CBX2-FLAG

CBX4-FLAG

CBX6-FLAG

CBX7-FLAGCBX8-FLAG

Additional file 1: Figure S1

A

B

76 kDa –

52 kDa –

38 kDa –

31 kDa –

24 kDa –

++

++

+

FLAG IP

220kDa–

76kDa –

HPH1-FLAG

HPH2-FLAG

HPH3-FLAG

++

++

FLAG IP

pcDNA6

-FLAG

-CBX6(MRO19)

-CBX7(Abcam)

-CBX8(Bethyl)

-FLAG

-HPH1(MRO25)

-HPH2(1615)

-HPH3(MRO27)

GFPRING1-FLAGRING2-FLAG

RING1

RING2

RING2

RING1

-RING1

RING2

C + ++ +

+ +

FLAG IP: + + +–––

76 kDa –

52 kDa –

38 kDa –

FLAG

76 kDa –

52 kDa –

38 kDa –

-C

BX

6

-C

BX

4

-C

BX

7-

CB

X8

Inpu

t

-C

BX

6

-C

BX

4

-C

BX

7-

CB

X8

Inpu

t

CBX6CBX4

D

– Ig