CHARACTERIZATION OF NATURAL AND ENGINEERED NANOPARTICLES: SHAPE, SIZE

AND CHEMICAL COMPOSITIONLucia Manangon

http://www.theismaili.org/cms/317/Building-the-future-one-nanoparticle-at-a-time

NANOPARTICLES

Ferrihydrite

Natural nanoparticle

CNT

0.25 µm

100 nm

TEM image of 20 nm Au NP

Engineered nanoparticle

Poda et al., J. Chromatogr. A. 2010, 1218 (27), 4219-4225.

NANOPARTICLES IN COMERCIAL PRODUCTS

FATE ??BEHAVIOR ??

BIOAVAILABILITY ??TOXICITY ??

Characterization and Analytical Techniques

SizeShape

Composition

• Field Flow fractionation (FFF)

• Multi Angle Light Scattering (MALS)

• Dynamic Light Scattering (DLS)

• Inductively coupled Plasma (FFF-ICP-MS)

FFF

FRACTIONATION

MULTI ANGLE LIGHT SCATTERING (MALS) – DYNAMIC LIGHT SCATTERING (DLS)

incident beam

scattered light

SIZE AND SHAPE

ICP-CC-MS

Li

C

Na

Mg

Al

Si

P

S

K

Ca

Ti

Mn

Fe

Co

Ni

Cu

Zn

As

Se

Sr

Mo

Ag

Sb

Cs

Hg

Pb

U

27 elements are analyzed:

CHEMICAL COMPOSITION

Online system

ICP - MS

FFF

UV

MALS - DLSFLUORESCENCE

SAMPLES

Au, AgPS microspheresCNT

Natural nanoparticle

Engineered nanoparticle

GSLPore water Deep brine layerDeep brine sediment

FRACTIONATION SIZE AND SHAPE CHEMICAL COMPOSITION

UNDERSTANDING OF FATE, BEHAVIOR, BIOAVAILABILITY AND TOXICITY