PTFE red
100 7022871
HPLCColumn EC 1254 NUCLEODURreg C18 Gravity 5 μm 1 76010040Guard column EC 43 NUCLEODURreg C18 Gravity 5 μm 3 76190330Guard column holder Column Protection System 1 718966GC 1Column OPTIMAreg δ-3 60 m x 025 mm ID 025 μm film 1 72642060GC 2Column OPTIMAreg 17 MS 30 m x 025 mm ID 025 μm film 1 72616230
Visit us at wwwmn-netcomchroma to get more helpful information
Selection tools for
Vials and caps
Syringe filters
Troubleshooting guidesGC and HPLC
Detailed product information
hellipand much more
wwwmn-netcom
wwwmn-netcomMACHEREY-NAGEL
EN ISO 9001 2008CERTIFIED
Food safety made easy with products from
MACHEREY-NAGEL
Vol 8
Additives
Vol 6
Mycotoxins
Vol 2
Vitamins
Vol 4
Acrylamide
Vol 1
Melamine
Vol 5
Sugars Sweeteners
Vol 3
AntibioticsVol 7
Pesticides
KATE
N20
0123
Foc
us F
lyer
Foo
d Vo
l 7
en1
10
031
5 PD
Pr
inte
d in
Ger
man
yIm
age
cred
its copy
Tija
na o
lga
dem
schi
shin
a P
ierre
bril
lot
Pete
r Her
mes
Fur
ian
ar
enaf
otou
k fo
vito
nic
eday
rr1
45 a
irbor
ne77
Sl
y SA
DEQ
ndash fo
tolia
com
infomn-netcom wwwmn-netcom2 3
Focus on pesticides The global production and trading of food and feed products makes monitoring and control difficult
Only analysis of food and raw materials for the production of food can ensure safe and edible food Food safety is an essential part of our quality of life
MN shows examples for food analysis that will help you with your work and make our food safer
Vol 7 Pesticides in foodPesticides are chemical agents used for prevention and control of various pests Pesticides can be classified in herbicides (to control weeds) insecticides (to control in-sects) fungicides (to control molds and fungi) and roden-ticides (to control rodents) [EPA ndash US Environmental Protec-tion Agency ndash httpwwwepagovpesticidesfood]
Most commonly used are
bullOrganophosphates
bullCarbamates
bullPyrethroids
bullSulfonylurea
bullGlyphosate
bullOrganochlorine compounds
Since pesticides can be harmful to health of humans an-imals or the environment they need to be monitored and very responsibly used [EPA ndash US Environmental Protection Agency ndash httpwwwepagovpesticideshealthhumanhtm]
Among other authorities the US Environmental Protec-tion Agency (EPA) sets limits on how much of a pesticide can be used on food during the growing and processing and how much is allowed to remain in the food [EPA ndash US Environmental Protection Agency ndash httpwwwepagovpesti-cidessafetyapplicatorsrestricthtm]
infomn-netcom wwwmn-netcom2 3
Compounds of interestChemical structures of selected pesticides
Dioxathion Dimethoate Glyphosate Chlorpyriphos Chlorfenvinphos
PP
OC2H5H5C2O
OC2H5H5C2O
SS
SS
OO
PH3CO
OCH3
SSN
H
OH3C
OOP
HN OHHO
HO
PH5C2O
OC2H5
SO
N
Cl
Cl
Cl
Cl
Cl
O
OPO O
Cl
CH3
CH3
Propamocarb Carbaryl Carbofuran Aldicarb Pirimicarb
NH
OCH3
CH3
N OH3C NHO
OCH3
O CH3CH3
NHO
OCH3
CH3CH3
NNH ndash CH3O
O
H3C ndash S N(CH3)2
NN
H3C
H3C
N(CH3)2O
O
Diuron Linuron Metoxuron Chlortoluron Pencycuron
Cl
Cl O
NHN(CH3)2 H
N NO
CH3
OCH3Cl
Cl
HN N
O
CH3
CH3Cl
H3CO
HN N
O
CH3
CH3Cl
H3C O
NHN
Cl
Endosulfan Heptachlor Nonachlor Aldrin EndrinClCl
ClCl
ClCl
O = SO
O
ClClCl
Cl
ClClCl
ClCl
Cl
ClCl
ClClCl
ClClCl
ClCl
ClCl
ClClCl
Cl
ClCl
O
Actochlor Metholachlor 44rsquo-DDD 44rsquo-DDE 44rsquo-DDT
NO
O
CH3
CH3C2H5
CH2ClCl
O CH3OCH3
C2H5H3CN
Cl Cl
CHCl2
Cl Cl
CCl2
Cl Cl
CCl3
Difenoconazol Cyproconazol Fenbuconazole Paclobutrazol MetazachlorH3C
NCl Cl
NN
O O
O
OH
CH3
Cl
N NN
NNN
Cl
NC
CH3
CH3
CH3OH
NNN
ClCl
O N NN
H3C CH3
Triazines
NN N
R2 R3
R1 Compound R1 R2 R3
Atrazine Cl NH-C2H5 NH-CH(CH3)2Simazine Cl NH-C2H5 NH-C2H5Terbuthylazine Cl NH-C2H5 NH-C(CH3)3Terbutryn S-CH3 NH-C2H5 NH-C(CH3)3
infomn-netcom wwwmn-netcom4 5
Typical samples preparation Pesticides from natural water
MN AAp MNo 305520
c Column type CHROMABONDreg HR-X 3 mL 60 mg REF 730936
Column conditioning 5 mL methanol then 5 mL ultrapure waterSample aspiration 50 mL water sample is passed through the column by vacuumWashing 5 mL ultrapure water with 5 methanolDrying with gentle nitrogen stream 15 min (storage before elution step at 4 degC is possible for 1 month)Elution 2 x 3 mL methanol then 1 x 3 mL methanol ndash ethyl acetate (7525 vv)Evaporation to dryness after addition of 10 μL internal standardReconstitution in 1 mL of the initial HPLC eluent
Compounds investigated acetochlor alachlor atrazine azoxystrobin carbaryl carbo-furan 3-hydroxycarbofuran chlorfenvinphos chlorpyriphos chlortoluron 1-(34-dichlorophenyl)-3-methyl urea (DCPMU metabolite of diuron) desethylatrazine (DEA metabolite of atrazine) desethylterbuthylazine (DET metabolite of terbuth-ylazine) desisopropylatrazine (DIA metabolite of atrazine) dimethoate dimetomorph diuron hexazinone 1-(4-isopro-pylphenyl)-3-methyl urea (IPPMU metabolite of isoproturon) 1-(4-isopropyl phenyl) urea (IPPU metabolite of isoproturon) isoproturon linuron metazachlor methomyl metolachlor metoxuron pyrimicarb simazine terbuthylazine thiodicarb
We recommend using the CHROMABONDreg vacuum manifold for 12 columns REF 730150
Pesticide residues from fruit and vegetables using the QuEChERS procedureMN AAp MNo 305190
c Column type CHROMABONDreg QuEChERS Mix I REF 730970 CHROMABONDreg QuEChERS Mix III REF 730972
Sample pretreatment The food sample is homogenized in a 50 mL centrifuge tube (REF 730223) with 8 mL acetonitrile and then cleaned up with pre-weighed mixes of salts and adsorbents in a 15 mL centrifuge tubeProcedure for complex food samples The sample is extracted with Mix I then 1 mL supernantant is transferred to the centrifuge tube with Mix III (samples with low fat content) It is shaken centrifuged and analyzed by HPLC Compounds investigated aldicarb sulfone aldicarb desmethyl-pirimicarb desmethyl-formamido-pirimicarb carbofuran paclobutrazol terbutryn pencycuron dioxathion fenpyroximate oxamyl pymetrozin propamocarb dimethoate carbendazim metalaxyl methoxy-fenozide tebufenozide fenoxycarb pyraclostrobin difeno-conazol propargite spinosad methiocarb sulfoxide thio-phanate-m dimethomorph I fludioxonil fenbuconazol famoxadone prosulfocarb dimethomorph II cyproconazol I cyproconazol II hydroxycarbofuran
Transfer into a sample vial eg 15 mL screw neck vials N 9 amber flat bottom label and scale wide opening REF 702284 and ready assembled screw closures N 9 blue center hole with septum silicone PTFE REF 7022871 11
6 x
32
mm
For other applications see wwwmn-netcomaAAs
infomn-netcom wwwmn-netcom4 5
Sample preparation and analysis Fungicides carbendazime and thiabendazole from fruit juiceSolid phase extraction
MN AAp MNo 304740Sample pretreatment see wwwmn-netcomaAAs
c Column type CHROMABONDreg HR-XC 3 mL 200 mg REF 730952
Column conditioning 5 mL methanol then 5 mL 2 NH3 (Do not let run the column dry)Sample aspiration The prepared sample is passed through the column by vacuumWashing impurities are removed with the following washing mixtures a) 2 mL 2 NH3 b) 2 mL 30 methanol ndash 5 NH3 c) 2 mL 01 molL HCl d) 2 mL methanolDrying with nitrogen or airElution 4 mL methanol 5 NH3
It is recommended to filter the prepared sample through dis-posable filters CHROMAFILreg Xtra PET-4525 REF 729220
HPLC analysisMN AAp MNo 122200
Column EC 1254 NUCLEODURreg C18 Gravity 5 μm REF 76010040
0 2 4 6 8 10 min
2
1
Peaks1 Carbendazime
OCH3NH
NHN
O2 ThiabendazoleNN
S NH
Detailed conditions are available online at wwwmn-netcomaAAs
MN suggests using guard columns to protect your main column and significantly increase column life
The Column Protection System (REF 718966) is suitable for all analytical HPLC columns with 116 fittings
infomn-netcom wwwmn-netcom6 7
Analysis Subsequent analysis GCPesticides from cod liver oil
MN AAp MNo 213270R Looser K Ballschmiter J Chromatogr A 836 271ndash284 (1999)Column OPTIMAreg δ-3 60 m x 025 mm ID 025 μm film
REF 72642060
1
4
2
7
8
9
10
11
12
13
1415
16
1719
20
21
2223
24
2527
30
31
33
3432
29
26 2818
3 4
5
6
10 20 30 40 50min
b)
a)
It is recommended to filter the prepared sample through disposable filters CHROMAFILreg PTFE-4525 REF 729205
Organochlorine pesticides (EPA 8081)MN AAp MNo 213630
Column OPTIMAreg 17 MS 30 m x 025 mm ID 025 μm film REF 72616230
Peaks1 2456-Tetrachloro-
m-xylene2 α-BHC3 γ-BHC (Lindane)4 Heptachlor5 β-BHC6 Aldrin7 δ-BHC8 Heptachlor epoxide9 γ-Chlordane10 α-Chlordane11 Endosulfan I
12 44-DDE13 Dieldrin14 Endrin15 44-DDD16 Endosulfan II17 44-DDT18 Endrin aldehyde19 Endosulfan sulfate20 Methoxychlor21 Endrin ketone22 Decachlorobiphenyl
Detailed conditions are available on-line at wwwmn-netcomaAAs
Peaks1 Pentachlorobenzene2 246-Tribromoanisole3 Tetrachloro-14-dimethoxy-
benzene4 α-BHC5 Pentachloroanisole6 HCB7 γ-BHC8 β-BHC9 Heptachlor10 ε-BHC11 Aldrin12 1234-Tetrachloro-
naphthalene (TCN)13 PCB-10314 Oxychlordane15 cis-Heptachlor epoxide
16 24-DDE17 trans-Chlordane18 cis-Chlordane
trans-Nonachlor19 Endosulfan I20 44-DDE21 Dieldrin22 24-DDD23 Endrin24 24-DDT25 cis-Nonachlor26 44-DDD27 Endosulfan II28 44-DDT29 Parlar 4130 Parlar 4031 Parlar 4432 Endosulfan sulphate33 Parlar 5034 Mirex
1
2
3
4
5
6
7 8
9 10
11
12
1314
15
17
18
1920
21
2216
64 12108 14 min
infomn-netcom wwwmn-netcom6 7
Product information Ordering informationStep Product Pack of REFSPE 1 CHROMABONDreg HR-X 3 mL 60 mg 30 730936
CHROMABONDreg vacuum manifold for 12 columns 1 730150SPE 2 (QuEChERS) CHROMABONDreg QuEChERS Mix I (citrate extraction mix) 50 730970
CHROMABONDreg QuEChERS Mix III (Diamino clean-up mix) 50 730972SPE 3 CHROMABONDreg HR-XC 3 mL 200 mg 30 730952
CHROMABONDreg vacuum manifold for 12 columns 1 730150Filtration Disposable filters CHROMAFILreg Xtra PET-4525 100 729220
Disposable filters CHROMAFILreg Xtra PTFE-4525 100 729205Vials and caps 15 mL screw neck vials N 9 amber label and scale 100 702284
Ready assembled screw closures N 9 blue center hole with septum Silicone white PTFE red
100 7022871
HPLCColumn EC 1254 NUCLEODURreg C18 Gravity 5 μm 1 76010040Guard column EC 43 NUCLEODURreg C18 Gravity 5 μm 3 76190330Guard column holder Column Protection System 1 718966GC 1Column OPTIMAreg δ-3 60 m x 025 mm ID 025 μm film 1 72642060GC 2Column OPTIMAreg 17 MS 30 m x 025 mm ID 025 μm film 1 72616230
Visit us at wwwmn-netcomchroma to get more helpful information
Selection tools for
Vials and caps
Syringe filters
Troubleshooting guidesGC and HPLC
Detailed product information
hellipand much more
wwwmn-netcom
wwwmn-netcomMACHEREY-NAGEL
EN ISO 9001 2008CERTIFIED
Food safety made easy with products from
MACHEREY-NAGEL
Vol 8
Additives
Vol 6
Mycotoxins
Vol 2
Vitamins
Vol 4
Acrylamide
Vol 1
Melamine
Vol 5
Sugars Sweeteners
Vol 3
AntibioticsVol 7
Pesticides
KATE
N20
0123
Foc
us F
lyer
Foo
d Vo
l 7
en1
10
031
5 PD
Pr
inte
d in
Ger
man
yIm
age
cred
its copy
Tija
na o
lga
dem
schi
shin
a P
ierre
bril
lot
Pete
r Her
mes
Fur
ian
ar
enaf
otou
k fo
vito
nic
eday
rr1
45 a
irbor
ne77
Sl
y SA
DEQ
ndash fo
tolia
com
infomn-netcom wwwmn-netcom2 3
Compounds of interestChemical structures of selected pesticides
Dioxathion Dimethoate Glyphosate Chlorpyriphos Chlorfenvinphos
PP
OC2H5H5C2O
OC2H5H5C2O
SS
SS
OO
PH3CO
OCH3
SSN
H
OH3C
OOP
HN OHHO
HO
PH5C2O
OC2H5
SO
N
Cl
Cl
Cl
Cl
Cl
O
OPO O
Cl
CH3
CH3
Propamocarb Carbaryl Carbofuran Aldicarb Pirimicarb
NH
OCH3
CH3
N OH3C NHO
OCH3
O CH3CH3
NHO
OCH3
CH3CH3
NNH ndash CH3O
O
H3C ndash S N(CH3)2
NN
H3C
H3C
N(CH3)2O
O
Diuron Linuron Metoxuron Chlortoluron Pencycuron
Cl
Cl O
NHN(CH3)2 H
N NO
CH3
OCH3Cl
Cl
HN N
O
CH3
CH3Cl
H3CO
HN N
O
CH3
CH3Cl
H3C O
NHN
Cl
Endosulfan Heptachlor Nonachlor Aldrin EndrinClCl
ClCl
ClCl
O = SO
O
ClClCl
Cl
ClClCl
ClCl
Cl
ClCl
ClClCl
ClClCl
ClCl
ClCl
ClClCl
Cl
ClCl
O
Actochlor Metholachlor 44rsquo-DDD 44rsquo-DDE 44rsquo-DDT
NO
O
CH3
CH3C2H5
CH2ClCl
O CH3OCH3
C2H5H3CN
Cl Cl
CHCl2
Cl Cl
CCl2
Cl Cl
CCl3
Difenoconazol Cyproconazol Fenbuconazole Paclobutrazol MetazachlorH3C
NCl Cl
NN
O O
O
OH
CH3
Cl
N NN
NNN
Cl
NC
CH3
CH3
CH3OH
NNN
ClCl
O N NN
H3C CH3
Triazines
NN N
R2 R3
R1 Compound R1 R2 R3
Atrazine Cl NH-C2H5 NH-CH(CH3)2Simazine Cl NH-C2H5 NH-C2H5Terbuthylazine Cl NH-C2H5 NH-C(CH3)3Terbutryn S-CH3 NH-C2H5 NH-C(CH3)3
infomn-netcom wwwmn-netcom4 5
Typical samples preparation Pesticides from natural water
MN AAp MNo 305520
c Column type CHROMABONDreg HR-X 3 mL 60 mg REF 730936
Column conditioning 5 mL methanol then 5 mL ultrapure waterSample aspiration 50 mL water sample is passed through the column by vacuumWashing 5 mL ultrapure water with 5 methanolDrying with gentle nitrogen stream 15 min (storage before elution step at 4 degC is possible for 1 month)Elution 2 x 3 mL methanol then 1 x 3 mL methanol ndash ethyl acetate (7525 vv)Evaporation to dryness after addition of 10 μL internal standardReconstitution in 1 mL of the initial HPLC eluent
Compounds investigated acetochlor alachlor atrazine azoxystrobin carbaryl carbo-furan 3-hydroxycarbofuran chlorfenvinphos chlorpyriphos chlortoluron 1-(34-dichlorophenyl)-3-methyl urea (DCPMU metabolite of diuron) desethylatrazine (DEA metabolite of atrazine) desethylterbuthylazine (DET metabolite of terbuth-ylazine) desisopropylatrazine (DIA metabolite of atrazine) dimethoate dimetomorph diuron hexazinone 1-(4-isopro-pylphenyl)-3-methyl urea (IPPMU metabolite of isoproturon) 1-(4-isopropyl phenyl) urea (IPPU metabolite of isoproturon) isoproturon linuron metazachlor methomyl metolachlor metoxuron pyrimicarb simazine terbuthylazine thiodicarb
We recommend using the CHROMABONDreg vacuum manifold for 12 columns REF 730150
Pesticide residues from fruit and vegetables using the QuEChERS procedureMN AAp MNo 305190
c Column type CHROMABONDreg QuEChERS Mix I REF 730970 CHROMABONDreg QuEChERS Mix III REF 730972
Sample pretreatment The food sample is homogenized in a 50 mL centrifuge tube (REF 730223) with 8 mL acetonitrile and then cleaned up with pre-weighed mixes of salts and adsorbents in a 15 mL centrifuge tubeProcedure for complex food samples The sample is extracted with Mix I then 1 mL supernantant is transferred to the centrifuge tube with Mix III (samples with low fat content) It is shaken centrifuged and analyzed by HPLC Compounds investigated aldicarb sulfone aldicarb desmethyl-pirimicarb desmethyl-formamido-pirimicarb carbofuran paclobutrazol terbutryn pencycuron dioxathion fenpyroximate oxamyl pymetrozin propamocarb dimethoate carbendazim metalaxyl methoxy-fenozide tebufenozide fenoxycarb pyraclostrobin difeno-conazol propargite spinosad methiocarb sulfoxide thio-phanate-m dimethomorph I fludioxonil fenbuconazol famoxadone prosulfocarb dimethomorph II cyproconazol I cyproconazol II hydroxycarbofuran
Transfer into a sample vial eg 15 mL screw neck vials N 9 amber flat bottom label and scale wide opening REF 702284 and ready assembled screw closures N 9 blue center hole with septum silicone PTFE REF 7022871 11
6 x
32
mm
For other applications see wwwmn-netcomaAAs
infomn-netcom wwwmn-netcom4 5
Sample preparation and analysis Fungicides carbendazime and thiabendazole from fruit juiceSolid phase extraction
MN AAp MNo 304740Sample pretreatment see wwwmn-netcomaAAs
c Column type CHROMABONDreg HR-XC 3 mL 200 mg REF 730952
Column conditioning 5 mL methanol then 5 mL 2 NH3 (Do not let run the column dry)Sample aspiration The prepared sample is passed through the column by vacuumWashing impurities are removed with the following washing mixtures a) 2 mL 2 NH3 b) 2 mL 30 methanol ndash 5 NH3 c) 2 mL 01 molL HCl d) 2 mL methanolDrying with nitrogen or airElution 4 mL methanol 5 NH3
It is recommended to filter the prepared sample through dis-posable filters CHROMAFILreg Xtra PET-4525 REF 729220
HPLC analysisMN AAp MNo 122200
Column EC 1254 NUCLEODURreg C18 Gravity 5 μm REF 76010040
0 2 4 6 8 10 min
2
1
Peaks1 Carbendazime
OCH3NH
NHN
O2 ThiabendazoleNN
S NH
Detailed conditions are available online at wwwmn-netcomaAAs
MN suggests using guard columns to protect your main column and significantly increase column life
The Column Protection System (REF 718966) is suitable for all analytical HPLC columns with 116 fittings
infomn-netcom wwwmn-netcom6 7
Analysis Subsequent analysis GCPesticides from cod liver oil
MN AAp MNo 213270R Looser K Ballschmiter J Chromatogr A 836 271ndash284 (1999)Column OPTIMAreg δ-3 60 m x 025 mm ID 025 μm film
REF 72642060
1
4
2
7
8
9
10
11
12
13
1415
16
1719
20
21
2223
24
2527
30
31
33
3432
29
26 2818
3 4
5
6
10 20 30 40 50min
b)
a)
It is recommended to filter the prepared sample through disposable filters CHROMAFILreg PTFE-4525 REF 729205
Organochlorine pesticides (EPA 8081)MN AAp MNo 213630
Column OPTIMAreg 17 MS 30 m x 025 mm ID 025 μm film REF 72616230
Peaks1 2456-Tetrachloro-
m-xylene2 α-BHC3 γ-BHC (Lindane)4 Heptachlor5 β-BHC6 Aldrin7 δ-BHC8 Heptachlor epoxide9 γ-Chlordane10 α-Chlordane11 Endosulfan I
12 44-DDE13 Dieldrin14 Endrin15 44-DDD16 Endosulfan II17 44-DDT18 Endrin aldehyde19 Endosulfan sulfate20 Methoxychlor21 Endrin ketone22 Decachlorobiphenyl
Detailed conditions are available on-line at wwwmn-netcomaAAs
Peaks1 Pentachlorobenzene2 246-Tribromoanisole3 Tetrachloro-14-dimethoxy-
benzene4 α-BHC5 Pentachloroanisole6 HCB7 γ-BHC8 β-BHC9 Heptachlor10 ε-BHC11 Aldrin12 1234-Tetrachloro-
naphthalene (TCN)13 PCB-10314 Oxychlordane15 cis-Heptachlor epoxide
16 24-DDE17 trans-Chlordane18 cis-Chlordane
trans-Nonachlor19 Endosulfan I20 44-DDE21 Dieldrin22 24-DDD23 Endrin24 24-DDT25 cis-Nonachlor26 44-DDD27 Endosulfan II28 44-DDT29 Parlar 4130 Parlar 4031 Parlar 4432 Endosulfan sulphate33 Parlar 5034 Mirex
1
2
3
4
5
6
7 8
9 10
11
12
1314
15
17
18
1920
21
2216
64 12108 14 min
infomn-netcom wwwmn-netcom6 7
Product information Ordering informationStep Product Pack of REFSPE 1 CHROMABONDreg HR-X 3 mL 60 mg 30 730936
CHROMABONDreg vacuum manifold for 12 columns 1 730150SPE 2 (QuEChERS) CHROMABONDreg QuEChERS Mix I (citrate extraction mix) 50 730970
CHROMABONDreg QuEChERS Mix III (Diamino clean-up mix) 50 730972SPE 3 CHROMABONDreg HR-XC 3 mL 200 mg 30 730952
CHROMABONDreg vacuum manifold for 12 columns 1 730150Filtration Disposable filters CHROMAFILreg Xtra PET-4525 100 729220
Disposable filters CHROMAFILreg Xtra PTFE-4525 100 729205Vials and caps 15 mL screw neck vials N 9 amber label and scale 100 702284
Ready assembled screw closures N 9 blue center hole with septum Silicone white PTFE red
100 7022871
HPLCColumn EC 1254 NUCLEODURreg C18 Gravity 5 μm 1 76010040Guard column EC 43 NUCLEODURreg C18 Gravity 5 μm 3 76190330Guard column holder Column Protection System 1 718966GC 1Column OPTIMAreg δ-3 60 m x 025 mm ID 025 μm film 1 72642060GC 2Column OPTIMAreg 17 MS 30 m x 025 mm ID 025 μm film 1 72616230
Visit us at wwwmn-netcomchroma to get more helpful information
Selection tools for
Vials and caps
Syringe filters
Troubleshooting guidesGC and HPLC
Detailed product information
hellipand much more
wwwmn-netcom
wwwmn-netcomMACHEREY-NAGEL
EN ISO 9001 2008CERTIFIED
Food safety made easy with products from
MACHEREY-NAGEL
Vol 8
Additives
Vol 6
Mycotoxins
Vol 2
Vitamins
Vol 4
Acrylamide
Vol 1
Melamine
Vol 5
Sugars Sweeteners
Vol 3
AntibioticsVol 7
Pesticides
KATE
N20
0123
Foc
us F
lyer
Foo
d Vo
l 7
en1
10
031
5 PD
Pr
inte
d in
Ger
man
yIm
age
cred
its copy
Tija
na o
lga
dem
schi
shin
a P
ierre
bril
lot
Pete
r Her
mes
Fur
ian
ar
enaf
otou
k fo
vito
nic
eday
rr1
45 a
irbor
ne77
Sl
y SA
DEQ
ndash fo
tolia
com
infomn-netcom wwwmn-netcom4 5
Typical samples preparation Pesticides from natural water
MN AAp MNo 305520
c Column type CHROMABONDreg HR-X 3 mL 60 mg REF 730936
Column conditioning 5 mL methanol then 5 mL ultrapure waterSample aspiration 50 mL water sample is passed through the column by vacuumWashing 5 mL ultrapure water with 5 methanolDrying with gentle nitrogen stream 15 min (storage before elution step at 4 degC is possible for 1 month)Elution 2 x 3 mL methanol then 1 x 3 mL methanol ndash ethyl acetate (7525 vv)Evaporation to dryness after addition of 10 μL internal standardReconstitution in 1 mL of the initial HPLC eluent
Compounds investigated acetochlor alachlor atrazine azoxystrobin carbaryl carbo-furan 3-hydroxycarbofuran chlorfenvinphos chlorpyriphos chlortoluron 1-(34-dichlorophenyl)-3-methyl urea (DCPMU metabolite of diuron) desethylatrazine (DEA metabolite of atrazine) desethylterbuthylazine (DET metabolite of terbuth-ylazine) desisopropylatrazine (DIA metabolite of atrazine) dimethoate dimetomorph diuron hexazinone 1-(4-isopro-pylphenyl)-3-methyl urea (IPPMU metabolite of isoproturon) 1-(4-isopropyl phenyl) urea (IPPU metabolite of isoproturon) isoproturon linuron metazachlor methomyl metolachlor metoxuron pyrimicarb simazine terbuthylazine thiodicarb
We recommend using the CHROMABONDreg vacuum manifold for 12 columns REF 730150
Pesticide residues from fruit and vegetables using the QuEChERS procedureMN AAp MNo 305190
c Column type CHROMABONDreg QuEChERS Mix I REF 730970 CHROMABONDreg QuEChERS Mix III REF 730972
Sample pretreatment The food sample is homogenized in a 50 mL centrifuge tube (REF 730223) with 8 mL acetonitrile and then cleaned up with pre-weighed mixes of salts and adsorbents in a 15 mL centrifuge tubeProcedure for complex food samples The sample is extracted with Mix I then 1 mL supernantant is transferred to the centrifuge tube with Mix III (samples with low fat content) It is shaken centrifuged and analyzed by HPLC Compounds investigated aldicarb sulfone aldicarb desmethyl-pirimicarb desmethyl-formamido-pirimicarb carbofuran paclobutrazol terbutryn pencycuron dioxathion fenpyroximate oxamyl pymetrozin propamocarb dimethoate carbendazim metalaxyl methoxy-fenozide tebufenozide fenoxycarb pyraclostrobin difeno-conazol propargite spinosad methiocarb sulfoxide thio-phanate-m dimethomorph I fludioxonil fenbuconazol famoxadone prosulfocarb dimethomorph II cyproconazol I cyproconazol II hydroxycarbofuran
Transfer into a sample vial eg 15 mL screw neck vials N 9 amber flat bottom label and scale wide opening REF 702284 and ready assembled screw closures N 9 blue center hole with septum silicone PTFE REF 7022871 11
6 x
32
mm
For other applications see wwwmn-netcomaAAs
infomn-netcom wwwmn-netcom4 5
Sample preparation and analysis Fungicides carbendazime and thiabendazole from fruit juiceSolid phase extraction
MN AAp MNo 304740Sample pretreatment see wwwmn-netcomaAAs
c Column type CHROMABONDreg HR-XC 3 mL 200 mg REF 730952
Column conditioning 5 mL methanol then 5 mL 2 NH3 (Do not let run the column dry)Sample aspiration The prepared sample is passed through the column by vacuumWashing impurities are removed with the following washing mixtures a) 2 mL 2 NH3 b) 2 mL 30 methanol ndash 5 NH3 c) 2 mL 01 molL HCl d) 2 mL methanolDrying with nitrogen or airElution 4 mL methanol 5 NH3
It is recommended to filter the prepared sample through dis-posable filters CHROMAFILreg Xtra PET-4525 REF 729220
HPLC analysisMN AAp MNo 122200
Column EC 1254 NUCLEODURreg C18 Gravity 5 μm REF 76010040
0 2 4 6 8 10 min
2
1
Peaks1 Carbendazime
OCH3NH
NHN
O2 ThiabendazoleNN
S NH
Detailed conditions are available online at wwwmn-netcomaAAs
MN suggests using guard columns to protect your main column and significantly increase column life
The Column Protection System (REF 718966) is suitable for all analytical HPLC columns with 116 fittings
infomn-netcom wwwmn-netcom6 7
Analysis Subsequent analysis GCPesticides from cod liver oil
MN AAp MNo 213270R Looser K Ballschmiter J Chromatogr A 836 271ndash284 (1999)Column OPTIMAreg δ-3 60 m x 025 mm ID 025 μm film
REF 72642060
1
4
2
7
8
9
10
11
12
13
1415
16
1719
20
21
2223
24
2527
30
31
33
3432
29
26 2818
3 4
5
6
10 20 30 40 50min
b)
a)
It is recommended to filter the prepared sample through disposable filters CHROMAFILreg PTFE-4525 REF 729205
Organochlorine pesticides (EPA 8081)MN AAp MNo 213630
Column OPTIMAreg 17 MS 30 m x 025 mm ID 025 μm film REF 72616230
Peaks1 2456-Tetrachloro-
m-xylene2 α-BHC3 γ-BHC (Lindane)4 Heptachlor5 β-BHC6 Aldrin7 δ-BHC8 Heptachlor epoxide9 γ-Chlordane10 α-Chlordane11 Endosulfan I
12 44-DDE13 Dieldrin14 Endrin15 44-DDD16 Endosulfan II17 44-DDT18 Endrin aldehyde19 Endosulfan sulfate20 Methoxychlor21 Endrin ketone22 Decachlorobiphenyl
Detailed conditions are available on-line at wwwmn-netcomaAAs
Peaks1 Pentachlorobenzene2 246-Tribromoanisole3 Tetrachloro-14-dimethoxy-
benzene4 α-BHC5 Pentachloroanisole6 HCB7 γ-BHC8 β-BHC9 Heptachlor10 ε-BHC11 Aldrin12 1234-Tetrachloro-
naphthalene (TCN)13 PCB-10314 Oxychlordane15 cis-Heptachlor epoxide
16 24-DDE17 trans-Chlordane18 cis-Chlordane
trans-Nonachlor19 Endosulfan I20 44-DDE21 Dieldrin22 24-DDD23 Endrin24 24-DDT25 cis-Nonachlor26 44-DDD27 Endosulfan II28 44-DDT29 Parlar 4130 Parlar 4031 Parlar 4432 Endosulfan sulphate33 Parlar 5034 Mirex
1
2
3
4
5
6
7 8
9 10
11
12
1314
15
17
18
1920
21
2216
64 12108 14 min
infomn-netcom wwwmn-netcom6 7
Product information Ordering informationStep Product Pack of REFSPE 1 CHROMABONDreg HR-X 3 mL 60 mg 30 730936
CHROMABONDreg vacuum manifold for 12 columns 1 730150SPE 2 (QuEChERS) CHROMABONDreg QuEChERS Mix I (citrate extraction mix) 50 730970
CHROMABONDreg QuEChERS Mix III (Diamino clean-up mix) 50 730972SPE 3 CHROMABONDreg HR-XC 3 mL 200 mg 30 730952
CHROMABONDreg vacuum manifold for 12 columns 1 730150Filtration Disposable filters CHROMAFILreg Xtra PET-4525 100 729220
Disposable filters CHROMAFILreg Xtra PTFE-4525 100 729205Vials and caps 15 mL screw neck vials N 9 amber label and scale 100 702284
Ready assembled screw closures N 9 blue center hole with septum Silicone white PTFE red
100 7022871
HPLCColumn EC 1254 NUCLEODURreg C18 Gravity 5 μm 1 76010040Guard column EC 43 NUCLEODURreg C18 Gravity 5 μm 3 76190330Guard column holder Column Protection System 1 718966GC 1Column OPTIMAreg δ-3 60 m x 025 mm ID 025 μm film 1 72642060GC 2Column OPTIMAreg 17 MS 30 m x 025 mm ID 025 μm film 1 72616230
Visit us at wwwmn-netcomchroma to get more helpful information
Selection tools for
Vials and caps
Syringe filters
Troubleshooting guidesGC and HPLC
Detailed product information
hellipand much more
wwwmn-netcom
wwwmn-netcomMACHEREY-NAGEL
EN ISO 9001 2008CERTIFIED
Food safety made easy with products from
MACHEREY-NAGEL
Vol 8
Additives
Vol 6
Mycotoxins
Vol 2
Vitamins
Vol 4
Acrylamide
Vol 1
Melamine
Vol 5
Sugars Sweeteners
Vol 3
AntibioticsVol 7
Pesticides
KATE
N20
0123
Foc
us F
lyer
Foo
d Vo
l 7
en1
10
031
5 PD
Pr
inte
d in
Ger
man
yIm
age
cred
its copy
Tija
na o
lga
dem
schi
shin
a P
ierre
bril
lot
Pete
r Her
mes
Fur
ian
ar
enaf
otou
k fo
vito
nic
eday
rr1
45 a
irbor
ne77
Sl
y SA
DEQ
ndash fo
tolia
com
infomn-netcom wwwmn-netcom4 5
Sample preparation and analysis Fungicides carbendazime and thiabendazole from fruit juiceSolid phase extraction
MN AAp MNo 304740Sample pretreatment see wwwmn-netcomaAAs
c Column type CHROMABONDreg HR-XC 3 mL 200 mg REF 730952
Column conditioning 5 mL methanol then 5 mL 2 NH3 (Do not let run the column dry)Sample aspiration The prepared sample is passed through the column by vacuumWashing impurities are removed with the following washing mixtures a) 2 mL 2 NH3 b) 2 mL 30 methanol ndash 5 NH3 c) 2 mL 01 molL HCl d) 2 mL methanolDrying with nitrogen or airElution 4 mL methanol 5 NH3
It is recommended to filter the prepared sample through dis-posable filters CHROMAFILreg Xtra PET-4525 REF 729220
HPLC analysisMN AAp MNo 122200
Column EC 1254 NUCLEODURreg C18 Gravity 5 μm REF 76010040
0 2 4 6 8 10 min
2
1
Peaks1 Carbendazime
OCH3NH
NHN
O2 ThiabendazoleNN
S NH
Detailed conditions are available online at wwwmn-netcomaAAs
MN suggests using guard columns to protect your main column and significantly increase column life
The Column Protection System (REF 718966) is suitable for all analytical HPLC columns with 116 fittings
infomn-netcom wwwmn-netcom6 7
Analysis Subsequent analysis GCPesticides from cod liver oil
MN AAp MNo 213270R Looser K Ballschmiter J Chromatogr A 836 271ndash284 (1999)Column OPTIMAreg δ-3 60 m x 025 mm ID 025 μm film
REF 72642060
1
4
2
7
8
9
10
11
12
13
1415
16
1719
20
21
2223
24
2527
30
31
33
3432
29
26 2818
3 4
5
6
10 20 30 40 50min
b)
a)
It is recommended to filter the prepared sample through disposable filters CHROMAFILreg PTFE-4525 REF 729205
Organochlorine pesticides (EPA 8081)MN AAp MNo 213630
Column OPTIMAreg 17 MS 30 m x 025 mm ID 025 μm film REF 72616230
Peaks1 2456-Tetrachloro-
m-xylene2 α-BHC3 γ-BHC (Lindane)4 Heptachlor5 β-BHC6 Aldrin7 δ-BHC8 Heptachlor epoxide9 γ-Chlordane10 α-Chlordane11 Endosulfan I
12 44-DDE13 Dieldrin14 Endrin15 44-DDD16 Endosulfan II17 44-DDT18 Endrin aldehyde19 Endosulfan sulfate20 Methoxychlor21 Endrin ketone22 Decachlorobiphenyl
Detailed conditions are available on-line at wwwmn-netcomaAAs
Peaks1 Pentachlorobenzene2 246-Tribromoanisole3 Tetrachloro-14-dimethoxy-
benzene4 α-BHC5 Pentachloroanisole6 HCB7 γ-BHC8 β-BHC9 Heptachlor10 ε-BHC11 Aldrin12 1234-Tetrachloro-
naphthalene (TCN)13 PCB-10314 Oxychlordane15 cis-Heptachlor epoxide
16 24-DDE17 trans-Chlordane18 cis-Chlordane
trans-Nonachlor19 Endosulfan I20 44-DDE21 Dieldrin22 24-DDD23 Endrin24 24-DDT25 cis-Nonachlor26 44-DDD27 Endosulfan II28 44-DDT29 Parlar 4130 Parlar 4031 Parlar 4432 Endosulfan sulphate33 Parlar 5034 Mirex
1
2
3
4
5
6
7 8
9 10
11
12
1314
15
17
18
1920
21
2216
64 12108 14 min
infomn-netcom wwwmn-netcom6 7
Product information Ordering informationStep Product Pack of REFSPE 1 CHROMABONDreg HR-X 3 mL 60 mg 30 730936
CHROMABONDreg vacuum manifold for 12 columns 1 730150SPE 2 (QuEChERS) CHROMABONDreg QuEChERS Mix I (citrate extraction mix) 50 730970
CHROMABONDreg QuEChERS Mix III (Diamino clean-up mix) 50 730972SPE 3 CHROMABONDreg HR-XC 3 mL 200 mg 30 730952
CHROMABONDreg vacuum manifold for 12 columns 1 730150Filtration Disposable filters CHROMAFILreg Xtra PET-4525 100 729220
Disposable filters CHROMAFILreg Xtra PTFE-4525 100 729205Vials and caps 15 mL screw neck vials N 9 amber label and scale 100 702284
Ready assembled screw closures N 9 blue center hole with septum Silicone white PTFE red
100 7022871
HPLCColumn EC 1254 NUCLEODURreg C18 Gravity 5 μm 1 76010040Guard column EC 43 NUCLEODURreg C18 Gravity 5 μm 3 76190330Guard column holder Column Protection System 1 718966GC 1Column OPTIMAreg δ-3 60 m x 025 mm ID 025 μm film 1 72642060GC 2Column OPTIMAreg 17 MS 30 m x 025 mm ID 025 μm film 1 72616230
Visit us at wwwmn-netcomchroma to get more helpful information
Selection tools for
Vials and caps
Syringe filters
Troubleshooting guidesGC and HPLC
Detailed product information
hellipand much more
wwwmn-netcom
wwwmn-netcomMACHEREY-NAGEL
EN ISO 9001 2008CERTIFIED
Food safety made easy with products from
MACHEREY-NAGEL
Vol 8
Additives
Vol 6
Mycotoxins
Vol 2
Vitamins
Vol 4
Acrylamide
Vol 1
Melamine
Vol 5
Sugars Sweeteners
Vol 3
AntibioticsVol 7
Pesticides
KATE
N20
0123
Foc
us F
lyer
Foo
d Vo
l 7
en1
10
031
5 PD
Pr
inte
d in
Ger
man
yIm
age
cred
its copy
Tija
na o
lga
dem
schi
shin
a P
ierre
bril
lot
Pete
r Her
mes
Fur
ian
ar
enaf
otou
k fo
vito
nic
eday
rr1
45 a
irbor
ne77
Sl
y SA
DEQ
ndash fo
tolia
com
infomn-netcom wwwmn-netcom6 7
Analysis Subsequent analysis GCPesticides from cod liver oil
MN AAp MNo 213270R Looser K Ballschmiter J Chromatogr A 836 271ndash284 (1999)Column OPTIMAreg δ-3 60 m x 025 mm ID 025 μm film
REF 72642060
1
4
2
7
8
9
10
11
12
13
1415
16
1719
20
21
2223
24
2527
30
31
33
3432
29
26 2818
3 4
5
6
10 20 30 40 50min
b)
a)
It is recommended to filter the prepared sample through disposable filters CHROMAFILreg PTFE-4525 REF 729205
Organochlorine pesticides (EPA 8081)MN AAp MNo 213630
Column OPTIMAreg 17 MS 30 m x 025 mm ID 025 μm film REF 72616230
Peaks1 2456-Tetrachloro-
m-xylene2 α-BHC3 γ-BHC (Lindane)4 Heptachlor5 β-BHC6 Aldrin7 δ-BHC8 Heptachlor epoxide9 γ-Chlordane10 α-Chlordane11 Endosulfan I
12 44-DDE13 Dieldrin14 Endrin15 44-DDD16 Endosulfan II17 44-DDT18 Endrin aldehyde19 Endosulfan sulfate20 Methoxychlor21 Endrin ketone22 Decachlorobiphenyl
Detailed conditions are available on-line at wwwmn-netcomaAAs
Peaks1 Pentachlorobenzene2 246-Tribromoanisole3 Tetrachloro-14-dimethoxy-
benzene4 α-BHC5 Pentachloroanisole6 HCB7 γ-BHC8 β-BHC9 Heptachlor10 ε-BHC11 Aldrin12 1234-Tetrachloro-
naphthalene (TCN)13 PCB-10314 Oxychlordane15 cis-Heptachlor epoxide
16 24-DDE17 trans-Chlordane18 cis-Chlordane
trans-Nonachlor19 Endosulfan I20 44-DDE21 Dieldrin22 24-DDD23 Endrin24 24-DDT25 cis-Nonachlor26 44-DDD27 Endosulfan II28 44-DDT29 Parlar 4130 Parlar 4031 Parlar 4432 Endosulfan sulphate33 Parlar 5034 Mirex
1
2
3
4
5
6
7 8
9 10
11
12
1314
15
17
18
1920
21
2216
64 12108 14 min
infomn-netcom wwwmn-netcom6 7
Product information Ordering informationStep Product Pack of REFSPE 1 CHROMABONDreg HR-X 3 mL 60 mg 30 730936
CHROMABONDreg vacuum manifold for 12 columns 1 730150SPE 2 (QuEChERS) CHROMABONDreg QuEChERS Mix I (citrate extraction mix) 50 730970
CHROMABONDreg QuEChERS Mix III (Diamino clean-up mix) 50 730972SPE 3 CHROMABONDreg HR-XC 3 mL 200 mg 30 730952
CHROMABONDreg vacuum manifold for 12 columns 1 730150Filtration Disposable filters CHROMAFILreg Xtra PET-4525 100 729220
Disposable filters CHROMAFILreg Xtra PTFE-4525 100 729205Vials and caps 15 mL screw neck vials N 9 amber label and scale 100 702284
Ready assembled screw closures N 9 blue center hole with septum Silicone white PTFE red
100 7022871
HPLCColumn EC 1254 NUCLEODURreg C18 Gravity 5 μm 1 76010040Guard column EC 43 NUCLEODURreg C18 Gravity 5 μm 3 76190330Guard column holder Column Protection System 1 718966GC 1Column OPTIMAreg δ-3 60 m x 025 mm ID 025 μm film 1 72642060GC 2Column OPTIMAreg 17 MS 30 m x 025 mm ID 025 μm film 1 72616230
Visit us at wwwmn-netcomchroma to get more helpful information
Selection tools for
Vials and caps
Syringe filters
Troubleshooting guidesGC and HPLC
Detailed product information
hellipand much more
wwwmn-netcom
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Product information Ordering informationStep Product Pack of REFSPE 1 CHROMABONDreg HR-X 3 mL 60 mg 30 730936
CHROMABONDreg vacuum manifold for 12 columns 1 730150SPE 2 (QuEChERS) CHROMABONDreg QuEChERS Mix I (citrate extraction mix) 50 730970
CHROMABONDreg QuEChERS Mix III (Diamino clean-up mix) 50 730972SPE 3 CHROMABONDreg HR-XC 3 mL 200 mg 30 730952
CHROMABONDreg vacuum manifold for 12 columns 1 730150Filtration Disposable filters CHROMAFILreg Xtra PET-4525 100 729220
Disposable filters CHROMAFILreg Xtra PTFE-4525 100 729205Vials and caps 15 mL screw neck vials N 9 amber label and scale 100 702284
Ready assembled screw closures N 9 blue center hole with septum Silicone white PTFE red
100 7022871
HPLCColumn EC 1254 NUCLEODURreg C18 Gravity 5 μm 1 76010040Guard column EC 43 NUCLEODURreg C18 Gravity 5 μm 3 76190330Guard column holder Column Protection System 1 718966GC 1Column OPTIMAreg δ-3 60 m x 025 mm ID 025 μm film 1 72642060GC 2Column OPTIMAreg 17 MS 30 m x 025 mm ID 025 μm film 1 72616230
Visit us at wwwmn-netcomchroma to get more helpful information
Selection tools for
Vials and caps
Syringe filters
Troubleshooting guidesGC and HPLC
Detailed product information
hellipand much more
wwwmn-netcom
wwwmn-netcomMACHEREY-NAGEL
EN ISO 9001 2008CERTIFIED
Food safety made easy with products from
MACHEREY-NAGEL
Vol 8
Additives
Vol 6
Mycotoxins
Vol 2
Vitamins
Vol 4
Acrylamide
Vol 1
Melamine
Vol 5
Sugars Sweeteners
Vol 3
AntibioticsVol 7
Pesticides
KATE
N20
0123
Foc
us F
lyer
Foo
d Vo
l 7
en1
10
031
5 PD
Pr
inte
d in
Ger
man
yIm
age
cred
its copy
Tija
na o
lga
dem
schi
shin
a P
ierre
bril
lot
Pete
r Her
mes
Fur
ian
ar
enaf
otou
k fo
vito
nic
eday
rr1
45 a
irbor
ne77
Sl
y SA
DEQ
ndash fo
tolia
com
wwwmn-netcom
wwwmn-netcomMACHEREY-NAGEL
EN ISO 9001 2008CERTIFIED
Food safety made easy with products from
MACHEREY-NAGEL
Vol 8
Additives
Vol 6
Mycotoxins
Vol 2
Vitamins
Vol 4
Acrylamide
Vol 1
Melamine
Vol 5
Sugars Sweeteners
Vol 3
AntibioticsVol 7
Pesticides
KATE
N20
0123
Foc
us F
lyer
Foo
d Vo
l 7
en1
10
031
5 PD
Pr
inte
d in
Ger
man
yIm
age
cred
its copy
Tija
na o
lga
dem
schi
shin
a P
ierre
bril
lot
Pete
r Her
mes
Fur
ian
ar
enaf
otou
k fo
vito
nic
eday
rr1
45 a
irbor
ne77
Sl
y SA
DEQ
ndash fo
tolia
com