Post on 02-Apr-2018
7/27/2019 CBB4032 Chemical Analysis Introduction
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Course outline
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Subject : CBB4032 Chemical Analysis
Credit hour: 2
Semester : Sem 2 Yr 4
Status: Major subject
Prerequisite: No
Sypnosis:
Introduction to various types of equipments, Sampling
Method, Gravimetry, Spectrophotometry, Spectroscopy,
Chromatography, Application procedure and interpretation
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1. Apply knowledge of mathematics, science and engineering fundamentals and an engineering
specialisation to the solution of complex chemical engineering problems.
2. Identify, formulate, research literature and analyse complex chemical engineering problemsreaching substantiated conclusions using first principles of mathematics, natural sciences and
engineering sciences3. Design solutions for complex chemical engineering problems and design systems, components or processes
that meet specified needs with appropriate consideration for public health and safety, cultural, societal, and
environmental considerations.
4. Investigate complex chemical engineering problems using research based knowledge and research methods
including design of experiments, analysis and interpretation of data and synthesis of information to providevalid conclusions.
5. Use modern engineering and IT tools to evaluate complex chemical engineering activities.
6. Apply reasoning informed by contextual knowledge to assess societal, health, safety, legal and cultural issues
and the consequent responsibilities relevant to professional engineering practice.
7. Understand the impact of professional engineering solutions in societal and environmental contexts and
demonstrate knowledge of and need for sustainable development.
8. Apply ethical principles and commit to professional ethics and responsibilities and norms of chemical
engineering practice9. Communicate effectively on complex chemical engineering activities with the engineering community and
society.
10. Function effectively as an individual, and as a member or leader in diverse teams and in multi-disciplinary
settings.
11. Recognise the need for, and have the preparation and ability to engage in independent and life-long learning in
the broadest context of technological change.
12. Demonstrate knowledge and understanding of engineering and management principles and apply these toones own work, as a member and leader in a team, to manage projects and in multidisciplinary environments.
Chemical Engineering Programme Outcomes (PO)
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Course outline
Course Learning Outcome (CLO):
At the end of this course, students should be able to
CO1: Analyze data obtained from specific analytical
equipment. Map to PO1/PO2
CO2: To interpret the generated data from specific
analytical equipment. Map to PO1/PO2
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Course outline
5
Course
Planning
Topic Hours
Lect. Tut. Lab
Introduction 3
Gravimetry 4 1 #
Spectrophotometry 4 1 #
Spectroscopy 9 2 #
Chromatographic Method 8 2 #
Laboratory Work 8
Total Hours28 6 8
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Course content
1.0 Introduction
Analytical Objectives
Qualitative Analysis
Quantitative Analysis
Type of equipment/
apparatus - Gravimetry,
Spectrophotometry,
Spectroscopy andChromatography
2.0 Gravimetry
Gravimetry Calculations
Properties of Precipitates
Colloidal Precipitates
Crystalline Precipitates
Co-precipitation
Homogeneous Solution
Precipitate Drying
Summary of Methods
Application
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Course content
3.0 Spectrophotometry
Electromagnetic Radiation
Absorption Process
Qualitative Analysis
Quantitative Analysis
Measuring Absorbance
Emission Process
4.1 Spectroscopy
Atomic Spectroscopy
General Consideration
Atomic Emission
Atomic Absorption
Atomic Fluorescence
Use of X-rays
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Course content
4.2 Spectroscopy
Molecular Spectroscopy
Organic UV-Vis Absorption
Effect of Solvent
Inorganic UV-Vis Absorption
Qualitative Analysis
Quantitative Analysis
Spectrophotometric Titrations
Molecular Fluorescence
Equipment for MolecularSpectroscopy
5. Chromatography
Principle of Chromatography
Classifications ofChromatoraphic Techniques
Qualitative Analysis Quantitative Analysis
Gas ChromatographyPrinciples and Instrumentation
Liquid Chromatography
Principles and Instrumentation Paper Chromatography and
Thin Layer Chromatography
Hyphenated Methods (GCMS,HPLC, GC-FTIR)
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Grading
Quiz/ Test/ Assignment - 30%
Laboratory/ Project - 20%
Final Examination - 50%
Laboratory Work
Students are required to conduct any four experiments underthe following subjects:
Gravimetry
Fourier Transform Infrared Spectroscopy UV-Visible Spectroscopy
Gas Chromatography/GCMS
High Performance Liquid Chromatography
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Text book
Gary D. Christian, 2003 Analytical Chemistry, 6th Ed., Wiley
Skoog, West, Holler and Crouch, Analytical Chemistry AnIntroduction, 7th Edition, Saunders, ISBN 0-03-020293-0.
Christie G. Enke, 2001. The art and Science of ChemicalAnalysis, Wiley Higher Education. ISBN: 978-0-471-37369-8.
Francis Rouessac and Annick Rouessac, 2005. Chemical Analysis:Modern Instrumentation Methods and Techniques, 2nd Edition,
Wiley Higher Education. ISBN: 978-0-470-85903-2
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Timetable
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Previous Week Printer Friendly Next WeekBack To Selection Wed May 15 14:38:19 2013
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1.0 Introduction
Geranyl acetate
O
CH
Everything is made of chemicals
Elements, substance, molecules
Analytical chemist determinewhat and how much
Investigates, inspect, analyze
Sample characterization for allaspects of material
Analytical chemistry is science
of chemical measurements
12Geraniol
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Objective: Areas in Chemical analysis
Question
Quantitation
Detection
Identification
Separation
Answer
How many substance in
sample? ----------
Does the sample contain Y?--------------
What is the identity of the
substance? ---------
How can interested analytebe separated from sample
matrix? -----------
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What do chemical analyst do?
Analyst
Applies known
measurement techniques to
a well defined
compositional orcharacterization questions
Senior analyst
Develop a new method of
measurement on the
existing principles to solve
new problem
Research analytical chemist
Create or investigate noveltechniques for chemicalmeasurement
Or conduct fundamentalstudies of chemical/physicalphenomena underlyingchemical measurement
Provides methods and tools needed forinsight into material to answer the 4Qsabout a sample:
What
Where
How much
What appearance - structure & form
What is analytical chemistry?
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Qualitative vs quantitative analysis
Qualitative analysisprovides info(identification) about
the identity ofspecies or functionalgroup in the sample
Quantitative analysisprovides numericalinfo of analyte (exactamount orconcentration)
Qualitative analysis is what.
Quantitative analysis is how much.
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Measurement science
Progress in measurement science involve thebasic and targeted research
Research
Targeted
Innovativeconcepts , new
principles
Characteriz
ationanalysis
Specific problem ,novel applications ofexisting techniques
Basic
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Measurement science
Provide tools and eyes to scientist and engineers
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Analytical techniques: Method precision,
sensitivity, selectivity and capability Different method provide different precision,
sensitivity, selectivity and speed capabilities
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Analytical method
Classical methods: Wet chemical methodssuch as precipitation, extraction, distillation,boiling or melting points, gravimetric andtitrimetric measurements.
Instrumental methods: Analyticalmeasurements (conductivity, electrodepotential, light absorption or emission, mass-
to-charge ratio, fluorescence etc.) are madeusing instrumentation.
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Analytical methods
Gravimetric methods determine the mass of the analyte orsome compoundchemically related to it.
Volumetric method, the volume of a solution containingsufficient reagent to react completely with the analyte is
measured.
Electroanalytical methods involve the measurement of suchelectricalproperties as voltage, current, resistance, and quantityof electrical charge.
Spectroscopic methods are based on measurement of theinteraction between electromagnetic radiation and analyteatoms or molecules or on the production of such radiation byanalyte.
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Analytical methodology
Plan
Qualitative & quantitative or both
What kind of info Which technique
Sampling
Accuracy depends on proper sampling
Characteristic of sample is very important
Require good representative sample
Sampleprep
Depends on analytical technique
Type of sample
Analyticalmeasure
ment
The equipments
Data
analysis
The valid data range, replication of analysis
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Consideration in selecting
equipment
Accuracy
Sample amount
Concentration range
Interference
Physical/chemical properties of sample matrix
Number of sample to be analyzed
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Selecting
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Selecting
analytical
methods
An analysisinvolves several
steps and
operations which
depend on:
the particular
problem
your expertise
the apparatus or
equipment
available.
The analyst should
be involved in every
step.
Gary Christian,
Analytical Chemistry,
6th Ed. (Wiley)23
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Established standard
Used established and well recognized
standard or protocol for analysis e.g.:
ASTM
USEPA
BSI
SIRIM
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ASTM
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USEPA
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BSI
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SIRIM
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Summary:
Focus areas
Function of chemical analyst
Qualitative and quantitative
Measurement science
Analytical method
Recognized standard
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