All Rights Reserved AEIC 2004.

y = - 0.1567 x2 + 1.3795x + 0.06562R = 0.9999

0.0 0.5 1.0 1.5 2.0 2.5

OD 0.1% LOD

Soymeal was toasted for 60 min. at 100 ºC

Antibody not selective for toasted soymeal

Antibody detects toasted soymeal

y = - 0.1567 x2 + 1.3795x + 0.06562R = 0.9999

0.0 0.5 1.0 1.5 2.0 2.5

OD 0.1% LOD

Soymeal was toasted for 60 min. at 100 ºC

Antibody not selective for toasted soymeal

Antibody detects toasted soymeal

Trait Agdia EnviroLogix Neogen SDI

CP4 EPSPS E,S S E1,S

Cry1Ab E,S E,S E,S E2,S

Cry9C E3,S E,S E3,S

Cry2A E,S

Cry3A E

NK603 S S S

www.agdia.com www.envirologix.com www.neogen.com www.sdix.com

E ELISA

S strip1validated by JRC ring test for Roundup Ready Soy bean2validated by AACC ring test for YieldGard (Mon810) Corn3official method AACC international collaborative trial

PCR ELISA Strips

Design DNA/Line specific Protein specific Protein specific

Requirements Sophisticated Moderate Easy

Assay time 3-14 days 2-7 days 5-10 min

Sample cost $400-600 $100+ $7-10

Availability Published/Testing Co Diagnostic Co. Diagnostic Co.

Validation JRC (qualitative) JRC/USDA USDA

Application Qualitative/semi-quant Qual/semi/quant Qual/ test/ compliance with

threshold

Limitations Basic pH, heat, heat, extraction, Same as ELISA false pos, false neg reference materials pro-zone,

dynamic sys Protein dependent 1 sample/test

Sensitivity ~>0.1% GMO ~>0.3% GMO ~1% GMO

All Rights Reserved AEIC 2004.

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