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- 1. Extracting DNA fromfruitsExtracting DNA fromfruits
- 2. Verify to have all the materials before starting the
experiment (pic. 1). Melt the salt (about 10 g) in 100 ml of water
(pic. 2). Add a teaspoon (about 10 g) of liquid soap (pic. 3 and
4).
- 3. Stir slowly, without making foam, which may hinder the
contact between the solution and the banana (pic. 5 and 6). Mash
100g of banana (pic. 7 and 8).
- 4. Pour the mush into the solution (pic. 9). Wait for 20
minutes.
- 5. What isWhat is DDNNAA??
- 6. DNA or desoxyribonucleic acid is a molecule content in the
cell nucleus.
- 7. If we could look DNA closely, we would discover that this
molecule is composed by two strands which, joined together, fold up
forming a double-helix similar to a spiral stairs. If we could look
DNA closely, we would discover that this molecule is composed by
two strands which, joined together, fold up forming a double-helix
similar to a spiral stairs.
- 8. The rungs of this stair correspond to chemical substances
called nucleobases which form a chemical bond two by two. DNA can
be compared to an important instruction book which contains genetic
informationuseful for cells to makedifferents chemicals molecules
known as proteins.
- 9. WHO DISCOVERED DNA?WHO DISCOVERED DNA? In 1953, the
scientists J. Watson and F. Crick discovered the double-helix
structure of DNA. They were helped by the
- 10. Unfortunately, Franklin did not recived Nobel Price for
medicine in 1962 with her collegues Watson and Crick because she
died before the assignment of the Prize, but it is necessary
to
- 11. COME BACK TO OURCOME BACK TO OUR
EXPERIMENT!!!EXPERIMENT!!!
- 12. After 20 minutes, filter the mixture (pic. 10-12).
- 13. Wait until the mixture goes through the filter (pic.13 and
14). Take a sample of 5 ml from the filtered mixture with a syringe
(pic. 15 and 16).
- 14. Move the liquid into the test tube, avoiding making foam
(pic. 17). Add 2 ml of pineapple juice using another syringe (pic.
18). Turn upside down the tube 2 or 3 times to facilitate the
mixing of the liquids (pic. 19).
- 15. Add 10 ml of ethyl alcohol (pic. 20 e 21). Wait a few
minutes and the ball of DNA will emerge and, using a magnifying
glass you'll be able to see it. (fig. 22-24).