Post on 13-Mar-2018
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ADVANCED PLACEMENT BIOLOGY SYLLABUS
2011-2012
I. Course description:
Advanced Placement Biology is intended to provide an intensive study of
selected topics for those junior or senior students planning on a biologically
related field of study in college. The course content is organized into three units:
molecules and cells, heredity and evolution, and organisms and populations.
Specific content will include: physiology and anatomy of major animal phyla,
genetics, biotechnology, biochemistry, plant systematics, ethology, and ecology
with evolution infused into all of these topics. There are eight overarching themes
that will also be integrated into all lessons and laboratory activities and they are as
follows: Science as a Process, Evolution, Energy and Transfer, Continuity and
Change, Relationship of Structure and Function, Regulation, Interdependence in
Nature, Science Technology and Society. As proposed by the College Board for
2013 and beyond this course will integrate the following four “Big Ideas” as well: 1) The process of evolution drives the diversity and unity of life 2) Biological
systems utilize free energy and molecular building blocks to grow, to reproduce
and to maintain dynamic homeostasis, 3) Living systems store, retrieve, transmit
and respond to information essential to life processes, 4) Biological systems
interact, and these systems and their interactions possess complex properties. The
content in this course will build on previous knowledge from past courses, and
relate concepts to science, technology, and society.
The class will be conducted at the college level and students are expected
to work accordingly. The first 3 days of each week meet for extended time (72
minutes), with the remaining days meeting for the regular class periods of 52
minutes. Students will be expected to come to class prepared and on time. The
overall success of the program depends in large part, on each student meeting
their own responsibilities, with a significant portion of the course content covered
independently by the student. Students will document their course work in the
form of a well organized lecture and laboratory notebook that will be graded at
the end of each quarter. Students will also be required to write scientific papers
each quarter, and prepare PowerPoint presentations.
A comprehensive exam with lab practical will be given each semester, including a combination of objective and essay questions. All unit examinations
are multiple-choice with a topic related essay question. Students are required to
take the AP examination (school will pay examination cost) and attend all classes.
The BioOlympiad Exam will also be given in the winter to every student, a
national exam that identifies and recognizes talented biology students.
II. Laboratory:
More than one third of the course is laboratory. It will include independent
research (developing procedures and executing an experiment(s) with your lab
team), and selected Advanced Placement designed experiments; a comparative
study through the dissections of vertebrate and invertebrate animals; and the
examination of selected specimens representing plants, animals, and protists.
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Some labs will include the use of Labpro, Smartboard®, or laptop computer and
Vernier® probe technology, and laboratory data will be processed using the
department laptop computers and Excel® spreadsheets. All aspects of the course
focus on integrating observed data and relating this information to our society,
and the interactions that we have with the Earth.
III. Scientific paper and portfolio:
A scientific paper will be written each of the first three quarters, and a portfolio
the fourth quarter. The scientific paper will follow the general format used for a
research report published in a scientific journal. The paper will reflect the basic
scientific method of asking a question, formulating a hypothesis, conducting
experiments to test the hypothesis, and interpreting the results. Sections will
include an abstract, introduction, materials and methods, results (data tables and
graphs), discussion, conclusion and works cited. These papers will make
connections between our work in laboratory and the world around us, using
science as a process skill. Each paper will have a point value equivalent to two
test grades. The portfolio will be a compilation of your best work, prepared for
review in an organized notebook. The four sections are as follows: application,
investigation, research entry, and open choice. A rubric will be used to grade
your answers to questions and will evaluate you on depth of understanding,
evidence of inquiry, communication, and relevance to society. The objective of
the portfolio is to reflect upon the concepts that we have discussed throughout the
year, bringing together the common treads of biology with the theme of evolution,
and how science, technology and our society are dependent upon one another.
The portfolio will have a point value equivalent to two test grades.
IV. Text: Campbell, Reece, 2005. Biology, Seventh Edition, Benjamin/Cummings
Publishing.
Student Media, Text CD: Campbell, Reece, 2005. Biology, Seventh Edition,
Version 1.0, Benjamin/Cummings Publishing. Contains: Chapter Guides,
Activities, Investigations, Quizzes, Cumulative Test, Graph It!, Key Terms,
Flashcards.
Lab Manual: AP Biology Lab Manual for Students, 1999, College Board.
Student Study Guide: Preparing For The Biology AP Exam. Pearson Education
Inc., 2005.
Online Text: www.campbellbiology.com.
IV. Topic Outline: (Using: Campbell, Reece, 2005. Biology, Seventh Edition.)
A. Organisms and Populations:
1. Ecology
a. An Introduction to the Biosphere (ch 50) Dissolved O2 Lab
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b. Ecosystems (ch 54) Fertilizer Lab.
c. Population Ecology (ch 52) Human Population Lab.
d. Behavioral Ecology (ch 51) Habitat Selection Lab.
e. Community Ecology (ch 53)
B. Molecules and Cells:
1. Chemistry
a. Chemical Context of Life (ch 2)
b. Water and the Fitness of the Environment (ch 3)
c. Carbon and the Molecular Diversity of Life (ch 4)
d. The Structure and Function of Macromolecules (ch 5)
Macromolecule Lab.
e. A Tour of the Cell (ch 6) Egg Demonstration.
f. Membrane Structure and Function (ch 7) Signal Transduction
Pathway Prelab-Demonstration.
g. An Introduction to Metabolism (ch 8) Enzyme Lab, LabPro
Enzyme Lab, Toothpickase Lab.
h. Cellular Respiration (ch 9) Respiration Lab, glycolysis
demonstration.
i. Photosynthesis (ch 10) Photosynthesis Lab,
Photosynthesis/PH Lab.
j. Cell Communication (ch 11) Signal Transduction Pathway
Lab (optional)w/ soybeans.
k. The Cell Cycle (ch 12) Mitosis Lab.
C. Heredity and Evolution
1. DNA and RNA:
a. Meiosis and Sexual Life Cycles (ch 13) Sordaria Lab.
b. The Molecular Basis of Inheritance (ch 16) DNA replication
demonstration.
c. From Gene to Protein (ch 17)
d. The Genetics of Viruses and Bacteria (ch 18) Bacteria slides
prepared.
e. Eukaryotic Genomes (ch 19)
f. DNA Technology and Genomics (ch 20) Gel Electrophoresis
Lab, Transformation PBLU Lab, Transformation Glow Lab.
g. Mendel and the Gene Idea (ch 14) Drosophilia Lab.
h. The Chromosomal Basis of Inheritance (ch 15)
i. The Genetic Basis of Development (ch 21)
2. Mechanisms of Evolution:
a. Decent with Modification (ch 22)
b. The Origin of Species (ch 24)
c. The Tree of Life (ch 26)
d. The Evolution of Populations (ch 23) Hardy-Weinberg Lab.
e. Phylogeny and Systematics (ch 25)
f. Prokaryotes (ch 27) Bacteria and Cyanobacteria Slides Lab.
A. Continued: Organisms and Populations
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1. Diversity of Organisms.
a. Protists (ch 28) Protist (live) Lab.
b. Plant Diversity (ch 29)
c. Evolution of Seed Plants
d. Fungi (ch 31)
2. Structure and Function of Plants and Animals
a. Plant Structure, Growth, and Development (ch 35)
Root, Stem, and Leaf Lab, Grocery Store Botany Lab.
b. Transport in Vascular Plants (ch 36) Transpiration Lab
c. Plant Nutrition (ch 37)
d. Angiosperm Reproduction and Biotechnology (ch 38)
e. Plant Responses to Internal and External Signals
(ch 39) Hormone Lab
f. Introduction to Animal Diversity (ch 32) Animal
Diversity Lab I.
g. Invertebrates (ch 33) Animal Diversity Lab II.
h. Vertebrates (ch 34)
i. Basic Principles of Animal Form and Function (ch 40)
j. Animal Nutrition (ch 41)
k. Circulation and Gas Exchange (ch 42) Circulation Lab,
EKG Lab, Q10 Goldfish Lab.
l. Immune System (ch 43)
m. Osmoregulation and Excretion (ch 44)
n. Hormones and the Endocrine System (ch 45)
o. Animal Development (ch 47)
p. Nervous System (ch 48) Nervous Tissue and Reflex
Arc, Eye Dissection Lab
q. Sensory and Motor Mechanisms (ch 49)
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CURRICULUM MAP 2011-2012
SEMSTER I
Summer, August, September October November, December,January
Content 50,52,54,51,53-10% Ecology and Biosphere
Behavioral Biology Population Biology
Community Biology
Ecosystems
2,3,4,5,-7%
Chemical Context of Life
Water and Fitness
Carbon and Life
Macromolecules
8, Introduction to Metabolism
6,7,11,12,-10% Tour of Cell
Membrane Structure Cell Communication
Cell Cycle
9,-8%
Cellular Respiration
10-(continued) 8% Photosynthesis
13,16,17,18,19,20-9% Meiosis/Sexual Life Cycles
Molecular Basis/ Inheritance
Gene to Protein
DNA Technology
Control of Eukaryotic Genomes
Genetics of Bacteria/Viruses
Skills Online Cricket Lab
Biome Log
Dissolved O2 Productivity Lab
Fertilizer Runoff/ DO Lab Human Population Studies
Fish Lab
Animal Behavior Lab Cell Races, Designing a Cell
*Biuret/Proteins Lab (optional)
*Sudan III/Fat Lab (optional)
Carbo-unknown Lab
Toothpickase
Enzyme Catalysis Lab
Labpro Enzyme Action Lab
*Strip Mine Field Trip Water
Sampling (optional) Cell Modeling Activity
Area/Volume/Mass Properties of water demonstration
Diffusion Osmosis Lab
Egg Demonstration
Mitosis Lab
Cell Respiration Lab
Glycolsis Demo
*Signal Tranduction Pathway Lab
(optional) Signal Transduction Demo
Design a Plasma Membrane
Photosynthesis Demo
LabproPhotosynthesis/PH Lab
Pigments/Photosynthesis Lab
Meiosis Lab
Sordaria Genetics Lab DNA Replication Demo
Drosophila Genetics Lab
Restriction Enzyme/Electrophoresis Lab
Paper Plasmids Restriction Analysis PP Lab
Bacteria prepared slides.
Assessment Lecture Notebook, Labs, Scientific Paper, Quizzes, Essays, Exams.
Lecture Notebook, Labs, Quizzes, Exams.
Lecture Notebook, Labs, Scientific Paper, Quizzes, Exams.
%= Percentage of Course. Any component of this map may change at anytime. This curriculum map is under
construction!!!!
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CURRICULUM MAP 2011-2012
SEMSTER II
January, February March April, May, June Content 14,15-9% Cont.
Mendel and Genes
Chromosomal Inheritance
21-9% Cont.
Development 22,24,26-8%
Darwinian view, Species
Origin, Tree of Life 23,25,27,28-8%
Population Evolution, Phylogeny,
Prokaryotic/Eukaryotic Diversity 29,30,35-32% Plants
Plant diversity, Seed evolution,
Structure/growth
36,37,38,39-32%Cont.
Plant Transport, Nutrition,
Reproduction-development, Control systems
32, 33, 34-8% Animal Evolution,
Invertebrates, Vertebrate
Evolution
40,41,42,43,44-32% Cont. Animal structure/function,
Nutrition, Circulation, Defenses,
Internal environment
45,47,48,49-32% Cont.
Chemical signals, Development,
Nervous system, Sensory/motor
April Exam Review
May Exam Review
Exam Date May 9th at 8:00am
Skills Genetics problem sets Transformation Lab
Glow/Gene Lab
*Protein Gel Lab (optional)
Hardy-Weinberg Root, Stem, Leaf Lab
Hardy-Weinberg Problems
Bacteria/ Cyanobacteria Slides Protist (live/prepared) slides
Grocery Store Botany Lab
Development-interactive (9 month miracle)
Plant Hormone Lab
Transpiration Lab
Body systems-interactive “Parade Through The Kingdoms”
Animal Diversity I, II Lab
Body systems-interactive 5.0 Physiology / Circulation Lab
Q10 Goldfish Lab.
Nervous Tissue Lab
Review! Fetal Pig Dissection
EKG Lab/ *Cow Heart Dissection (optional)
Nobel Powerpoint after Exam
Assessment Lecture Notebook, Labs, Quizzes, Essays, Exams.
Lecture Notebook, Labs, Quizzes, Exams.
Lecture Notebook, Labs, Pig practical, Quizzes, Exams.
%= Percentage of Course. Any component of this map may change at anytime. This curriculum map is under construction!!!!
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MAJOR THEMES
I. Science as a process: Science is a way of knowing. It can involve a
discovery process using inductive reasoning, or it can be a process of
hypothesis testing.
II. Evolution: Evolution is the biological change of organisms that occurs over
time and is driven by the process of natural selection. Evolution accounts for
the diversity of life on Earth.
III. Energy Transfer: Energy is the capacity to do work. All living organisms are
active (living) because of their abilities to link energy reactions to the
biochemical reactions that take place within their cells.
IV. Continuity and change: All species tend to maintain themselves from
generation to generation using the same genetic code. However, there are
genetic mechanisms that lead to change over time, or evolution.
V. Relationship of Structure to Function: The structural levels from molecules to
organism ensure successful functioning in all living organisms and living
systems.
VI. Regulation: Everything from cells to organisms to ecosystems is in a state of
dynamic balance that must be controlled by positive or negative feedback
mechanisms.
VII. Interdependence in Nature: Living organisms rarely exist alone in nature.
VIII. Science, Technology, and Society: Scientific research often leads to
technological advances that can have positive or negative impacts upon society as a whole.
Source: CollegeBoard AP Biology Course Description, May 2006, May 2007.
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COURSE-LONG PLAN BY TOPIC, CHAPTER, THEME, SKILLS, AND
ASSESSMENTS
MONTH: August/ September.
UNIT: Organisms and Populations.
TOPIC: Ecology. CHAPTERS:
50- Ecology and Biosphere. Themes: 1,3,4,5,6,7,8 Skills: PP=Paper Pencil Biome Logs.
Describe the relationship between ecology and evolutionary biology.
51-Behavioral Biology. Themes:1,2,4,5,6,7,8 Skills: CB=College Board Habitat Selection Lab.
Describe how genes and environment contribute to behavior.
52-Population Biology. Themes: 2,3,4,5,6,7,8 Skills: PP Fish Lab, PP Human Population
Lab.
Describe the characteristics of populations, life histories, growth and
limiting factors.
53-Community Biology.Themes:2,3,4,5,6,7,8.
Describe how individuals interact with one another, other communities
and the environment.
54- Ecosystems. Themes: 2,3,4,5,6,7,8 Skills: CB Dissolved O2 Lab, *Inquiry-based DO
Fertilizer Lab.
Explain the interactions between ecosystems, energy and matter and the
environment.
Assignments and assessments: Read all chapters, lab grades, ecology essay and exam,
scientific paper from fertilizer lab.
UNIT: Molecules and Cells.
TOPIC: The Chemistry of Life. CHAPTERS:
2-The Chemical Context of Life. Themes: 3,5,6,8.
Describe the biological application of elements, compounds, atoms and
molecules.
3-Water and Fitness. Themes: 1,3,5,6,8.
Describe the properties of water. *Properties of water demonstration.
4-Carbon and Molecular Diversity. Themes: 3,5,6,8.
Explain the importance of carbon and various functional groups in
biology. 5-Structure/Function of Macromolecules. Themes: 3,4,5,6,8 Skills: *Carbohydrate Lab.
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Assignments and assessments: Read all chapters, lab grade, water essay (from summer
reading) and macromolecule exam.
Describe the structure and function of macromolecules and their
significance to the diversity of life.
UNIT: Molecules and Cells.
TOPIC: The Cell. CHAPTERS:
8-An Introduction to Metabolism. Themes: 3,4,5,6,8 Skills: CB Enzyme Catalysis,
*Labpro Enzyme Action, PP Toothpickase.
Assignments and assessments: Read chapter, lab grades, enzyme essay and metabolism
exam.
Describe how metabolism, energy, and life are all interconnected.
MONTH: October.
UNIT: Molecules and Cells.
TOPIC: The Cell (continued) CHAPTERS (continued):
6-Tour of the Cell. Themes:1,2,3,4,5,6,7,8.
Compare and contrast eukaryotic and prokaryotic cells.
Explain how cell complexity coincides with evolutionary change.
* Modeling limits to Cell Size, Cell Races Lab 7-Membrane Structure and Function. Themes: 1,3,5,6,8 Skills: CB Diffusion/Osmosis
lab, Egg Demonstration, Plasma Membrane Activity.
Describe the basic structure and function of cell membranes.
11-Cell Communication. Themes: 3,5,6,8.
Describe the process of signal transduction pathways.
12-Cell Cycle. Themes: 3,5,6,8 CB Mitosis Lab.
Explain the role of cell division in reproduction, growth, and repair.
9-Cellular Respiration. Themes: 3,4,5,6,8 Skills: CB Cellular Respiration lab, PP
Glycolysis Demonstration, Signal Transduction Demonstration.
Compare and contrast cellular respiration with fermentation and the
evolutionary significance of this advancement.
Assignments and assessments: Read all chapters, cell and membrane exam with
diffusion essay, cellular respiration exam and essay.
MONTH: November, December, January.
UNIT: Molecules and Cells.
TOPIC: The Cell (continued) CHAPTERS (continued)
10-Photosynthesis. Themes: 3,5,6 Skills: CB Photosynthesis Lab, *Labpro
Photosynthesis/PH probe Lab.
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Explain the process of photosynthesis and the adaptations some land
plants exhibit to survive in extreme environments.
Assignments and assessments: Read chapter, photosynthesis exam and essay, scientific
paper over photosynthesis/ ph lab, and lab grade.
UNIT: Heredity and Evolution.
TOPIC: Genetics. CHAPTERS
13-Meiosis. Themes: 1,2,4,5,6,7,8 Skills: Sordaria Lab CB Meiosis.
Explain the role of meiosis in sexual life cycles, and the origins of genetic
variation.
16-Molecular Basis of Inheritance. Themes: 1,2,4,5,6,8 Skills: DNA Demonstration, CB Drosophila Lab.
Describe DNA as heritable material, and how it replicates and repairs
itself. 17-Gene to Protein. Themes: 1,2,3,4,5,6,8.
Explain the process of synthesizing RNA and proteins from DNA.
20-DNA Technology. Themes: 1,2,4,5,6,8 Skills: CB Restriction Enzyme/
Electrophoresis Lab, PP Paper plasmids, PP Restriction Analysis.
Describe the many facets of biotechnology, such as PCR, cloning, GMO’s,
Human Genome Project, etc.. 19-Eukaryotic Genomes. Themes: 1,2,4,5,6,8.
Compare and contrast genomic expression in prokaryotes and eukaryotes.
18-Genetics of Viruses and Bacteria. Themes: 1,2,4,5,6,8.
Explain the reproductive cycle of viruses and bacteria and how they
evolved.
Assignments and assessments: Read all chapters, meiosis and inheritance exam, all other
chapters covered on semester 1 exam, lab grades.
SEMESTER I EXAM AND LABORATORY PRACTICAL (Includes all labs for
semester 1)
MONTH: January and February.
UNIT: Heredity and Evolution.
TOPIC: Genetics (continued) CHAPTERS
14-Mendel and the Gene. Themes: 1,2,4,5,6,8 Skills: PP Genetics Problems CB.
Transformation Lab, *Transformation Glow-in-the-Dark gene.
Distinguish between Mendelian genetics and Non-Mendelian genetics.
15-Chromosomal Basis of Inheritance. Themes: 1,2,4,5,6,8 Skills: PP Genetics Problems.
Explain the chromosome theory of inheritance.
21-Genetic Basis of Development. Themes: 1,5,6,8.
Describe differential gene expression and the genetic and cellular
mechanisms of pattern formation.
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Assignments and assessments: Read all chapters, genetic problem sets, lab grade,
genetics exam.
UNIT: Heredity and Evolution.
TOPIC: Evolution.
CHAPTERS 22-Descent with Modification. Themes: 1,2,4,5,6,8.
Describe the historical context for evolutionary theory.
24-Origin of Species. Themes: 1,2,3.
Explain the species concept model and modes of speciation.
26-Tree of Life. Themes: 1,2,3.
Describe the hypothesis for the origin of life and the history of life.
23-Evolution of Populations. Themes: 1,2,4,7,8 Skills: PP Hardy-Weinberg Problems,
CB Hardy-Weinberg Lab.
Compare and contrast evolving and nonevolving populations through
population genetics. 25-Phylogeny and Systematics. Themes: 1,2,4.
Describe how phylogenies are based on common ancestries and how we
connect classification with evolutionary history.
Assignments and assessments: Read all chapters, Hardy-Weinberg problems, evolution
exam and essay.
UNIT: Organisms and Populations.
TOPIC: Evolution/ Biological Diversity. CHAPTERS
27-Prokaryotes. Themes: 1,4,5,6,7,8 Skills: *Bacteria/Cyanobacteria (live) prepared
slides lab.
Explain the structure and function of prokaryotes and their significance in
the evolution of Earth.
28-Protists. Themes: 2,4,5,7 Skills: *Protist (live/prepared) Slides Lab
Identify the diversity of Protists as a group and explain why they no longer
fit the kingdom approach to classification. 29/30-Plant Diversity. Themes: 1,2,4,5,7.
Describe the various groups of plants and their evolution.
Assignments and assessments: Read all chapters, lab grade, Botany Exam I.
UNIT: Organisms and Populations.
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TOPIC: Plant Form and Function. CHAPTERS
35-Plant Structure/Growth/Development. Themes: 2,3,4,5,6 Skills: *Root/Stem/Leaf Lab,
*Grocery Store Botany- Modifications.
Identify the characteristics of plant structure and function.
Assignments and assessments: Read chapter, lab grade.
MONTH: March
UNIT: Organisms and Populations.
TOPIC: Plant Form and Function (continued)
CHAPTERS 36-Transport in Vascular Plants. Themes: 3,5,6 Skills: CB Transpiration Lab.
Describe the transport mechanisms found in plants.
37-Plant Nutrition. Themes: 3,5,6.
Describe the nutritional needs of plants and their adaptations.
38-Angiosperm Reproduction/Biotechnology. Themes: 3,5,6.
Compare and contrast sexual and asexual reproduction in plants.
39-Plant Responses. Themes: 3,5,6 Skills *Plant Hormone Lab.
Distinguish between the various types of plant responses
Assignments and assessments: Read chapters, labs, Botany Exam II.
UNIT: Organisms and Populations.
TOPIC: Evolution/ Biological Diversity CHAPTERS
32-Introduction to Animal Diversity. Themes: 1,2,4,5,7 Skills: PP “Parade Through The
Kingdoms”, *Animal Diversity Lab I.
Describe the origins of animal diversity.
33-Invertebrates. Themes: 2,4,6 Skills: *Animal Diversity Lab II.
Compare and contrast organisms found in phylum Porifera to the
Eumetazoans. 34-Vertebrates. Themes: 2,4,5,7.
Describe the characteristics that classify organisms as chordates.
Assignments and assessments: Read all chapters, “Parade Through The Kingdom” study
guide, labs, Animal Lab Exam I and II.
UNITS: Organisms and Populations.
TOPIC: Animal Form and Function CHAPTERS
40-Animal Form and Function.Themes: 2,3,4,5,6,7.
Identify characteristics that define animal form and function.
41-Animal Nutrition: Themes. 3,5.
Compare and contrast different animal digestive systems.
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42-Circulation and Gas Exchange. Themes: 3,5,6,8 Skills: CB Physiology of the
Circulatory System, *EKG Labpro/sensor Lab, Q10 Goldfish Lab.
Describe gas exchange in different animals.
43-Immune System. Themes: 3,5,6,8.
Distinguish between the different components of the immune system.
44-Osmoregulation and Osmosis. Themes: 3,5,6,8.
Explain how water balance and waste disposal are accomplished in
different organisms.
Assignments and assessments: Read all chapters, labs, Systems Exam I and II.
MONTH: April and May.
UNIT: Organisms and Populations.
TOPIC: Animal Form and Function. CHAPTERS
45-Hormones and the Endocrine System. Themes: 3,5,6,8.
Distinguish between various chemical signals and their functions.
47-Animal Development. Themes: 3,5,6,8.
Describe the stages of embryonic development in animals.
48-Nervous Systems. Themes: 3,5,6,8 Skills: *Nervous Tissue and Vertebrate Eye Lab.
Describe the vertebrate nervous system.
49-Sensory and Motor Mechanisms. Themes: 3,5,6,8 Skills: *Fetal Pig Dissection Lab.
Compare and contrast sensory and motor mechanisms for different
animals. Assignments and assessments: Read all chapters, labs, Systems Exam III and IV.
SEMESTER II FETAL PIG LAB PRACTICAL
REVIEW!
Themes for individual topics/chapters may vary from those identified. CB=College
Board Lab, * Additional Lab, PP=”Paper and Pencil” Lab activity. This year-long plan
can be adjusted at any time and is a work in progress.
LABORATORY EXERCISES AND OBJECTIVES
1) Diffusion and Osmosis (College Board)
-Investigate the processes of diffusion and osmosis in a model membrane system.
-Investigate the effect of solute concentration on water potential as it relates to living
plant tissue.
*2-70 minute periods. Student conducted, hands-on wet lab.
2) Enzyme Catalysis (College Board)
-Observe the conversion of hydrogen peroxide to water and oxygen gas by the enzyme
catalase.
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-Measure the amount of oxygen generated and calculate the rate of enzyme-catalyzed
reaction.
* 2-70 minute periods. Student conducted, hands-on wet lab.
3) Mitosis and Meiosis (College Board)
-Investigate plant mitosis and calculate the relative duration of the phases of mitosis in
the meristem of root tissue.
-Compare animal and plant mitosis.
-Simulate the phases of meiosis using chromosome models.
-Observe the arrangement of ascospores in the asci from a cross between wild and mutant
type Sordaria cultures.
-Estimate the percentage of crossing over that occurs between the centromere and the
gene that controls the tan spore color.
*Mitosis lab, 1-50 minute period. Student conducted, hands-on wet lab.
*Meiosis lab models, 1-50 minute period. Student conducted, hands-on wet lab.
*Sordaria lab, 1-50 minute period, 1-70 minute period. Student conducted, hands-on wet
lab.
4) Plant Pigments and Photosynthesis (College Board)
-Separate plant pigments using chromatography.
-Measure the rate of photosynthesis in isolated chloroplasts.
*2-70 minute periods. Student conducted, hands-on wet lab.
5) Cell Respiration (College Board)
-Measure oxygen consumption during respiration.
-Measure the change in gas volume in respirometers containing either germinating or
nongerminating pea seeds.
-Measure the rate of respiration of these peas at two different temperatures. *2-70 minute periods. Student conducted, hands-on wet lab.
6) Molecular Biology (College Board)
-Transform E.Coli cells using plasmids containing fragments of foreign DNA, conferring
antibiotic resistance.
-Demonstrate techniques for separating and identifying DNA fragments from restriction
enzyme digests of lambda DNA using gel electrophoresis.
*Transformation lab, 2-70 minute periods. Student conducted, hands-on wet lab.
*Gel Electrophoresis lab, 2-70 minute periods. Student conducted, hands-on wet lab.
7) Genetics of Organisms (College Board)
-Observe a genetic cross using Drosophila.
-Analyze the results of a sex-linked cross using chi-square.
*2-70 minute periods, 2-50 minute periods. Student conducted, hands-on wet lab.
8) Population Genetics and Evolution (College Board)
-Learn about the Hardy-Weinberg law of genetic equilibrium and study the relationship
between evolition and changes in allele frequency in a class sample.
*1-70 minute period. Student conducted, hands-on dry lab.
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9) Transpiration (College Board)
-Apply prior knowledge of water potential from diffusion and osmosis to the movement
of water within the plant.
-Measure transpiration under different lab conditions.
-Study the organization of the plant stem and leaf as it relates to transpiration.
*1-70 minute period. Student conducted, hands-on wet lab.
10) Physiology of the Circulatory System (College Board)
-Measure blood pressure.
-Measure pulse rate and blood pressure, analyzing and relating it to an index of relative
fitness.
*1-70 minute period. Student conducted, hands-on dry lab.
11) Animal Behavior (College Board)
-Observe pillbugs and design an experiment to investigate their responses to
environmental variables.
*2-70 minute periods. Student generated and conducted, hands-on wet lab.
12) Dissolved Oxygen and Aquatic Primary Productivity (College Board)
-Measure and analyze the dissolved oxygen concentration in water samples at varying
temperatures.
-Measure and analyze the primary productivity of natural waters using screens to
simulate the attenuation of light with increasing depth.
*2-70 minute periods. Student conducted., hands-on wet lab.
13) Enzyme (Labpro/ pressure sensor, (Carolina Biological Inquiry-Based Labs)
-Investigate how temperature affects the rate of an enzymatic reaction using catalase,
pressure sensors, Labpros, and graphing calculators.
*1-70 minute period. Student generated and conducted, hands-on wet lab.
14) Photosynthesis- Labpro (PH probe) Algae or Lemna minor/rate of photosynthesis.
(Carolina Biological Inquiry-Based Labs)
-Investigate the rate of photosynthesis using ph probe Labpro®, graphing calculator, and
different light filters (red, blue, green cellophane).
*2-70 minute periods. Student generated and conducted, hands-on wet lab.
15) Transformation/ Glow-in-the-dark, E. coli. (Ward’s Natural Science)
-Transform E.Coli cells using plasmids containing fragments of foreign DNA, for
luminescence.
1-70 , 1-30 minute periods. Student conducted, hands-on wet lab.
17) Plant Hormones Fast plants. (Wisconsin Fast Plants)
-Investigate the affects of gibberellic acid on wild type and mutant type Brassica plants.
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*2-50, 3-10 minute periods. Student conducted, hands-on wet lab.
18) Fertilizer Runoff and Primary Productivity. (Carolina Biological Inquiry-Based
Labs)
-Investigate how fertilizer runoff affects the primary productivity of a body of water by
sampling dissolved oxygen.
*2-70 minute periods. Student generated and conducted, hands-on wet lab.
19) Carbohydrate, unknowns. (Source unknown)
-Identify unknown samples of monosaccharides, disaccharides, and polysaccharides
using indicators.
*1-70 minute period. Student conducted, hands-on wet lab.
20) Root/stem/leaf-monoct vs dicot. (Logsdon)
-Compare and contrast prepared microscope slides of monocot and dicot samples.
*2-50 minute periods. Student conducted, hands-on wet lab.
21) Protists prepared. (Logsdon)
-Compare and contrast prepared slides of different examples of protozoans.
*2-50 minute periods. Student conducted, hands-on dry lab
22) Human Populations. (Source unknown)
-Sample newspaper obituaries and create age-based survivorship curves for males and
females.
*1-50 minute period. Student conducted, hands-on dry lab.
23) Paper and pencil Paper Plasmids. (National Association of Biology Teachers)
-Simulate the transformation of genes using paper and pencil.
*1-50 minute period. Student conducted, hands-on dry lab.
24) Paper and pencil Toothpickase. (Dwane Grace)
-Simulate the action of enzymes on a substrate using toothpicks, and calculating the rate
of reaction.
1-20 minute period. Student conducted, hands-on dry lab.
25) Bacteria/Cyanobacteria (Live) Microscope Lab Prepared Slides. (Logsdon)
-Compare and contrast the various shapes of bacteria and types of cyanobacteria.
*1-50 minute period. Student conducted, hands-on wet/dry lab.
26) Fetal Pig dissection. (Logsdon)
-Identify the muscles, bones, and organs, of a fetal pig using a dissected pig.
-Compare and contrast pig body systems to human body systems.
*6-50 minute periods. Student conducted, hands-on wet lab.
27) Nervous Tissue, the reflex arc and vertebrate eye. (Morgan and Carter)
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-Describe the pathway of a simple reflex, relating this to the structure of the spinal cord.
-Describe the structures of a sheep eye through dissection.
*1-70 minute period. Student conducted, hands-on wet lab.
28) Animal Diversity Lab I and II (Morgan and Carter)
-Compare the anatomy of representative animals, describing similarities and differences
in organs and body forms.
-Discuss the relationship of form and function and how this relates to the organisms
interactions with the environment.
*3-70 minute periods, 2-50 minute periods. Student conducted, wet/dry lab.
29) EKG Lab. EKG sensor and computer lab. (Biology with Computers)
-Record and analyze student EKG’s using Vernier probes and Loggerpro software.
*1-50 minute period. Student conducted, hands-on wet lab.
30) Restriction Analysis Lab, paper and pencil. (Carolina Biological Inquiry-Based
Labs)
-Simulate the use of restriction enzymes in cutting DNA.
*1-50 minute period. Student conducted, hands-on dry lab.
31) Grocery Store Botany. (Morgan and Carter)
-Apply your knowledge of plants and plant morphology to the kinds of fruits and
vegetables that are found in the grocery store.
*1-50 minute period. Student conducted, hands-on wet lab.
32) Q10 Goldfish Lab. (Mike Nolan, www.bioimmunity).
-Calculate the Q10 for goldfish under different temperatures and compare.
Citations:
Carolina Biological Supply Company, Andrews Sara, 2000. “Advanced Placement
Biology Inquiry-Based Lab Manual”.
Carolina Biological Supply Company, Wisconsin Fast Plants, 1989. “ The Effectsof
Gibberellic Acid on Wild-type and Rossette Plants”. Carter Brown, M. , Morgan, J. “Investigating Biology Lab Manual, 5th ed. 2005.
College Board, 2006-2007. “Biology Course Description”.
Vernier, Masterman and Redding, 2006. “Biology with Computers”, 3rd ed. 2006.
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Ward’s Natural Science, 2002. “ Glowing Bacteria: Transformation with a Firefly Gene
Lab Activity”.
CB/ Diffusion and Osmosis
CB/ Enzyme Catalysis
LABORATORIES
*Enzymes- Labpro (pressure probes)/Temperature
CB Meiosis/ *Sordaria meiosis
CB Plant Pigments and Photosynthesis
*Photosynthesis- Labpro (PH probe) Algae/rate of photo
CB Cellular Respiration CB Molecular Biology
a. Transformation PBLU
b. Forensics lab/Restriction enzymes
*Transformation/ Glow-in-the Dark E.coli
*Bacterial Congugation- at Ohio State University Field Day
CB Genetics of Organisms/ Drosophilia sex-linked cross
*Plant Hormones Fast plants/ soybean
CB Mitosis
CB Physiology of the Circulatory System/ Goldfish Q10
CB Transpiration
CB Dissolved Oxygen and Aquatic Primary Productivity
*Fertilizer Runoff and Primary Productivity
*Carbohydrates, unknowns
CB Population Genetics and Evolution
CB Animal Behavior
*Root/Stem/Leaf monocot/dicot
*Protists, prepared
PP Fish population
PP Human populations
PP Paper plasmids.
PP Toothpickase
*Bacteria/Cyanobacteria (Live) Microscope Lab Prepared Slides
*Fetal Pig dissection
PP Restriction Analysis
*Properties of water demonstration.
Extra labs time permitting:
*PCR (forensics)
*Signal Transduction, soybean pathogenic response
*Aggressive behavior in crickets