Post on 11-Apr-2017
Uptakes of SPIO by Murine Macrophages and Monocytes
Daniel ChanUniversity of Colorado Health Sciences Center
12-19-2001
Synthesis of SPIO
• 1. SPIO coated with chondroitin sulfate• 2 SPIO coated with standard dextran
(6K)• 3 SPIO coated with Amino-silane• 4 SPIO coated with lipids• 5 Lipid encapsulated SPIO coated with
amino-silane
Uptakes of SPIOs by murine peritoneal macrophages and monocytes
• Normal mice were treated with mineral oil by ip injection.
• 24 hours later, equal amount of various SPIOs were injected intraperitoneally.
• 24 hours later, peritoneal macrophages and monocytes were isolated.
• Uptakes of various SPIOs were examined by light microscopy.
Uptake by Mouse Macrophages
SPIO coated with chondroitin sulfate
Uptake by Mouse Macrophages
SPIO coated with standard Dextran (6K)
Uptake by Mouse Macrophages
SPIO coated with amino-silane
Uptake by Mouse Macrophages
SPIO coated with lipids
Uptake by Mouse Macrophages
Lipid encapsulated SPIO coated with amino-silane
Uptake by Mouse macrophages
SPIO coated with CS
Uptake by Mouse Macrophages
SPIO coated with Dextran (6k)
Uptake by Mouse Macrophages
SPIO coated with Amino-silane
Uptake by Mouse Macrophages
SPIO coated with lipids
Uptake by Mouse Macrophages
Lipid encapsulated SPIO coated with amino-silane