FIBROBLAST MICRO RNAS REGULATE TUMOR CELL AND P53 INDUCE TRANSCRIPTIONAL REPRESSION

LUISA FERNANDA GUARIN HINCAPIE

MEDICINE STUDENT – UPB – 2011

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INTRODUCCTION

The nucleus in a eukaryotic cell is the

most important part; it controls several activities of the

cell, like the replication of

DNA, transcription and processing of

RNA.The eukaryotic cells

have a exclusive gene expression, that

is why the ARNm suffer

posttranscriptional process before been

transported from nucleus to

cytoplasm.1

MICRO RNAs ARE INVADING THE TUMOR MICROENVIRONMENT:FIBROBLAST MICRO RNAs REGULATE TUMOR CELL MOTILITY ANDINVASIVENESS. Cell Cycle (Dic 7-2010)

MICRO RNAs ARE INVADING THE TUMOR MICROENVIRONMENT:FIBROBLAST MICRO RNAs REGULATE TUMOR CELL MOTILITY ANDINVASIVENESS. Cell Cycle (Dic 7-2010)

Malignant tumor cellsinteract all the timewith stroma elements,they control tumorprogressionspreading, andresponse to therapy;cancer associatedfibroblasts (CAFs)participate at allstages of tumorgrowth andprogression

MICRO RNAs ARE INVADING THE TUMOR MICROENVIRONMENT:FIBROBLAST MICRO RNAs REGULATE TUMOR CELL MOTILITY ANDINVASIVENESS. Cell Cycle (Dic 7-2010)

But CAFs are notnormal fibroblasts;they stimulate tumorcells by secretingMMPs, cytokines andgrowth factors. Studiessuggest that thealteration geneexpression is the resultof mechanisms pre-existing or induce bytumor cell inflammationor specific tumor tissueconditions.

MICRO RNAs ARE INVADING THE TUMOR MICROENVIRONMENT:FIBROBLAST MICRO RNAs REGULATE TUMOR CELL MOTILITY ANDINVASIVENESS. Cell Cycle (Dic 7-2010)

Micro RNAs is necessary for a normal development even in cancer; miRNAs repress expression of targeting genes by inhibiting them translation or inducing mRNA degradation. Now we know that the unique role of CAFs in humans is tumor cells motility.

PERSONAL OPINION

Now that we know about CAFs I think we should study

more about which factors cause damages to

miRNAs in CAFs to prevent metastatic

cancer

P53, TRANSCRIPTIONAL REPRESSION AND DRUGSENSITIVITY: FRESH PERSPECTIVES ON AN OLDACTIVITY Cell Cycle (Dic 7-2010)

P53, TRANSCRIPTIONAL REPRESSION AND DRUGSENSITIVITY: FRESH PERSPECTIVES ON AN OLDACTIVITY Cell Cycle (Dic 7-2010)

P53 tumor suppressor becomesas a transcription factor aftersuffer cellular stress (DNAdamage, hypoxia, etc.); now p53can be a transcriptional activatoror transcriptional repressor ofgene expression.

P53, TRANSCRIPTIONAL REPRESSION AND DRUGSENSITIVITY: FRESH PERSPECTIVES ON AN OLDACTIVITY Cell Cycle (Dic 7-2010)

p53can induce cellcycle arrest andapoptosis. Thetranscriptionalrepression by p53 ofthe gene encodingPolo like kinases(PLK1) and Cdc25c,both participate in thecycle cell checkpointand belong toCDE/CHR elements(cell cycle dependentelement/cell cyclegenes homologyregion).

P53, TRANSCRIPTIONAL REPRESSION AND DRUGSENSITIVITY: FRESH PERSPECTIVES ON AN OLDACTIVITY Cell Cycle (Dic 7-2010)

PLK1 andCdc25c aretypicallyrepressed in non-cycling (normal)cells, and overexpressed intumor cells withmutant p53.

PERSONAL OPINION

Cancer is a very complicated situation to

patients that is why is so important to detect it on time and studies like

that give us hope

MEDICAL UTILITY

MEDICAL UTILITY

MICRO RNAs ARE INVADING THE TUMOR MICROENVIRONMENT:FIBROBLAST MICRO RNAs REGULATE TUMOR CELL MOTILITY ANDINVASIVENESS. Cell Cycle (Dic 7-2010)

When a patient has cancer is

important tray to avoid a metastatic

cancer and the fact to understand

the dynamic is so important, that is

why is so important to go deeper

with CAFs studies.

MEDICAL UTILITY

P53, TRANSCRIPTIONAL REPRESSION AND DRUG SENSITIVITY: FRESH PERSPECTIVES ON AN OLD ACTIVITY

The tumors can be so aggressive with human body,

they should be detected as soon as possible;

technology has contributed with many diagnostic

methods giving us the possibility to save lives.

BIBLIOGRAPHY

MARTINEZ S., Lina María. Biología molecular. 5. ed. Medellín : UPB. Fac. de Medicina, 2009.

Shurin MR. Micro RNAs are invading the tumor microenvironment: Fibroblast micro RNAs regulate tumor cell motility and invasiveness. Cell Cycle. 2010 Dec7;9(22):4430-1. Pub Med PMID: 21088483.

Murphy ME. p53, transcriptional, and drug sensitivity: fresh perspectives on an old activity. Cell Cycle. 2010 Dec7;9(22):4432. Pub Med PMID: 21188768.

LUISA FERNANDA GUARIN

HINCAPIE

THANKS A LOT