Post on 01-Mar-2018
7/25/2019 CHECKLIST PAPER 3 BIOLOGY.doc
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CHECKLIST BIOLOGY PRACTICAL (BIOLOGY A+ DI HATIKU)
CHP NO. AIM OF THE EXP+
HYPOTHESIS +
MATERIALS&APPARATUS+
DIAGRAM
MAGIC BOX PROCEDURE:
K1 : Preparation of material ! apparat"(#K1)
K$ : Operatin% fi&e' ariale
K* : Operatin% manip"late' ariale
K#: Operatin% repon'in% ariale
K+ : Pre,a"tion (K+)4K1, K2, K3, K4, & K! 3 MARKS
RESULT:* *-1
"2##$%
2'
STUDYING THE MO(EMENT
OF SUBSTANCES ACROSS
THE PLASMA MEMBRANE
AIM:
To t"'. t/e fa,tor infl"en,e t/e
'iff"ion of "tan,e t/ro"%/ a
emi0 permeale memrane-HYPOTHESIS:
T/e 'iff"ion of mole,"le t/ro"%/
emi0permeale memrane i ae'
on t/e ie of mole,"le-MATERIALS&APPARATUS:
Bene'i,t2 ol"tion3 14 Star,/
"penion3 Io'ine ol"tion3 *54
%l",oe ol"tion3 i6in% t"in% an'
,otton trea'3 tet t"e3 ea6er3 an'
B"nen "rnerDIAGRAM:
78 R8 C8
CO9CRETE T.pe o f
ol"tion
(%l",oe
an' tar,/
"penion)
C/an%e in
Color of
ol"tion re"lt
of Bene'i,t2
tet
8ol"me of
%l",oe ol"tion
(1+ml)
8ol"me of
tar,/
"penion (1+
ml)
ABSTRACT Sie of t/e
mole,"le
in t/e
i6in%
t"e
;iff"ion of
"tan,e
t/ro"%/ a emi0
permeale
memrane
7ETHO;S
Oo
t.pe of
ol"tion
(%l",oean' tar,/
"penion)
)R("TECHNI
UE'
Observe and
recordt/e,/an%e in ,olor
of ol"tion
ini'e t/e
i6in% t"e an'
t/e ea6er re"lt
of Bene'i,t2
tet
Fixed t/e
8ol"me of
%l",oe ol"tion
to 1+ml8ol"me of
tar,/
"penion 1+
ml) . using
measuring
cylinder
*R("TECHNI
UE'0
1- Soa6 t/e i6in% t"in% in >ater for + min to
often it- (K1)
$- Tie one en' of i6in% t"e to preent
lea6a%e-(K+)
*- it/ 'itille' >ater- (K1)(K+)
?- 7i&e' io'ine an' 'itille' >ater- Oere'
t/e ,olor(K1)(K#)
@- Immere' t/e i6in% t"e into ea6er for #5
min"te-(K1)
- After #5 min"te3 oere an' re,or' t/e
,/an%e in ,olor of ol"tion ini'e t/e
i6in% t"e an' t/e ea6er(K#)(K*)
- Carr. o"t t/e Bene'i,t2 tet for ot/ ol"tionini'e t/e i6in% t"e an' t/e ea6er(K#)
R-/:Con
tent
Ori%i
nal
Color
ater3 ,otton t/rea'3 retort tan'3
,apillar. t"e3 r"ler3 mar6er pen3,ior3 $+5ml ea6er3 .rin%e3
top>at,/ an' i6in% t"in%-
DIAGRAM:
78 R8 C8CO9CRETE T.pe o f
"tan,e
(>ater an'
",roe)
In,reae t/e
leel of ",roe
ol"tion in
,apillar.
eer.15 min"te
for #+ min"te
,on,entration of
",roe ol"tion
Time ta6en for
mar6in% t/e
leel of ",roe
ol"tion in
,apillar. t"e at
15 min"teinteral for #+
min"te
ABSTRACT Sie of t/e
mole,"le
Rate of
;iff"ion of"tan,e
t/ro"%/ a emi0
permeale
memrane
7ETHO;S
Oo
'ifferent
ol"tion
>/i,/ are
'itille'
>ater
(ini'e t/e
ea6er)an'
*54
",roe
ol"tion in
t/e 8i6in%
t"e
-
)R("TECHNI
UE'Measure and
recordt/e
in,reae t/e
leel of ",roe
ol"tion in
,apillar.
eer.15 min"te
for #+ min"te
by using ruler.
it/ 'itille' >ater- (K1)(K+)
?- Clamp t/e ,apillar. t"e erti,all. to retort
tan'- (K1)
@- Immere' t/e i6in% t"e into ea6er
,ontainin% t/e 'itille' >ater-(K1)
- 7ar6 initial leel of ",roe ol"tion (K1)
- 7ar6 t/e leel of ",roe ol"tion eer.15
min"te for #+ min"te (K*)
15-Measure and recordt/e in,reae t/e leel of
",roe ol"tion in ,apillar. eer.15 min"te
for #+ min"te . "in% r"ler-(K#)
R-/:
Time
(min"te)
T/e In,reae t/e leel of ",roe
ol"tion in ,apillar. (mmmin01)
5
15
$5*5
#5
Score 50/50
7/25/2019 CHECKLIST PAPER 3 BIOLOGY.doc
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(mm)time(min)
=nit0(mmmin01)
*-*"2##0
%1'
STUDYING THE EFFECT OF
HYPOTONIC. HYPERTONIC
ANS ISOTONIC SOLUTION
ON ANIMAL CELL
AIM:To t"'. t/e effe,t of 'ifferent
,on,entration of o'i"m ,/lori'e
(9aCl) ol"tion on re' loo' ,ell >it/in
*5 min"te-
HYPOTHESIS:T/e /i%/er t/e ,on,entration of o'i"m,/lori'e ol"tion t/e /i%/er t/e n"mer
of ,renate' re' loo' ,ell
-MATERIALS&APPARATUS:/i,/ are
5-1+ 73
5-*5 73
5-#+ 7 an'
5-?5 7
)R("TECHNIUE'Count and
record t/e
n"mer of
,renate' re'
loo' ,ell-
Used the same'"ration of time
>/i,/ i 15
min"te for
immerion of
t/e re' loo'
,ell . "in%
t/estopwatch.
*R("TECHNI
UE'0
1- Lael # li'e >it/ A3 B3 C3 an' ;- (K1)
$- P"t a 'rop of 5-1+ 7 o'i"m ,/lori'e on
li'e A- (K1) (K$)
*- Coer t/e li'e >it/ ,oerlip- (K1)
#- P"t one 'rop of loo' (RBC) on t/e ot/er
i'e of li'e A- (K$)
+- Pla,e t/e filter paper on t/e oppoite i'e-
(K1)(K+)
?- After 15 min"te3 ,o"nt an' re,or' t/e
n"mer of ,renate' re' loo' ,ell- (K#)
@- Repeat t/e tep 10@ >it/ 'ifferent,on,entration of o'i"m ,/lori'e ol"tion
>/i,/ are 5-*5 73 5-#+ 7 an' 5-?5 7-(K*)
R-/:C)/*/ 5
6-7 )86
-/ "M'
N-79 5 )*/6
6 96 )
"A'#.1
"B'#.3#
"C'#.4
"D'#.#
*-# STUDYING THE EFFECT OFHYPOTONIC. HYPERTONIC
ANS ISOTONIC SOLUTION
ON PLANT CELLS
AIM:To 'etermine t/e effe,t of 'ifferent
,on,entration of a ol"tion on t/e ma
/ape of potato-
HYPOTHESIS:T/e 8;8 ,on,entration of t/e ",roe
ol"tion3 t/e /i,/ are
5-$43
+-5431+-5
43 an'
$+-54
)R("TECHNI
UE'Measure and
recordt/e
,/an%e in maof potato ti"e
in 'ifferent
,on,entration of
",roe ol"tion
. "in%
alan,e
weighing scale
Fixed the same
'"ration of
oa6in% time
>/i,/ i ?5
min"te forimmerion of
t/e potato .
"in% t/e
stopwatch./
Use the same
ol"me of
",roe ol"tion
>/i,/ i $5 ml
by using
measuring
cylinder.
*R("TECHNI
UE'
0
1- Bore,"t o"t a potato to %et potato trip of
t/e ame ie an' >ei%/ t/em o a to %et
potato trip of t/e ame ma(K1)
$- it/ $5 ml of ",roe
ol"tion of 5-$43 +-543 1+-543 an' $+-54
(K1) (K*)(K$)
*- P"t* of potato trip of t/e ame >ei%/t in
ea,/ ea6er (K1)
#- Leae t/e potato trip immere' in t/e
",roe ol"tion for 1 /o"r(K$)
+- (After 1 /o"r) ta6e o"t t/e potato trip from
ea,/ ea6er3 'r. t/em "in% filter paper(K1)(K+)
?- 7ea"re an' re,or' t/e ,/an%e in ma of
potato ti"e in 'ifferent ,on,entration of
",roe ol"tion . "in% alan,e >ei%/in%
,ale(K1)(K#)
@- Repeat tep 10? t/ree time to %et a,,"rate
'ata (K+)
- Plot a %rap/ of t/e ,on,entration of ",roe
ol"tion a%aint ,/an%e in ma of potato
trip
- Ta"late t/e 'ata in t/e tale elo> (K1)
R-/:C)
/*/
5
-)
-/
"='
C8*; 7* 5 >/*/
/> %;
#.2 1t
rea'
in%
$n'
rea'i
n%
*r'
rea'i
n%
Ae
ra%e
.#
1.#
2.#
;ifferent ,on,entration of
",roe ol"tion
7/25/2019 CHECKLIST PAPER 3 BIOLOGY.doc
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*-+"2##
%2'
"2##
%2'
DETERMINING THE
CONCENTRATION OF
EXTERNAL SOLUTION
?HICH IS ISOTONIC TO THE
CELL SAP OF A PLANT
CELLS
AIM:to 'etermine t/e ,on,entration of t/e
ol"tion >/i,/ >ill maintain t/e ma of
plant ti"e-
HYPOTHESIS:T/e #.4 M",roe ol"tion >ill
maintain t/e ma of t/e plant ti"e-
T/e ,on,entration of ",roe ol"tion
t/at remain len%t/ma of plant ti"e
i iotoni,-
MATERIALS&APPARATUS:
Plant ti"e (Ram"tan)38ario"
,on,entration of ",roe
ol"tion3e&le: 5-173 5-$7 3 5-#73
1-57 an' 1-+7at,/3
Balan,e>ei%/in% ma,/ine-
DIAGRAM:
78 R8 C8CO9CRETE Con,entrati
on of
",roe
ol"tion-
C/an%e in ma T.pe of plant
ti"e3 ol"me
of ol"tion3
oa6in% time3
ie of potato-
ABSTRACT 0 0
)R("TECHNI
UE'
Measure and
recordt/e,/an%e in ma
of plant ti"e
in 'ifferent
,on,entration of
",roe ol"tion
. "in%
alan,e
weighing
machine.
",roe ol"tion
>/i,/ i $5 ml
by using
measuring
cylinder.
*R("TECHNI
UE'
0
1- ei%/ t/e ram"tan ti"e an' re,or' t/e
initial ma(K1)(K#)
$- it/ 155ml ",roe
ol"tion of 5-173 5-$7 3 5-#73 1-57 an'
1-+7(K1) (K*)(K$)
*- P"t * of plant ti"e in ea,/ ea6er (K1)
#- Leae t/e plant ti"e immere' in t/e ",roeol"tion for 1 /o"r(K$)
+- (After 1 /o"r) ta6e o"t t/e plant ti"e from
ea,/ ea6er3 'r. t/em "in% filter paper(K1)
(K+)
?- 7ea"re an' re,or' t/e ,/an%e in ma of
potato ti"e in 'ifferent ,on,entration of",roe ol"tion . "in% alan,e >ei%/in%
ma,/ine-(K1)(K#)
@- Plot a %rap/ of t/e ,on,entration of ",roe
ol"tion a%aint ,/an%e in ma of plant ti"e
(K#)
R-/:C)
/*/
5
-)
-/
"M'
C8*; 7* 5 >/*/
/> %;
1t
rea'
in%
$n'
rea'i
n%
*r'
rea'i
n%
Ae
ra%e
#.1
#.2
#.4
1.#
1.
# #-*"2##0%
2'
STUDYING THE EFFECT OF
TEMPERATURE ON
SALI(ARY AMYLASE
ACTI(ITY
AIM:To ineti%ate t/e effe,t of temperat"re
on aliar. am.lae a,tiit.-
HYPOTHESIS:A t/e temperaturein,reae3 t/e rate of
reaction catalysed by amylase in,reae"ntil optim"m temperat"re
MATERIALS&APPARATUS:Saliar. Am.lae ol"tion3 14 Star,/
S"penion3
Io'ine ol"tion3 ;itille' >ater3
Tet t"e ea6erD Stop>at,/3 'ropper3
>/ite tile >it/ %rooe3 Gla ro'
DIAGRAM:
78 R8 C8CO9CRETE Temperat"r
e
-
T/e time ta6en
for t/e l"e
la,6 ,olo"r of
io'ine to
'iappear (min)
8ol"me of
tar,/
"penion
(+ml)
8ol"me of
ABSTRACT 0 Rate of rea,tion
,atal.e' .
aliar.
am.lae-
7ETHO;S
O
*/-
"C'
T8 /7 /*@ 5 /8
9- 9*)@ )- 5
6 / 6*>>*
"M'
R*/ 5 7 *)//
"71'
15C
$5C
*5C
#5C
1- Laelle' tet t"e a A13 A$3 B13B$3 C13 C$
3;1an' ;$ (K1)
$- + ml of 14 tar,/ "penion are po"re' into
t/e tet t"e A13 B13 C1 an' ;1 "in%
.rin%e-(K1)(K$)
*- $ ml of 5-14 am.lae i a''e' to tet t"e
A$3 B$3 C$ an' ;$(K1)(K$)
#- T/e tet t"e are immere' in >ater at/ at
'ifferent temperat"re for + min"te-(K*)
A1 an' A$ : 15C 3
B1 an' B$ : $5C3C1 an' C$ : *5C3
;1 an' ;$ : #5C+- ;rop of io'ine ol"tion are a''e' eparatel.
onto t/e %rooe of a >/ite tile "in% a
.rin%e-(K1)
?- After + min"te3 po"r tar,/ "penion (A1)
to am.lae ol"tion (A$) (K1)
@- Stirre' t/e mi&t"re . "in% t/e %la ro' (K1
- Stop>at,/ i a,tiate' imme'iatel. at 5
min"te-(K+)
- A 'rop of mi&t"re i tete' >it/ io'ine
ol"tion on t/e >/ite tile- At eer. amplin%
t/e 'ropper m"t e rine' >it/ ,lean 'itille'
>ater-(K+)
15- T/e tep i repeate' eer. min"te for
15 min"te "ntil t/e mi&t"re top t"rnin%
l"e la,6 in ,olo"r >/en tete' >it/ io'ine
ol"tion-
11- Measure and record t/e time ta6en for
t/e l"e la,6 ,olo"r of io'ine to 'iappear .
using a stopwatch in t/e tale-(K#)1$- Step +011 are repeate' >it/ tet t"e
B3C3 an' ;-(K*)
1*- Ta"late t/e 'ata in t/e tale
elo>(K1)
11- A %rap/ of t/e rate of am.lae a,tiit. a%ain
temperat"re i plotte'-(K#)
+7LPA
Ram"tan
;ifferent
,on,entration of
",roe ol"tion
Peele' ram"tan
7/25/2019 CHECKLIST PAPER 3 BIOLOGY.doc
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#-# STUDYING THE EFFECT OF>H ON PEPSIN ACTI(ITY
AIM:To ineti%ate t/e effe,t of pH on pepin
a,tiit.-
HYPOTHESIS:An a,i'i, me'i"m at pH * i optim"m
for t/e a,tiit. of en.me-
MATERIALS&APPARATUS:Al"min "penion3 14 pepin3
5-173/.'ro,/lori, a,i'3 5-1 7o'i"m/.'ro&i'e ol"tion an' 'itille' >ater-3
Tet t"e3 ea6er3 t/ermometer3 +ml
.rin%e3 pH paper 3a >ire %a"e
Stop>at,/3 'ropper3 a B"nen "rner3 a
tripo' an' a tet0 t"e ra,6-
DIAGRAM:
78 R8 C8CO9CRETE pH of
me'i"m
(a,i'0 pH*3
ne"tral0
pH@3 an'
al6aline0
pH)
T/e ,on'ition
of t/e mi&t"re
at t/e e%innin%
an' after $5
min"te t/e
,larit. of t/e
ol"tion
8ol"me of
al"min
"penion
(+ml)3ol"me
(1ml) an' (14)
,on,entration of
pepin ol"tion
an' temperat"re
of me'i"m
(*@C)
ABSTRACT 0 Rate of rea,tion
,atal.e' .pepin- Pepin
a,tiit.
7ETHO;S
Oin% ol"tion into ea,/ tet
t"e:-(K1)(K*)P : 1 ml of 5-1 7 /.'ro,/lori, a,i' 1 ml of
14 pepin ol"tion 3
F : 1 ml of 'itille' >ater 1 ml of 14
pepin ol"tion 3
R: 1 ml of 5-1 7 of o'i"m /.'ro&i'e 1 ml
of 14pepin ol"tion 3#- ;ip a pie,e of pH paper into ea,/ tet -(K1)
+- T/e tet t"e are immere' in >ater at/ at
*@C temperat"re for $5 min"te-(K$)
?- Observe and record t/e ,on'ition of t/e
mi&t"re at t/e e%innin% an' after $5 min"te
(K#)
@- Calculate and recordt/e rate of reaction
,atal.e' . pepin . "in% t/eformula
! / time-
R-/:Te
t
t"
e
pH 7i&t"re Rate of
rea,tion
,atal.e'
. pep
(min01)-
At t/ee%inni
n%
After$5
min"t
e
P *
F @
R
#-+ STUDYING THE EFFECT OFSUBSTRATE
CONCENTRATION ON
SALI(ARY AMYLASE
ACTI(ITY
"2##%1 EFFECT OF
CONCENTRATION OF
ALBUMIN SUSPENSION ON
THE RATE OF REACTION OF
PEPSIN ENYME'
AIM:To ineti%ate t/e effe,t of "trate
,on,entration on aliar. am.lae
a,tiit.-
HYPOTHESIS:T/e /i%/er t/e concentration of
substrate3 t/e /i%/er t/e rate of reaction
catalysed by salivary amylase "ntil it
rea,/e t/e ma&im"m rate-
MATERIALS&APPARATUS:
5-14 Saliar. Am.lae ol"tion3
'ifferent ,on,entration of tar,/
"penion
(5-1435-$435-*435-#43 5-+4 an' 5-?4)
Io'ine ol"tion3 ;itille' >ater3
Tet t"e ea6erD Stop>at,/3 'ropper3
>/ite tile >it/ %rooe3 Gla ro'
DIAGRAM:
78 R8 C8CO9CRETE Con,entrati
on of
tar,/
"penion
(5-1435-$4
35-*435-#4
3 5-+4 an'
5-?4)
T/e time ta6en
for t/e l"e
la,6 ,olo"r of
io'ine to
'iappear (min)
Temperat"re
(*@C)
ol"me ($ml)
an' (5-14)
,on,entration of
aliar. am.lae
ol"tion
ABSTRACT 0 Rate of rea,tion
,atal.e' .
aliar.
am.lae-
7ETHO;S
O>*
"7'
R*/ 5 7 *)//
"71'
5-143
5-$4
5-*4
1- Laelle'1$tet t"e a A13 A$3 B13B$3 C13
C$ 3;13 ;$3 E13 E$3 in% tet
t"e . "in% .rin%e-(K1)(K*)
A1: 5-14 tar,/ "penion 3
B1: 5-$4 tar,/ "penion3
C1: 5-*4 tar,/ "penion3
;1: 5-#4 tar,/ "penion3
E1: 5-+4 tar,/ "penion3
ater at/ at
temperat"re *@C for + min"te-(K1)(K$)+- ;rop of io'ine ol"tion are a''e' eparatel.
onto t/e %rooe of a >/ite tile "in% a
.rin%e-(K1)
?- After + min"te3 po"r tar,/ "penion (A1)
to am.lae ol"tion (A$) (K1)
@- Stirre' t/e mi&t"re . "in% t/e %la ro' (K1
- Stop>at,/ i a,tiate' imme'iatel. (5
min"te)-(K+)
- A 'rop of mi&t"re i tete' >it/ io'ine
ol"tion on t/e >/ite tile- At eer. amplin%
t/e 'ropper m"t e rine' >it/ ,lean 'itille'
>ater-(K+)
15- T/e tep i repeate' eer. min"te for 15
min"te "ntil t/e mi&t"re top t"rnin% l"e
la,6 in ,olo"r >/en tete' >it/ io'ine
ol"tion-
11- Measure and record t/e time ta6en for
t/e l"e la,6 ,olo"r of io'ine to 'iappear .
using a stopwatchin t/e tale-(K#)(K1)1$- Step +011 are repeate' >it/ tet t"e
B3C3 ;3 E an'
7/25/2019 CHECKLIST PAPER 3 BIOLOGY.doc
5/14
5-#4
5-+4
5-?4
? ?-1"SPM
2##%
1'
DETERMINING THE ENERGY
(ALUE IN FOOD SAMPLES
AIM:To 'etermine an' ,ompare t/e ener%.
,ontent in >/ite rea' an' pean"t-
HYPOTHESIS:Pean"t pro'",e a lot of /eat ener%.
/ite rea' pro'",e alittle /eat ener%.Pean"t pro'",e a
/i%/er in,reain% in temperat"re ener%.
al"e /8*>/ite rea'-
MATERIALS&APPARATUS:;itille' >ater3 a pean"t3
rea'3plati,ine3 an' ,otton >ool-
Boilin% t"e3 t/ermometer3 retort tan'3
a pin3 mea"rin% ,.lin'er3 B"nen
"rner an' ele,troni, alan,e-
DIAGRAM:
78 R8 C8CO9CRETE ater
temperat"re .
"in% t/e
thermometer
Fixed t/e
'itan,e
et>een t/e
oilin% t"e an'
foo' ample
>/i,/ i $,m .
"in% t/eruler
*R("TECHNI
UE'
calculate and
record t/e
ener%. ,ontent in
foo' ample .
"in% t/e
form"lae elo>
Energy value =
Mass of$ater%g& '
%(.#)g *!+C*!& '
increase in
,emperature%C)
Mass of food
%g&-!
=nit (6%01)
Tale:
!. ei%/ t/e pean"t an' re,or' it
>ei%/t(K1)#. @ /8 >*-/ 57 */ /8
6 5 /8 > */).
0. ater
melon
MATERIALS&APPARATUS:
;CPIP Sol"tion3 5-14 aori, a,i'
it/ nee'le3 Spe,imen
t"e
DIAGRAM:
78 R8 C8CO9CRETE t.pe of fr"it
(apple3
oran%e3 an'
>atermelon
)
T/e ol"me of
fr"it "i,e t/at
'e,olo"rie' t/e
;CPIP ol"tion
8ol"me,on,ent
ration of ;CPIP
ABSTRACT 0 per,enta%e of
itamin C
7ETHO;S
Oater
($MC)3
/Con,entrationof ,aron
'io&i'e
ABSTR ACT li%/t
intenit.
Rate of
p/oto.nt/ei
0
7ETHO;S
Oit/ a /arp
raor la'e "n'er >ater- "K1'"K)
#- T/e tran' of"ydrilla p- i pla,e' ini'
a %la filter f"nnel."K1'
+- T/e f"nnel i pla,e' "pi'e 'o>n in a +55ml ea6er."K1'
?- T/e ea6er i fille' >it/ #55 ml of 14
o'i"m i,aronate."K2'
@- T/e ea6er i pla,e' at a 'itan,e of +5 ,m
from t/e ?5 "l a a li%/t o"r,e ."K3'- T/e n"mer of %a "le releae' in on
min"te are ,o"nte' an' re,or'e' in a tale
"K4'- T/i tep i repeate' t>i,e ."K'- Step @ i repeate' . pla,in% t/e apparat" a
'itan,e #5 ,m3 *5 ,m3 $5 ,m an' 15 ,mfrom t/e li%/t o"r,e."K3'
15-T/e re"lt are re,or'e' in a tale"K1'11-T/e %rap/ of t/e rate of p/oto.nt/ei
a%aint t/e li%/t o"r,e i plotte'- "K1'
R-/:;itan,e of
li%/t
o"r,e-
(,m)
9o of %a
"le
releae'
T/e rate of
p/oto.nt/e
i
(min01)
+5
#5
*5
$5
15
? ?-11 IN(ESTIGATING THEEFFECT OF CARBONDIOXIDE CONCENTRATION
ON THE RATE OF
PHOTOSYNTHESIS
AIM:To t"'. t/e effe,t of ,aron 'io&i'e
,on,entration on t/e rate of
p/oto.nt/ei-
PROBLEM STATEMENT:
;oe of ,aron 'io&i'e ,on,entration
affe,t t/e rate of p/oto.nt/ei
HYPOTHESIS:A/en t/e ,on,entration of ,aron
'io&i'e in,reae t/e rate of
p/oto.nt/ei alo in,reae "ntil t/e
rate e,ome ,ontant
MATERIALS&APPARATUS:"ydrillaplant3 'ifferent ,on,entration of
o'i"m /.'ro%en i,aronate (5-$435-#43 5-?4 an' 14)3 plati,ine3
?5 ele,tri, "l3 +55 ml ea6er3 a
oilin% t"e3 top >at,/3 raor la'e3
t/ermometer3 meter r"ler
DIAGRAM:
78 R8 C8CO9CRETE Con,entrati
on of
,aron
'io&i'e
9"mer of %a
"le t/at are
releae in 1
min"te-
Temperat"re of
t/e >ater
($MC)3 /Li%/tintenit.
ABSTRACT 0 rate of
p/oto.nt/ei
0
7ETHO;S
O/i,/ are
5-$43
5-#43 5-?4
an' 14
)R("TECHNI
UE'Count and
record t/en"mer of %a
"le t/at are
releae in 1
min"te . "in%
a stopwatch.
Fixed t/e
'itan,e of
"ydllra p- to
o"r,e of li%/tto 15 ,m .
"in% t/e r"ler-
*R("TECHNI
UE'calculate and
record the rate
of
p/oto.nt/ei
. "in% t/e
form"lae:
no- of "le
releae'
time(min01)
K10 Ho> to et "p t/e apparat" C/ooe 15 ,m len%t/ of fre
"ydrilla sp.
T/e tran' of "ydrilla p- i
pla,e' ini'e a oilin% t"e
Clip t/e tip >it/ a paper ,lip an' p"
it in t/e oilin% >it/ t/e ,lip 'o>n
T/e %rap/ of t/e rate o
p/oto.nt/ei a%aint t/e ,aro
'io&i'e ,on,entration i plotte'
K$0 Ho> to operate t/e ,ontant ariale
Po"r #5 ml of 14 o'i"m
i,aronate ol"tion into t/e oilin%
t"e-
Pla,e t/e apparat" at a fi& 'itan
from a li%/t o"r,e >/i,/ i 15 ,m
K# Ho> to operate t/e repon'in% ariale
Co"nt an' re,or' t/e n"mer o
"le releae' in + min"te T/e rate of p/oto.nt/ei i
,al,"late' . "in% t/e form"lae:
(n"mer of "letime)
K* Ho> to operate t/e manip"late
ariale
Repeat K1 to K# "in% 'ifferen
per,enta%e of o'i"m i,aronat
ol"tion >/i,/ are 5-$43 5-#4
5-?4
K+ 0 Pre,a"tion
Pla,e t/e oilin% t"e in a ea6er o
>ater to maintain t/e temperat"re
R-/:Con,entrati
on of
9o of %a
"le
T/e rate of
p/oto.nt/e
7/25/2019 CHECKLIST PAPER 3 BIOLOGY.doc
7/14
,aron
'io&i'e (4)
releae' in
one min"te
i (min01)
1-5
5-?
5-#
5-$
@ @-1 STUDYING THE PROCESS OFAEROBIC RESPIRATION
AIM:To t"'. t/e pro,e of aeroi,
repiration
HYPOTHESIS:T/e aeroi, repiration pro'",e ,aron
'io&i'e to in,reae t/e /ei%/t of
,olo"re' liN"i'-
MATERIALS&APPARATUS:ater3 ,olo"re' liN"i'3 a ,o,6roa,/ an'
o'a lime3 oilin% t"e3 +55ml ea6er3
$+5ml ea6er3 ,apillar. t"e3 ,re>
,lip3 an' a >ire %a"e-
DIAGRAM:
78 R8 C8CO9CRETE Preen,e of
,o,6roa,/
Hei%/t of
,olo"re' liN"i'-
Temperat"re of
t/e >ater at/
(*@MC)3 amo"nt
of o'a lime-
ABSTRACT 0 Pro,e of
aeroi,
repiration
0
7ETHO;S
Oater at/
>/i,/ i *@MC
. "in% a
t/ermometer
*R("TECHNI
UE'
1- Lael t>o oilin% t"e >it/ A an' B(K1)
$- ater an'
temperat"re
Temperat"re of
t/e >ater at/
(*@MC)3 ol"me
of %l",oe
ol"tion( 1+ ml)38ol"me of lime
>ater($ml)
ABSTRACT 0 pro,e of
aeroi,repiration
0
7ETHO;S
Oater at/
>/i,/ i *@MC
. "in% a
t/ermometer
*/- "C'
L7
o oilin% t"e >it/ A an' B(K1)
*-
7/25/2019 CHECKLIST PAPER 3 BIOLOGY.doc
8/14
@ @-$"SLG
RJ #'
IN(ESTIGATING THE
DIFFERENCES BET?EEN
THE INHALED AND
EXHALED AIR IN TERMS OF
OXYGEN AND CARBON
DIOXIDE CONTENTS
AIM:To 'etermine t/e o&.%en an' ,aron
'io&i'e ,ontent in in/ale' an' e&/ale'
air
PROBLEM STATEMENT:
;oe in/ale' air ,ontain more o&.%enan' le ,aron 'io&i'e t/an e&/ale'
air
HYPOTHESIS:I8*6 *,ontain more o&.%en *6
le ,aron 'io&i'e t/an e&/ale' air
I8*6 *,ontain more ,aron
'io&i'e *6le o&.%en t/an e&/ale' air
E8*6 *,ontain more ,aron
'io&i'e *6le o&.%en t/an in/ale' air
0E8*6 *,ontain more o&.%en *6le ,aron 'io&i'e t/an in/ale' air-
MATERIALS&APPARATUS:
Potai"m /.'ro&i'e ol"tion3
Potai"m p.ro%allate ol"tion
3ater3 0t"e3 R"ler3 Bea6er 3 Boilin%
t"e 3 Bain >ater at/3 R"er t"in%
DIAGRAM:
78 R8 C8CO9CRETE 0t/e len%t/ of air
,ol"mn o,,"pie'
. o&.%en in
in/ale'e&/ale'
air
0 t/e len%t/ of
air ,ol"mn
o,,"pie' .
,aron 'io&i'e
in
in/ale'e&/ale'
air
0T8 /*
;/8 5 *
)-7(Same)
t"'ent
0t"e
;iameter of 0
t"eCon,entrat
ion of KOH
Temperat"re
Time to ,olle,t
air ampleAir
ampleABSTRACT In/ale' air
an' e&/ale'
air
per,enta%eN"ant
it. of o&.%en
an' ,aron
'io&i'e iin/ale'
an' e&/ale' air
0
7ETHO;S
Oa ,/oen a t/e fiel' t"'.-
"K1'
$- F"a'rat of ie 1m & 1m >a "e'-
"K2'"K1'
*- T>o plant pe,ie pe,ie A an' B >a
i'entifie'."K1' "K3'#- T/e N"a'rat >ere t/ro>n at ran'om in t/e
,/ool fiel' "K1'+- T/e area of (,oera%e) ea,/ plant pe,ie
pe,ie A an' pe,ie B >a ,o"nte'- "K4'?- T/e n"mer of in'ii'"al plant pe,ie in
ea,/ N"a'rat >a ,o"nte'-"K4'@- If more t/an /alf of t/e N"are in t/e
N"a'rat i ,oere'3 t/e area of plant pe,ie
P)/*; 5 O2 ! _(y z)_cm 1##=
x cm
& len%t/ of air ,ol"mn of in/ale'e&/ale' air
. len%t/ of air ,ol"mn "pon a''in% porai"m
P)/*; 5 CO2 ! _(x y)_cm 1##=
x cm
& len%t/ of air ,ol"mn of in/ale' e&/ale' air
7/25/2019 CHECKLIST PAPER 3 BIOLOGY.doc
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pe,ie A an' B in t/e ,/ool fiel'
$ ;oe t/e t.pe of plant pe,ie affe,t
t/e per,enta%e ,oera%e pop"lation
ie 'enit. of t/e plant
*- /i,/ t.pe of t/e plant pe,ie
pe,ie A or B /a t/e /i%/et
per,enta%e ,oera%e pop"lation ie
HYPOTHESIS:1- T/e per,enta%e ,oera%e
pop"lation ie of pe,ie A plant i
/i%/er t/an pe,ie B in t/e ,/ool
fiel'-$- ;ifferent plant pe,ie /ae 'ifferent
per,enta%e ,oera%e pop"lation ie -
*- Plant pe,ie A i more 'ominant
t/an pe,ie B in t/i /aitat-
MATERIALS&APPARATUSPlant pe,ie A an' B an. $ plant
peie Plati, N"a'rat3 mar6er pen3 A#
Paper3 %rap/ paper-
DIAGRAM:
plant
;enit. of
pe,ie
7ETHO;S
O/i,/ are
pe,ie A
an' pe,ie
B
)R("TECHNI
UE'
Measure and
recordt/e area
of ea,/ t.pe of
pe,ie "in% a
N"a'rat 1m & 1m
Fixed t/e ie of
N"a'rat to
1m1m
*R("TECHNIUE'
6efer to the
sentences below.
M/86 *R(Calculate and record t/e per,enta%e ,oera%e of plant
pe,ie A an' B "in% the formulae
Total area ,oere' . t/e pe,ie Q 1554
9"mer of N"a'rat Q area of one N"a'rat
Cal,"late t/e 'enit. of plant pe,ie "in% t/e form"la:
Total n"mer of or%anim in all N"a'rat
9"mer of N"a'rat Q area of one N"a'rat
R-/
>ill e ,o"nte' - T/e area i not ,o"nte' if
onl. le t/an /alf i ,oere'-"K'
- Step + to @ >a repeate' for nine N"a'rat-
"K1'
- T/e area ,oere' . plant pe,ie pe,ie A
an' pe,ie B n"mer of in'ii'"al plant
pe,ie t"'ie' in ea,/ N"a'rat >erere,or'e' an' ta"late' in a tale- "K4'
15- T/e per,enta%e ,oera%e 'enit.
freN"en,. of plant pe,ie pe,ie A an'
pe,ie B >ere ,al,"late' . "in% t/i
form"la: "K4'
11- P)/*; )*; 5 >*/ >) :
Total area ,oere' plant pe,ie in all
N"a'rat Q 1554
Total n"mer of N"a'rat Q area of a N"a'rat
F-) 5 >)
9"mer of N"a'rat ,ontainin% plant pe,ie Q
1554
Total n"mer of N"a'rat
1$- D/ 5 >*/ >)
total n"mer of in'ii'"al pe,ie in all
N"a'rat
Total n"mer of N"a'rat Q area of a N"a'rat
-? ESTIMATING THEPOPULATION SIE OF
GARDEN SNAILS USING
CAPTURE, MARK, RELEASE
AND RECAPTURE
TECHNIUE
AIM:To etimate t/e pop"lation ie of
%ar'en nail "in% ,apt"re3 mar63
releae an' re,apt"re te,/niN"e-
PROBLEM STATEMENT:
/at i t/e effe,t of 'ifferent ie of
ample on t/e ie of %ar'en nailpop"lation
HYPOTHESIS:T/e lar%er t/e ie of ample3 t/e more
a,,"rate of t/e nail pop"lation
etimate'-
MATERIALS&APPARATUSSnail3a paintr"/3a ottle of In'ian
in63 a pen an' a noteoo6-
DIAGRAM:
78 R8 C8CO9CRETE T/e ie of
ample
0 T/e ie of
reear,/ area
ABSTRACT 0 t/e %ar'en nail
pop"lation
0
7ETHO;S
O/i,/ are
mall an'
lar%e
ample
)R("TECHNI
UE'
0
Fixed t/e ie of
reear,/ area to
1+m1+m
*R("TECHNI
UE'calculate and
record t/e t/e
%ar'en nailpop"lation .
"in% t/e
form"lae:
Pop"lation ie
% a - b& 7 c
a T/e n"mer
of nail
in t/e firt
,apt"re
n"mer of
nail
in t/e e,on',apt"re
, no of mar6e'
ir' in e,on'
,apt"re-
R-/
9"mer of nail
Sie of %ar'en nail
pop"lation
Sample of
firt ,apt"re
Se,on' ,apt"re
Total
n"mer
7ar6e'
nail
1t
ample
1*- Sele,t a "itale lo,ation >it/in .o"r ,/ool
,ompo"n' >it/ t/e area 1+m1+m ."K1'
"K2'1#- Capt"re a man. %ar'en nail a .o" ,an
from t/e pla,e-"K1'
1+- Co"nt t/e %ar'en nail t/at .o" /ae
,apt"re' an' mar6 t/eir /ell >it/ a mall
'ot of In'ian in6- "K4'"K'1?- Releae t/em in t/e ame pla,e >/ere .o"
fo"n' t/em "K1'1@- Go a,6 to t/e ame pla,e after een 'a.-
Capt"re on,e a%ain a man. %ar'en nail a
.o" ,an-
1- Co"nt t/e total n"mer of %ar'en nail .o"
/ae ,apt"re' an' note t/e n"mer of t/oe>/i,/ /a' een mar6e'."K4'
1- Re,or' t/e 'ata in a tale "K1'
$5- Calculate and record t/e pop"lation ie of
%ar'en nail "in% t/e follo>in% form"la:
Pop"lation ie % a - b& 7 c
a8 ,he number of snailsin the first capture
b8 number of snails
in the second capture
c8 no of mar9ed snails in second
capture1*- Repeat t/e tep $ to . "in% t/e lar%er
n"mer of nail ample-"K3'
1#- Re,or' t/e 'ata in t/e tale- "K1'
7/25/2019 CHECKLIST PAPER 3 BIOLOGY.doc
10/14
$n'
ample
-@"SBP
#0'
IN(ESTIGATING THE
EFFECT OF A CHANGE IN PH
"ABIOTIC FACTOR' ON THE
POPULATION GRO?TH
RATE OF AN ORGANISMAIM:To ineti%ate t/e effe,t of ,/an%e in pH
al"e on t/e pop"lation %ro>t/ rate of
:emnap- plant-
PROBLEM STATEMENT:
1- ;oe t/e ,/an%e in pH al"e affe,t
t/e pop"lation %ro>t/ rate of
:emnap plant
$- /at i t/e effe,t of ,/an%e in pH
al"e on t/e pop"lation %ro>t/ rate
of:emnap-
*- /i,/ pH al"e i t/e mot "itale
for t/e in,reae in pop"lation of
:emnap-
HYPOTHESIS:1- T/e pH @ i t/e mot "itale for t/e
in,reae in pop"lation of:emnap-
plant ,ompare' to pH $ an' pH 1#-$- T/e pop"lation %ro>t/ rate of
:emnap- plant i t/e /i%/et in
t/e pH @ ,ompare' to pH $ an' pH
1#-
MATERIALS&APPARATUS:
:emnap- plant3 D'itille' >ater 'il"te
/.'ro,/lori, a,i' o'i"m /.'ro&i'e
ol"tion3 ,"lt"re ol"tion pon' >ater-
Bea6er petri 'i/ ,ontainer3
mea"rin% ,.lin'er3 pH paper meter-
DIAGRAM:
78 R8 C8CO9CRETE T.pe o f
ol"tion
"e'
T/e in,reae in
pop"lation of
:emnap-
plant-
Spe,ie of
:emnap-
plant ol"me
of >ater ,"lt"reol"tion
,on,entration of
n"trient
temperat"re
li%/t intenit.
time
ABSTRACT ;ifferent
pH al"e
pH $3 pH @
an' pH 1#
T/e pop"lation
%ro>t/ rate of
:emnap-
plant-
0
7ETHO;S
Ot/ rate of
:emnap-
plant ('a.01)
;a.01 ;a.0+In,reae
;e,reae
9e"tral
A,i'i,
Al6ali
1- C/ooe:emna p- plant of t/e ame ie-
"K1'"K2'$- C/ooe ta6e t/ree petri 'i/e of t/e am
ie."K1'
*- Lael t/e petri 'i/e a A3 B an' C."K1'#- Po"r + ml of 'itille' >ater into petri 'i/
A3 + ml of /.'ro,/lori, a,i' into petri 'i/
B an' + ml of o'i"m /.'ro&i'e ol"tion
into petri 'i/ C- "K1'"K3'
+- Tet t/e pH al"e of ea,/ ol"tion "in% pH
paper (an' re,or' in a tale)-"K1'
?- Po"r + ml of ,"lt"re ol"tion pon' >ater
into ea,/ petri 'i/-"K1'"K2'
@- P"t +:emna sp.plant into ea,/ petri 'i/-
"K1'
- Re,or' in a tale."K1'- Pla,e t/e petri 'i/e on t/e tale near t/e
>in'o> in t/e laorator.."K2'15-C/an%e t/e ol"tion in t/e petri 'i/e eer.
'a.-"K'11-Co"nt t/e n"mer of:emna sp- plant after
+ 'a.-"K4'1$-Cal,"late t/e pop"lation %ro>t/ rate of
:emna sp.plantusing a formula "K4'
T/e pop"lation %ro>t/ rate of Lemna p-
9"mer of:emna sp + 'a.
1*-Repeat t/e e&periment tep 1 "ntil 11 to
%et t/e a,,"rate re"lt- "K'
14.T*9-*/ /8 6*/* /8 /*9 9at,/3 >ater at/3 t/ermometer-
DIAGRAM:
78 R8 C8
CO9CRETE ;ifferenttemperat"re
of >ater
at/
Hei%/t of t/e,olo"re' liN"i'
in t/e
manometer
8ol"me of .eat"penion
ABSTRACT 0 0
7ETHO;S
Oater poll"tion
at lo,ation P F R
HYPOTHESIS:T/e >ater at lo,ation R i more poll"te'
,ompare' to lo,ation P an' lo,ation F-
T/e >ater at lo,ation R i t/e motpoll"te' ,ompare' to lo,ation P an'
lo,ation F T/e met/.lene l"e ol"tion
too6 t/e /ortet time to 'e,olo"rie in
ample >ater R ,ompare' to ample
>ater F an' P-
MATERIALS&APPARATUS:7et/.lene l"e ol"tion (5-14)3 >ater
ample3 top >at,/3 rea%ent ottle3
.rin%e >it/ nee'le3 mea"rin% ,.lin'er-
DIAGRAM:
78 R8 C8CO9CRETE Lo,ation of
>ater
Time ta6en to
'e,olo"rie
met/.lene l"e
ol"tion
8ol"me of >ater
ample
(155ml)
ol"me of
met/.lene l"e
ol"tion (1ml)
,on,entration of
met/.lene l"eol"tion
ABSTRACT 0 Leel of >aterpoll"tion 0
7ETHO;S
Oater
ample
from t/e
lo,ation P3
F3 an' R
)R("TECHNI
UE'Measure and
record time to
'e,olo"rie
met/.lene l"e
ol"tion . "in%
a stopwatch
Fixed t/e
ol"me of
met/.lene l"e
ol"tion >/i,/
i 1 ml by using
a measuring
cylinder.
*R("TECHNI
UE'*
R-/:
Rea%e
ntottle
ater
ample
Time ta6en to
'e,olo"riemet/.lene
l"e ol"tion (/)
Leel of >ater
poll"tion(/01)
A P
B F
C R
1- ater ample are ,olle,te' from P3F an' R
"K1'$- T/e rea%ent ottle are laelle' A3B3C- "K1'
*- 7ea"re 1## 7 5 from P3F
an' R eparatel. an' po"r into t/e rea%ent
ottle laelle' A3B an' C repe,tiel.- "K2'
"K3'#- 1 7 5 7/8 9- -/i a''e' to
t/e ae of ea,/ >ater ample "in% a
.rin%e-"K2'
+- T/e rea%ent ottle are ,loe' >it/ t/etopper imme'iatel.."K1' "K'
?- T/e ,ontent of t/e ottle ,annot e /a6en
"K'@- All t/e rea%ent ottle are 6ept in a 'ar6
,"poar' "K'- T/e top>at,/ i a,tiate'."K1'
- T/e ottle are e&amine' from time to time.
"K1'
15-T/e time ta6en for t/e met/.lene l"e
ol"tion to 'e,olo"rie e,ome ,olo"rle
i re,or'e' for all t/e >ater ample."K4'11-T/e re"lt are re,or'e' in a tale- "K1'
15 15-@ SHO?ING XYLEM AS ACONTINUOUS TUBE SYSTEM
THAT TRANSPORTS ?ATER
AND MINERALS
AIM:To /o> t/at &.lem a a ,ontin"o" t"e
,an tranport >ater an' mineral-
PROBLEM STATEMENT:;oe Q.lem ti"e form a ,ontin"o"
t"e .tem t/at tranport >ater an'
mineral from t/e root to t/e /ootHYPOTHESIS:Q.lem ti"e form a ,ontin"o" t"e
.tem t/at tranport >ater an'
mineral from t/e root to t/e /oot-
Q.lem ti"e ,an tranport >ater an'
mineral-
MATERIALS&APPARATUS:A alam plant3 eoin ol"tion3 ea6er3
raor la'e3 mi,ro,ope li'e3 ,oer
lip3 a mi,ro,ope3 for,ep3 a >/ite tile3
petri 'i/ an' a paintr"/-
DIAGRAM:
78 R8 C8CO9CRETE Part of
plant at
leae3 tem
an' root
T/e ti"e
taine' re' >it/
t/e re' ,olor of
eoin-
T.pe of plant
ABSTRACT 0 Part of plant
ti"e t/at ,an
tranport >ater
an' mineral-
0
7ETHO;S
O/i,/
are at leaf3
tem an'
root
)R("TECHNI
UE'
Observe and
record t/e
ti"e taine'
re' >it/ t/e re'
,olor of eoin .
"in% a
microscope.
Used the same
t.pe of plant
>/i,/ i t/e
Balam plant-
*R("TECHNI
UE'*
1- T/e root of Balam plant i >a/e' "K1'
"K2'$- T/e root i immere' in eoine ol"tion for
*5 min"te- (K1)
*- /en t/e re' eoin ol"tion /a penetrate'
into t/e ein of t/e leae3 t/e plant i
remoe'- (K+)
#- T/in e,tion of t/e tem are ,"t >it/ raor
la'e-(K1)
+- A paintr"/ i "e' to tranfer a t/in ,ro
e,tion of t/e tem onto a 'rop of >ater on a%la li'e-(K1)
?- T/e e,tion i ,oere' >it/ a ,oer lip an'
e&amine' "n'er a mi,ro,ope-(K1)
@- T/e ti"e >/i,/ /a een taine' >it/ t/e
re' ,olo"r of eoin i i'entifie'(K#)
- Step #0? are repeate' >it/ t/e ,ro e,tion
of root an' leaf-(K*)
- ;ia%ram of ,ro e,tion of t/e root3 tem
an' leaf "n'er lo> po>er i 'ra>n an'in'i,ate' t/e part taine' re'-(K#)
R-/:Part of plant Cro e,tion of
plant
Leaf
Stem
7/25/2019 CHECKLIST PAPER 3 BIOLOGY.doc
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Root
15 15-* CARRYING OUT BARKRINGING TO SHO? THE
ROLE OF PHLOEM IN THE
CONTINUOUS TRANSPORT
OF ORGANIC SUBSTANCES.
AIM:
To /o> t/e role of p/loem in t/e,ontin"o" tranport of or%ani,
"tan,e-
PROBLEM STATEMENT:
1-/at i t/e effe,t of remoin% a rin%
of p/loem ti"e from t/e tem of a tree
$- ;oe t/e remoin% a rin% of p/loemti"e from t/e tem of a tree >ill affe,t
t/e tranportation of or%ani, "tan,e
HYPOTHESIS:T/e rin%e' tem /o> ti"e aoe t/e
rin% >ell3 >/erea t/e ti"e elo>
t/e rin% ten' to >it/er-
MATERIALS&APPARATUS:
S/arp 6nife3 Healt/. /ii,"
tree38aelin an' mea"rin% tape-
DIAGRAM:
78 R8 C8CO9CRETE A tem t/at
i rin%e'
an' a tem
t/at i not
rin%e'
T/e ,on'ition
aoe an' elo>
t/e rin% after one
mont/
T/e 'iameter
of t/e temaoe an' elo>
t/e rin% after one
mont/-
Con'ition of
enironment3
t.pe of plant3
time of t/e
e&periment
ABSTRACT 0 Part of plant
ti"e t/at ,an
tranport or%ani,
"tan,e-
0
7ETHO;S
O t/e rin%
after one mont/
. "in%ameasuring tape.
Used the same
t.pe of plant>/i,/ i t/e
Hii," plant-
*R("TECHNI
UE'*
1- T>o tree tem of /ii," plant are ,/ooen
an' laelle' A an' B-(K1) (K$)
$- A 6nife i "e' to remoe a ,omplete rin% o
ar6 from a tree tem A- (K1)
*- 8aeline i applie' on t/e e&poe' ti"e-
(K1)(K+)
#- ;ra> t/e ,on'ition of tem -Measure andrecord initial 'iameter of t/e tem aoe
an' elo> t/e rin% . "in% a measuring
tape.(K#)(K1)
+- After 7/83 t/e ,on'ition of t/e rin%e'
tem aoe an' elo> t/e rin% are oere'
an' re,or'e'-( K#)(K$)
?- A 'ra>in% of t/e tem ,on'ition i
'ra>n-Measure and record t/e 'iameter of
t/e tem aoe an' elo> t/e rin% after one
mont/ . "in% a measuring tape.(K#)
@- Repeat tep #3 + an' ? . "in% tem B for
not rin%e' tem-(K*)
- T/e ,on'ition of t/e tem i ,ompare' to t/e
tem t/at i not rin%e'-(K#)
R-/:T.pe of
tem
;iameter
(,m)
,on'ition
Before one
mont/
;iameter
(,m)
,on'ition
After one
mont/
A Rin%e'
Stem
B 9ot
Rin%e'
tem
15 15- STUDYING THE EFFECT OFAIR MO(EMENT ON THE
RATE OF TRANSPIRATION
BY USING POTOMETER.
AIM:To t"'. t/e effe,t of air moement on
t/e rate of tranpiration-
PROBLEM STATEMENT:
/at are t/e effe,t of t/e tate 'ifferentpee' of air moement on t/e rate of
tranpiration
HYPOTHESIS:A t/e pee' of t/e air moement
in,reae3 t/e rate of tranpiration
in,reae
T/e rate of tranpiration i /i%/er in a
moin% air t/an in a till air
MATERIALS&APPARATUS:Capillar. t"e3 r"er t"e3 top >at,/3
r"ler3 ea6er3 fan3 retort tan' >it/
,lamp3 raor la'e3 ain3 mar6er3 Plant
/oot3 >ater3 aeline
DIAGRAM:
78 R8 C8CO9CRETE State
pee' of air
moement
Time ta6en for
air "le to
trael from P to
F (min)
Con'ition of
enironment3
t.pe of plant3
ABSTRACT 0 Rate of
tranpiration
0
7ETHO;S
O/i,/ i t/e
Hii," plant-
/ Fixedt/e
temperat"re of
"rro"n'in% to
*@MC . using
the
thermometer.
*R("TECHNI
UE'Calculate and
record t/e rate of
tranpiration .
"in% t/e
form"lae of
distance
travelled dividedby time ta9en
R-/:
State of
air
moem
entSpe
e' of
t/e fan
Time ta6en for air "le to trael from Q
to Y3 (min)
Rate of
tranpir
ation
min01
1 1 $ * Aera%e
$
1. C8 *6 )-/ 55 * *5 8/ 57 *
89)- >*/."K1'"K2'
2. I77 /8 )-/ 6 776*/ / *
9* 5 it/ t/e ot/er en' of t/e ,apillar.
t"e immere in a ea6er of >ater (K1)
$. ipe 'r. t/e leae an' apparat" "in% 'r.
,lot/
0. Smear all t/e oint of t/e apparat" >it/
aeline to preent lea6a%e (K+)
1#.Pla,e t/e apparat" "n'er t/e fan >/i,/ i
>it,/e' off-
11.Intro'",e an air "le into t/e ,apillar.
t"e . liftin% t/e en' of t/e ,apillar. t"eo"t of t/e ea6er for a /ort >/i le an' t/en
ret"rne' it to t/e ea6er a%ain (K1)
12.Allo> t/e air "le to moe "ntil it rea,/et/e P mar6 an' a,tiate t/e top >at,/
13.Stop t/e top >at,/ >/en t/e air "le
rea,/e t/e F mar6
14.Measure and record the time ta6en for air
"le to trael from P to Fby using a
stopwatchin t/e tale proi'e' (K#) (K1)
1.Repeat tep 1to 1# to otain an aera%e
rea'in%
1.Repeat t/e aoe tep . >it,/in% on t/e
fan "in% 'ifferent pee' of t/e fan (pee'
13 $3 *3 #3 +) an' re,or' t/e re"lt (K*)
K1 : P>**/ 5 >)7 *6 *>>**/- %
>/7/ "*/ */ 4S / ;/ * /)@'
7/25/2019 CHECKLIST PAPER 3 BIOLOGY.doc
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*
#
+
S1: C/ooe a leaf. /oot
S$: C"t t/e tem oliN"el.
S*: Inert t/e r"er t"e into ,apillar. t"e
S#: Inert t/e ,"t tem to t/e r"er t"e
S+: Set "p t/e apparat" in "pri%/t poition
S?: Intro'",e t/e air "le
K3 : H*6; /8 7*>-*/6 **9
it,/ on an' fan >it,/ on
K2 : H*6; /8 )/6 **9
C/ooe an' ,"t off a leaf. /oot from a /ii,"
plant-
K4 : H*6; >6; **9 %C)/; *6 )6; 6*/*
0 a,tiate t/e top >at,/ at Q
0 top t/e top >at,/ at Y
*Measure and record the time ta6en for air
"le to trael from P to Fby using a
stopwatch
K : A))-*) 5 /8 6*/* 9/*6 %
P)*-/0 repeat t/e tep an' fin' t/e aera%e
0 mear >it/ aeline to preent lea6a%e >ipe
'r. t/e leae an' apparat"
1$ 1$-1 STUDYING THE EFFECT OFDIFFERENT UANTITIES OF
?ATER INTAKE TO THE
URINE OUTPUT
AIM:
To t"'. t/e effe,t of 'ifferent N"antitieof >ater inta6e ol"me of 'rin6 on t/e
ol"me of "rine o"tp"t-
PROBLEM STATEMENT:
/at i t/e effe,t of 'ifferent N"antitie
of >ater inta6e on t/e ol"me of "rine
o"tp"t
HYPOTHESIS:T/e more >ater (78) 'r"n63 t/e more
t/e ol"me of "rine o"tp"t (R8) i
forme'-
T/e /i%/er t/e N"antit. of >ater inta6e
(78)3 t/e more t/e ol"me of "rine
o"tp"t (R8) i forme'-
MATERIALS&APPARATUS:St"'ent3 paper ,"p3 'rin6in% >ater3
mea"rin% ,.lin'er-
DIAGRAM:
78 R8 C8CO9CRETE 8ol"me of
'rin6
F"antit. of
>ater
inta6e .t"'ent-
8ol"me of "rine
pro'",e
Enironmental
,on'ition
(temperat"re3
/"mi'it.)
Gen'er3 ie an'a%e of t"'ent
ABSTRACT 0
7ETHO;S
Oater3
t"'ent B *55 ml of mineral >ater3 t"'ent C
#55 ml of mineral >ater an' t"'ent ; +55
ml of mineral >ater to 'rin6-(K1)(K*)
#- Measure and recordt/e ol"me of "rine
o"tp"t of t/e t"'ent >it/in t/at /o"r-(K#)
+- T/e "rine pro'",e' i ,olle,te' in paper
,"p an' mea"re' into mea"rin% ,.lin'er
(K1)
?- Re,or' t/e ol"me of "rine pro'",e' .
ea,/ t"'ent in tale-(K1)
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1+ 1+-1 IN(ESTIGATING (ARIATIONIN HUMANS
AIM:To ineti%ate t/e t.pe of
ariation(78) amon% t"'ent(R8) in +
a"/ari-
To t"'. t/e n"mer of t"'ent(R8)
>it/ 'ifferent /ei%/t an' t.pe of
fin%erprint(78)
PROBLEM STATEMENT:1- ;o all t/e t"'ent /ae t/e ame
t.pe of fin%erprint an' /ei%/t t.pe
of ariation$- ;o 'ifferent t.pe of fin%erprint an'
/ei%/t affe,t t/e n"mer of t"'ent
HYPOTHESIS:;ifferent -79 5 /-6/(R8)
/o> 655/ /> 5 5;>/
*6 8;8/ "M(' % /> 5 **/
inerel.
MATERIALS&APPARATUS:
1' S/-6/ M
$) Grap/ paper 0 7
*) A# paper >/ite
paper 0 7
#) ti"e paper ,lot/ 0
7
+) /i,/are 1@ .ear ol'-
*R("TECHNI
UE'*
R-/:
St"'ent2
name
T.pe of fin%er print Hei%/t
"7'>/orl C"re Compoite loop
1-
$-
*-
#-
+-
?-
@-
-
-
15
@- Ten name of t"'ent in t/ ame a%e >ere
>ritten 'o>n in a tale (K1) (K$)
- T/e /ei%/t i mea"re' . "in% a metre
r"ler an' re,or'e' in a tale- (K1) (K#)
- T/ e&periment i repeate' . ineti%atin%
t/ t.pe of fin%erprint( K*)15-B. "in% a fin%erprint pa'3 pla,e' t/e
t/"mprint on a >/ite papert>i,e- ( K1)
11-B. "in% a /an' len3 t/ t.pe of t/"m prin
>ere oere' an' i'entif.- (K#)
1$-Step $ "ntil + >ere repeate' to ot/er
t"'ent in t/ ame %ro"p- (K*)1*-T/ mea"rement of /ei%/t an' fin%erprint
are repeate' t>i,e to %et t/ aera%e- (K+)
1#-T>o %rap/ on t/ n"mer of t"'ent
a%aint t/ t.pe of ariation >ere plotte'-
(K1)